Summary of Study ST001214

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000811. The data can be accessed directly via it's Project DOI: 10.21228/M8VD62 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001214
Study TitleLipidommics in the serum of human subjects
Study SummaryWe aimed to determine the levels of the cold-induced 12-LOX products in patients with different degrees of body mass index (BMI). This analysis allowed us to infer about the role of these oxylipins in the pathophysiology of obesity.
Institute
Joslin Diabetes Center
Last NameLeiria
First NameLuiz
AddressOne Joslin Place, 02215, Boston-MA - USA
Emailluiz.leiria@joslin.harvard.edu
Phone617-309-1967
Submit Date2019-07-10
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2019-09-23
Release Version1
Luiz Leiria Luiz Leiria
https://dx.doi.org/10.21228/M8VD62
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000811
Project DOI:doi: 10.21228/M8VD62
Project Title:12-LOX metabolites in cold adaptation
Project Summary:The goal of this project is to understand the role of the enzyme 12-lipoxygenase in the adaptive thermogenesis. We found this enzyme is activated by cold stimulation, then producing lipid metabolites in adipose tissue and releasing them into the circulation to regulate fuel utilisation and thermogenic pathways required for the cold adaptation.
Institute:Joslin Diabetes Center
Department:Integrative Physiology and Metabolism
Laboratory:Yu-Hua Tseng lab
Last Name:Leiria
First Name:Luiz
Address:One Joslin Place, Boston, MA-USA, 02215
Email:luiz.leiri@joslin.harvard.edu
Phone:1 6173091967

Subject:

Subject ID:SU001281
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id BMI group
SA08583114-0lean
SA08583213-0lean
SA08583316-0lean
SA08583420-0lean
SA0858351-0lean
SA08583612-0lean
SA08583718-0lean
SA08583817-0lean
SA0858394-0lean
SA08584011-0lean
SA0858413-0lean
SA0858426-0lean
SA0858435-0lean
SA0858447-0lean
SA0858458-0lean
SA0858462310obese
SA0858472444obese
SA0858482655obese
SA0858492340obese
SA0858502678obese
SA0858512276obese
SA0858522548obese
SA0858532634obese
SA0858542299obese
SA0858553692obese
SA0858562346obese
SA0858572385obese
SA0858582227obese
SA0858592054obese
SA0858602667obese
SA0858612720obese
SA0858622727obese
SA0858632225obese
SA0858642465obese
SA0858652184obese
SA0858662724obese
SA0858672505obese
SA0858683694obese
SA0858692739obese
SA0858702438obese
SA0858712614obese
SA0858722744obese
SA0858732734obese
SA0858742404obese
SA0858752595obese
SA0858762524obese
SA0858772446overweight
SA0858781570overweight
SA0858791021overweight
SA0858801053overweight
SA0858811016overweight
SA0858821082overweight
SA0858831308overweight
SA0858841508overweight
SA0858852701overweight
SA0858861363overweight
SA0858872371overweight
SA0858881630overweight
SA0858892341overweight
SA0858901651overweight
Showing results 1 to 60 of 60

Collection:

Collection ID:CO001275
Collection Summary:Blood was collected from the subjects and serum obtained by fractionation. Collection of human biomaterial, serum analyses and phenotyping were approved by the ethics committee of the University of Leipzig (approval numbers: 159-12-21052012 and 017-12-23012012), and all individuals gave written informed consent before taking part in the study.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR001296
Treatment Summary:A cohort of 55 individuals were selected from the Leipzig biobank (42 women and 13 men) to represent a wide range of BMI (17.5–75.4 kg/m2), categories of lean (BMI < 25 kg/m2; n = 15; 4 male (M)/11 female (F)), overweight (BMI 25.1–29.9 kg/m2; n = 13; 4 M/9 F) or obese (BMI > 30 kg/m2; n = 27; 5 M/22 F) and glucose-metabolism parameters (fasting plasma glucose 3.9–13.4 mmol/liter; fasting plasma insulin 3.8–451 pmol/liter, HOMA-IR: 0.1–25). In the subgroup of lean, all individuals were normal glucose tolerant (NGT), whereas in the overweight subgroup, 10 individuals with NGT and 3 with type 2 diabetes (T2D), and in the obese group, 20 NGT individuals and 7 individuals with T2D were included.

Sample Preparation:

Sampleprep ID:SP001289
Sampleprep Summary:Aliquots of 100 µL serum were taken, depending on the experiment. A mixture of deuterium-labeled internal standards was added to each aliquot, followed by 3x volume of sample of cold methanol (MeOH). Samples were vortexed for 5 minutes and stored at 31 −20 °C overnight. Cold samples were centrifuged at 14,000g for 10 minutes, and the supernatant was then transferred to a new tube and 3 mL of acidified H 2 O (pH 3.5) was added to each sample prior to C18 SPE. The methyl formate fractions were collected, dried under nitrogen, and reconstituted in 50 µL MeOH:H 2 O (1:1, by vol). Samples were transferred to 0.5 mL tubes and centrifuged at 20,000g at 4 °C for 10 minutes. (35ul) of supernatant was transferred to LC–MS/MS vials for analysis using the BERG LCMS/MS mediator lipidomics platform.

Combined analysis:

Analysis ID AN002025
Analysis type MS
Chromatography type Reversed phase
Chromatography system Ekspert MicroLC 200 system
Column Phenomenex Synergi Fusion-RP capillary C18 (150 × 0.5 mm,4um)
MS Type ESI
MS instrument type QTOF
MS instrument name ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE
Units Peak Area

Chromatography:

Chromatography ID:CH001466
Instrument Name:Ekspert MicroLC 200 system
Column Name:Phenomenex Synergi Fusion-RP capillary C18 (150 × 0.5 mm,4um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001878
Analysis ID:AN002025
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:MS analysis was performed on a SCIEX TripleTOF® 5600+ system using the HR-MRM strategy consisting of a TOF MS experiment looped with multiple MS/MS experiments. MS spectra were acquired in high-resolution mode (>30,000) using a 100-ms accumulation time per spectrum. Fullscan MS/MS was acquired in high sensitivity mode, with an accumulation time optimized per cycle. Collision energy was set using rolling collision energy with a spread of 15V. The identity of a component was confirmed using PeakView® software (SCIEX), and quantification was performed using MultiQuant™ software (SCIEX).
Ion Mode:NEGATIVE
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