Summary of Study ST000510

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000382. The data can be accessed directly via it's Project DOI: 10.21228/M8P597 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000510
Study TitleAssessing metabolic changes to maternal rat liver tissue during and post-pregnancy (part II)
Study SummaryAssessing metabolic changes to maternal rat liver tissue during and post-pregnancy. Liver tissue was harvested post-mortem from rats or mice without pregnancy (Nulliparous - NP), time course during pregnancy in days (P2-4, P11-13m P18-20), lactation day 10 (LD10), in during involution of the liver (I2, I4, I6, I8, I10) and 4-weeks regression after preganancy (R4).
Institute
University of Colorado Denver
Last NameNemkov
First NameTravis
Address12801 E 17th Avenue Mail Stop 8101
Emailtravis.nemkov@ucdenver.edu
Phone3037245798
Submit Date2016-12-05
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2017-01-30
Release Version1
Travis Nemkov Travis Nemkov
https://dx.doi.org/10.21228/M8P597
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000382
Project DOI:doi: 10.21228/M8P597
Project Title:Metabolic changes to maternal rat liver tissue during and post-pregnancy.
Project Type:global MS analysis
Project Summary:Assessing metabolic changes to maternal rat liver tissue during and post-pregnancy. Liver tissue was harvested post-mortem from rats or mice without pregnancy (Nulliparous - NP), time course during pregnancy in days (P2-4, P11-13m P18-20), lactation day 10 (LD10), in during involution of the liver (I2, I4, I6, I8, I10) and 4-weeks regression after preganancy (R4).
Institute:University of Colorado Denver
Last Name:Nemkov
First Name:Travis
Address:12801 E 17th Avenue, RC-1 South, Rm 9403G, Aurora, CO, 80045, USA
Email:travis.nemkov@ucdenver.edu
Phone:303-724-5798

Subject:

Subject ID:SU000532
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id time_point
SA026556I2_17I2
SA026557I2_224I2
SA026558I2_39I2
SA026559I2_34I2
SA026560I2_31I2
SA026561I2_229I2
SA026562I4_18I4
SA026563I4_215I4
SA026564I4_211I4
SA026565I4_202I4
SA026566I4_13I4
SA026567I4_15I4
SA026568I6_209I6
SA026569I6_218I6
SA026570I6_230I6
SA026571I6_232I6
SA026572I6_201I6
SA026573I6_216I6
SA026574I8_14I8
SA026575I8_99bI8
SA026576I8_98bI8
SA026577I8_101bI8
SA026578LD10_213LD10
SA026579LD10_236LD10
SA026580LD10_240LD10
SA026581LD10_237LD10
SA026582LD10_204LD10
SA026583LD10_200LD10
SA026584NP_226NP
SA026585NP_221NP
SA026586NP_227NP
SA026587NP_225NP
SA026588NP_220NP
SA026589NP_222NP
SA026590R4_243R4
SA026591R4_239R4
SA026592R4_214R4
SA026593R4_203R4
SA026594R4_232R4
Showing results 1 to 39 of 39

Collection:

Collection ID:CO000526
Collection Summary:tissue was harvested, flash frozen in LiN2, milled with LiN2, and weighed on dry ice for metabolomics analysis
Sample Type:Liver

Treatment:

Treatment ID:TR000546
Treatment Summary:Tissues were harvested at the appropriate time point during or post-pregnancy

Sample Preparation:

Sampleprep ID:SP000539
Sampleprep Summary:Frozen and milled liver tissue was suspended to 10 mg/ml in an extraction solution containing methanol/acetonitrile/water at a ratio of 5:3:2 (v/v/v). Suspensions were vortexed at 4C for 30 minutes and centrifuged at 10,000 g for 10 minutes, 4C. Supernatants were isolated and analyzed by LC-MS.

Combined analysis:

Analysis ID AN000782 AN000783
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Kinetex C18-XB Kinetex C18-XB
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units arbitrary units arbitrary units

Chromatography:

Chromatography ID:CH000560
Instrument Name:Thermo Vanquish
Column Name:Kinetex C18-XB
Chromatography Type:Reversed phase

MS:

MS ID:MS000689
Analysis ID:AN000782
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000690
Analysis ID:AN000783
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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