{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000706","ANALYSIS_ID":"AN001097","VERSION":"1","CREATED_ON":"July 2, 2017, 11:07 am"}, "PROJECT":{"PROJECT_TITLE":"Effect of DASH Diet on Gut Microbiome","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"Effect of DASH Diet on Gut Microbiome","INSTITUTE":"Morehouse School of Medicine","DEPARTMENT":"Clinical Research Center","LABORATORY":"Umeakunne Lab","LAST_NAME":"Umeakunne","FIRST_NAME":"Kayellen","ADDRESS":"Atlanta, GA","EMAIL":"kumeakunne@msm.edu","PHONE":"404-752-1010"}, "STUDY":{"STUDY_TITLE":"Effect of DASH Diet on Gut Microbiome (SCFA from stool)","STUDY_TYPE":"MS analysis","STUDY_SUMMARY":"This research will examine the effect of the currently recommended DASH diet versus a Vegetarian DASH diet on the gut microbiome and risk for cardiovascular disease in pre-hypertensive obese African American women. We will define the gut microbiota profile, short chain fatty acid production, breath hydrogen/methane response, plasma lipopolysaccharide production and other biomarkers of inflammation in response to diet type in obese African American pre-hypertensive females at baseline and following placement on the traditional DASH plan or DASH Vegetarian diet. We will also define these parameters in African American women adhering to a Vegetarian or Vegan Diet. Our goal is to determine how the gut microbiome modulates host physiology and immune function in response to diet type. By evaluating the effect of a recommended DASH dietary pattern versus a Vegetarian DASH plan on the gut microbiome and its fermentation products, we aim to identify novel information about how these diet types strategically reduce cardiovascular disease risk through gut:microbiota:host interaction.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"5","TOTAL_SUBJECTS":"13","STUDY_COMMENTS":"We will define the gut microbiota profile, short chain fatty acid production, breath hydrogen/methane response, plasma lipopolysaccharide production and other biomarkers of inflammation in response to diet type in obese African American pre-hypertensive females at baseline and following placement on the traditional DASH plan or DASH Vegetarian diet. We will also define these parameters in African American women adhering to a Vegetarian or Vegan Diet. Our goal is to determine how the gut microbiome modulates host physiology and immune function in response to diet type. By evaluating the effect of a recommended DASH dietary pattern versus a Vegetarian DASH plan on the gut microbiome and its fermentation products, we aim to identify novel information about how these diet types strategically reduce cardiovascular disease risk through gut:microbiota:host interaction."}, "SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"SU0013361", "Sample ID":"S00019911", "Factors":{"GROUP":"pre-hypertensive","Timepoint":"Baseline"} }, { "Subject ID":"SU0013361", "Sample ID":"S00019912", "Factors":{"GROUP":"pre-hypertensive","Timepoint":"Final"} }, { "Subject ID":"SU0013362", "Sample ID":"S00019913", "Factors":{"GROUP":"pre-hypertensive","Timepoint":"Baseline"} }, { "Subject ID":"SU0013362", "Sample ID":"S00019914", "Factors":{"GROUP":"pre-hypertensive","Timepoint":"Final"} }, { "Subject ID":"SU0013363", "Sample ID":"S00019915", "Factors":{"GROUP":"vegetarian","Timepoint":"NA"} }, { "Subject ID":"SU0013364", "Sample ID":"S00019916", "Factors":{"GROUP":"vegetarian","Timepoint":"NA"} }, { "Subject ID":"SU0013365", "Sample ID":"S00019917", "Factors":{"GROUP":"vegetarian","Timepoint":"NA"} }, { "Subject ID":"SU0013366", "Sample ID":"S00019918", "Factors":{"GROUP":"vegetarian","Timepoint":"NA"} }, { "Subject ID":"SU0013367", "Sample ID":"S00019919", "Factors":{"GROUP":"vegetarian","Timepoint":"NA"} }, { "Subject ID":"SU0013368", "Sample ID":"S00019920", "Factors":{"GROUP":"vegetarian","Timepoint":"NA"} }, { "Subject ID":"SU0013369", "Sample ID":"S00019921", "Factors":{"GROUP":"vegetarian","Timepoint":"NA"} }, { "Subject ID":"SU0013370", "Sample ID":"S00019922", "Factors":{"GROUP":"vegetarian","Timepoint":"NA"} }, { "Subject ID":"SU0013371", "Sample ID":"S00019923", "Factors":{"GROUP":"vegetarian","Timepoint":"NA"} }, { "Subject ID":"SU0013372", "Sample ID":"S00019924", "Factors":{"GROUP":"vegetarian","Timepoint":"NA"} }, { "Subject ID":"SU9999996", "Sample ID":"S00020331", "Factors":{"GROUP":"-","Timepoint":"-"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"-"}, "TREATMENT":{"TREATMENT_SUMMARY":"-"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"A007 - Short Chain Fatty Acids.pdf"}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890N","COLUMN_NAME":"Phenomenex Zebron ZB-WAXplus (30m x 0.25mm, 0.25um)"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"A007 - Short Chain Fatty Acids.pdf"}, "MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 5975","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE"}, "MS_METABOLITE_DATA":{ "Units":"nmol/mg", "Data":[{"Metabolite":"PROPIONIC ACID","S00019911":"8.852236798","S00019912":"11.27271996","S00019913":"17.03812961","S00019914":"14.78126926","S00019915":"16.36067918","S00019916":"13.96667136","S00019917":"16.44197152","S00019918":"8.884039523","S00019919":"9.481304533","S00019920":"17.32633263","S00019921":"28.31857223","S00019922":"14.32819129","S00019923":"13.30631982","S00019924":"19.99695861"},{"Metabolite":"ISOVALERIC ACID","S00019911":"3.457113334","S00019912":"3.625089679","S00019913":"2.291167277","S00019914":"2.779638021","S00019915":"2.181763109","S00019916":"3.057268574","S00019917":"3.120600567","S00019918":"2.41064069","S00019919":"6.203278222","S00019920":"2.733310428","S00019921":"3.791872433","S00019922":"2.8387834","S00019923":"2.158547839","S00019924":"3.654262637"},{"Metabolite":"ACETIC ACID","S00019911":"18.56150626","S00019912":"26.61320737","S00019913":"42.68108337","S00019914":"39.37739033","S00019915":"35.95992971","S00019916":"50.69160271","S00019917":"65.33690694","S00019918":"64.04033935","S00019919":"51.95017774","S00019920":"53.32053031","S00019921":"59.27061884","S00019922":"39.8394841","S00019923":"70.73166133","S00019924":"56.88307069"},{"Metabolite":"BUTYRIC ACID","S00019911":"6.915580233","S00019912":"10.13901386","S00019913":"15.81784213","S00019914":"10.65616711","S00019915":"10.08362003","S00019916":"15.02419468","S00019917":"10.23049748","S00019918":"11.54838117","S00019919":"11.33329436","S00019920":"15.52744651","S00019921":"15.1886497","S00019922":"10.21575242","S00019923":"13.58832323","S00019924":"11.69908892"},{"Metabolite":"VALERIC ACID","S00019911":"3.577568723","S00019912":"3.88116141","S00019913":"2.201813285","S00019914":"2.783312711","S00019915":"2.817962031","S00019916":"3.882171896","S00019917":"3.706071313","S00019918":"2.95050583","S00019919":"6.367080467","S00019920":"3.280202867","S00019921":"4.69186696","S00019922":"3.418173322","S00019923":"2.516136008","S00019924":"4.785035801"},{"Metabolite":"HEPTANOIC ACID","S00019911":".036158188","S00019912":".029603602","S00019913":".025142999","S00019914":".031193913","S00019915":".020295859","S00019916":".11874462","S00019917":".032062447","S00019918":".023965516","S00019919":".030655009","S00019920":".024707107","S00019921":".032089551","S00019922":".081992259","S00019923":".024006212","S00019924":".02338342"},{"Metabolite":"HEXANOIC ACID","S00019911":".66161492","S00019912":".542445035","S00019913":".449052572","S00019914":".589460473","S00019915":".39309608","S00019916":"1.838362861","S00019917":".639891077","S00019918":".45582264","S00019919":".559459378","S00019920":".476105594","S00019921":".6042068","S00019922":"1.193949944","S00019923":".460094135","S00019924":".450508403"}], "Metabolites":[{"Metabolite":"PROPIONIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"1032","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ISOVALERIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"10430","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ACETIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"176","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"BUTYRIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"264","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"VALERIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"7991","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"HEPTANOIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"8094","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"HEXANOIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"8892","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""}] } }