{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000950","ANALYSIS_ID":"AN001559","VERSION":"1","CREATED_ON":"April 18, 2018, 3:26 pm"}, "PROJECT":{"PROJECT_TITLE":"NK versus CD8 T naïve and CD8 T memory","PROJECT_SUMMARY":"Exp to look at lactate/ alanine excreted in supernatant, lactate, citrulline and other TCA intermediates inside cells","INSTITUTE":"University of Kentucky","DEPARTMENT":"Markey Cancer Center","LAST_NAME":"Lutz","FIRST_NAME":"Charlie","ADDRESS":"Dept Of Pathology, UKMC","EMAIL":"ctlutz2@uky.edu","PHONE":"000-000-0000"}, "STUDY":{"STUDY_TITLE":"Human NK vs T cell metabolism using 13C-Glucose tracer with/out galactose (part II)","STUDY_TYPE":"isotope tracer","STUDY_SUMMARY":"NK versus CD8 T naïve and CD8 T memory SIRM experiment using 13C-Glucose tracer with/out galactose","INSTITUTE":"University of Kentucky","DEPARTMENT":"Markey Cancer Center","LAST_NAME":"Presnell","FIRST_NAME":"Steve","ADDRESS":"Dept Of Pathology, UKMC","EMAIL":"srpres2@uky.edu","PHONE":"000-000-0000","STUDY_COMMENTS":"For additional comments about the study, like listing a publication."}, "SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"-", "Sample ID":"20150602_NK_plate1-cells", "Factors":{"cell_type":"NK"}, "Additional sample data":{"protocol.id":"normal_12CGlc","lineage_1":"Donor_SRP","protocol.id":"normal","species":"Homo sapiens","species_type":"Animal","taxonomy_id":"9606","lineage_2":"Donor_SRP_blood_20150602","protocol.id":"blood_collection","lineage_3":"Donor_SRP_blood_20150602_NK","cell_count":"1800000","cell_type":"NK","protocol.id":"cell_extraction","lineage_4":"Donor_SRP_blood_20150602_NK_plate1","cell_type":"NK","plate_media_tare":"-1","plate_media_tare%units":"g","protocol.id":"normal_12CGlc","replicate":"1","replicate%type":"biological","lineage_5":"Donor_SRP_blood_20150602_NK_plate1-cells","cell_type":"NK","protocol.id":"cell_quench_collection","lineage_6":"Donor_SRP_blood_20150602_NK_plate1-cells-polar_GCMS_A","protocol.id":"polar_extraction","replicate":"1","replicate%type":"analytical","type":"polar","weight":"-1","weight%units":"g"} }, { "Subject ID":"-", "Sample ID":"20150602_NK_plate2-cells", "Factors":{"cell_type":"NK"}, "Additional sample data":{"protocol.id":"galactose_12CGlc","lineage_1":"Donor_SRP","protocol.id":"normal","species":"Homo sapiens","species_type":"Animal","taxonomy_id":"9606","lineage_2":"Donor_SRP_blood_20150602","protocol.id":"blood_collection","lineage_3":"Donor_SRP_blood_20150602_NK","cell_count":"1800000","cell_type":"NK","protocol.id":"cell_extraction","lineage_4":"Donor_SRP_blood_20150602_NK_plate2","cell_type":"NK","plate_media_tare":"-1","plate_media_tare%units":"g","protocol.id":"galactose_12CGlc","replicate":"1","replicate%type":"biological","lineage_5":"Donor_SRP_blood_20150602_NK_plate2-cells","cell_type":"NK","protocol.id":"cell_quench_collection","lineage_6":"Donor_SRP_blood_20150602_NK_plate2-cells-polar_GCMS_A","protocol.id":"polar_extraction","replicate":"1","replicate%type":"analytical","type":"polar","weight":"-1","weight%units":"g"} }, { "Subject ID":"-", "Sample ID":"20150602_NK_plate3-cells", "Factors":{"cell_type":"NK"}, "Additional sample data":{"protocol.id":"normal_13CGlc","lineage_1":"Donor_SRP","protocol.id":"normal","species":"Homo sapiens","species_type":"Animal","taxonomy_id":"9606","lineage_2":"Donor_SRP_blood_20150602","protocol.id":"blood_collection","lineage_3":"Donor_SRP_blood_20150602_NK","cell_count":"1800000","cell_type":"NK","protocol.id":"cell_extraction","lineage_4":"Donor_SRP_blood_20150602_NK_plate3","cell_type":"NK","plate_media_tare":"-1","plate_media_tare%units":"g","protocol.id":"normal_13CGlc","replicate":"1","replicate%type":"biological","lineage_5":"Donor_SRP_blood_20150602_NK_plate3-cells","cell_type":"NK","protocol.id":"cell_quench_collection","lineage_6":"Donor_SRP_blood_20150602_NK_plate3-cells-polar_GCMS_A","protocol.id":"polar_extraction","replicate":"1","replicate%type":"analytical","type":"polar","weight":"-1","weight%units":"g"} }, { "Subject ID":"-", "Sample ID":"20150602_NK_plate4-cells", "Factors":{"cell_type":"NK"}, "Additional sample data":{"protocol.id":"galactose_13CGlc","lineage_1":"Donor_SRP","protocol.id":"normal","species":"Homo sapiens","species_type":"Animal","taxonomy_id":"9606","lineage_2":"Donor_SRP_blood_20150602","protocol.id":"blood_collection","lineage_3":"Donor_SRP_blood_20150602_NK","cell_count":"1800000","cell_type":"NK","protocol.id":"cell_extraction","lineage_4":"Donor_SRP_blood_20150602_NK_plate4","cell_type":"NK","plate_media_tare":"-1","plate_media_tare%units":"g","protocol.id":"galactose_13CGlc","replicate":"1","replicate%type":"biological","lineage_5":"Donor_SRP_blood_20150602_NK_plate4-cells","cell_type":"NK","protocol.id":"cell_quench_collection","lineage_6":"Donor_SRP_blood_20150602_NK_plate4-cells-polar_GCMS_A","protocol.id":"polar_extraction","replicate":"1","replicate%type":"analytical","type":"polar","weight":"-1","weight%units":"g"} }, { "Subject ID":"-", "Sample ID":"20150602_T_memory_plate10-cells", "Factors":{"cell_type":"CD8_T_memory"}, "Additional sample data":{"protocol.id":"galactose_12CGlc","lineage_1":"Donor_SRP","protocol.id":"normal","species":"Homo sapiens","species_type":"Animal","taxonomy_id":"9606","lineage_2":"Donor_SRP_blood_20150602","protocol.id":"blood_collection","lineage_3":"Donor_SRP_blood_20150602_T_memory","cell_count":"1570000","cell_type":"CD8_T_memory","protocol.id":"cell_extraction","lineage_4":"Donor_SRP_blood_20150602_T_memory_plate10","cell_type":"CD8_T_memory","plate_media_tare":"-1","plate_media_tare%units":"g","protocol.id":"galactose_12CGlc","replicate":"1","replicate%type":"biological","lineage_5":"Donor_SRP_blood_20150602_T_memory_plate10-cells","cell_type":"CD8_T_memory","protocol.id":"cell_quench_collection","lineage_6":"Donor_SRP_blood_20150602_T_memory_plate10-cells-polar_GCMS_A","protocol.id":"polar_extraction","replicate":"1","replicate%type":"analytical","type":"polar","weight":"-1","weight%units":"g"} }, { "Subject ID":"-", "Sample ID":"20150602_T_memory_plate11-cells", "Factors":{"cell_type":"CD8_T_memory"}, "Additional sample data":{"protocol.id":"normal_13CGlc","lineage_1":"Donor_SRP","protocol.id":"normal","species":"Homo sapiens","species_type":"Animal","taxonomy_id":"9606","lineage_2":"Donor_SRP_blood_20150602","protocol.id":"blood_collection","lineage_3":"Donor_SRP_blood_20150602_T_memory","cell_count":"1570000","cell_type":"CD8_T_memory","protocol.id":"cell_extraction","lineage_4":"Donor_SRP_blood_20150602_T_memory_plate11","cell_type":"CD8_T_memory","plate_media_tare":"-1","plate_media_tare%units":"g","protocol.id":"normal_13CGlc","replicate":"1","replicate%type":"biological","lineage_5":"Donor_SRP_blood_20150602_T_memory_plate11-cells","cell_type":"CD8_T_memory","protocol.id":"cell_quench_collection","lineage_6":"Donor_SRP_blood_20150602_T_memory_plate11-cells-polar_GCMS_A","protocol.id":"polar_extraction","replicate":"1","replicate%type":"analytical","type":"polar","weight":"-1","weight%units":"g"} }, { "Subject ID":"-", "Sample ID":"20150602_T_memory_plate12-cells", "Factors":{"cell_type":"CD8_T_memory"}, "Additional sample data":{"protocol.id":"galactose_13CGlc","lineage_1":"Donor_SRP","protocol.id":"normal","species":"Homo sapiens","species_type":"Animal","taxonomy_id":"9606","lineage_2":"Donor_SRP_blood_20150602","protocol.id":"blood_collection","lineage_3":"Donor_SRP_blood_20150602_T_memory","cell_count":"1570000","cell_type":"CD8_T_memory","protocol.id":"cell_extraction","lineage_4":"Donor_SRP_blood_20150602_T_memory_plate12","cell_type":"CD8_T_memory","plate_media_tare":"-1","plate_media_tare%units":"g","protocol.id":"galactose_13CGlc","replicate":"1","replicate%type":"biological","lineage_5":"Donor_SRP_blood_20150602_T_memory_plate12-cells","cell_type":"CD8_T_memory","protocol.id":"cell_quench_collection","lineage_6":"Donor_SRP_blood_20150602_T_memory_plate12-cells-polar_GCMS_A","protocol.id":"polar_extraction","replicate":"1","replicate%type":"analytical","type":"polar","weight":"-1","weight%units":"g"} }, { "Subject ID":"-", "Sample ID":"20150602_T_memory_plate9-cells", "Factors":{"cell_type":"CD8_T_memory"}, "Additional sample data":{"protocol.id":"normal_12CGlc","lineage_1":"Donor_SRP","protocol.id":"normal","species":"Homo sapiens","species_type":"Animal","taxonomy_id":"9606","lineage_2":"Donor_SRP_blood_20150602","protocol.id":"blood_collection","lineage_3":"Donor_SRP_blood_20150602_T_memory","cell_count":"1570000","cell_type":"CD8_T_memory","protocol.id":"cell_extraction","lineage_4":"Donor_SRP_blood_20150602_T_memory_plate9","cell_type":"CD8_T_memory","plate_media_tare":"-1","plate_media_tare%units":"g","protocol.id":"normal_12CGlc","replicate":"1","replicate%type":"biological","lineage_5":"Donor_SRP_blood_20150602_T_memory_plate9-cells","cell_type":"CD8_T_memory","protocol.id":"cell_quench_collection","lineage_6":"Donor_SRP_blood_20150602_T_memory_plate9-cells-polar_GCMS_A","protocol.id":"polar_extraction","replicate":"1","replicate%type":"analytical","type":"polar","weight":"-1","weight%units":"g"} }, { "Subject ID":"-", "Sample ID":"20150602_T_naive_plate5-cells", "Factors":{"cell_type":"CD8_T_naive"}, "Additional sample data":{"protocol.id":"normal_12CGlc","lineage_1":"Donor_SRP","protocol.id":"normal","species":"Homo sapiens","species_type":"Animal","taxonomy_id":"9606","lineage_2":"Donor_SRP_blood_20150602","protocol.id":"blood_collection","lineage_3":"Donor_SRP_blood_20150602_T_naive","cell_count":"2040000","cell_type":"CD8_T_naive","protocol.id":"cell_extraction","lineage_4":"Donor_SRP_blood_20150602_T_naive_plate5","cell_type":"CD8_T_naive","plate_media_tare":"-1","plate_media_tare%units":"g","protocol.id":"normal_12CGlc","replicate":"1","replicate%type":"biological","lineage_5":"Donor_SRP_blood_20150602_T_naive_plate5-cells","cell_type":"CD8_T_naive","protocol.id":"cell_quench_collection","lineage_6":"Donor_SRP_blood_20150602_T_naive_plate5-cells-polar_GCMS_A","protocol.id":"polar_extraction","replicate":"1","replicate%type":"analytical","type":"polar","weight":"-1","weight%units":"g"} }, { "Subject ID":"-", "Sample ID":"20150602_T_naive_plate6-cells", "Factors":{"cell_type":"CD8_T_naive"}, "Additional sample data":{"protocol.id":"galactose_12CGlc","lineage_1":"Donor_SRP","protocol.id":"normal","species":"Homo sapiens","species_type":"Animal","taxonomy_id":"9606","lineage_2":"Donor_SRP_blood_20150602","protocol.id":"blood_collection","lineage_3":"Donor_SRP_blood_20150602_T_naive","cell_count":"2040000","cell_type":"CD8_T_naive","protocol.id":"cell_extraction","lineage_4":"Donor_SRP_blood_20150602_T_naive_plate6","cell_type":"CD8_T_naive","plate_media_tare":"-1","plate_media_tare%units":"g","protocol.id":"galactose_12CGlc","replicate":"1","replicate%type":"biological","lineage_5":"Donor_SRP_blood_20150602_T_naive_plate6-cells","cell_type":"CD8_T_naive","protocol.id":"cell_quench_collection","lineage_6":"Donor_SRP_blood_20150602_T_naive_plate6-cells-polar_GCMS_A","protocol.id":"polar_extraction","replicate":"1","replicate%type":"analytical","type":"polar","weight":"-1","weight%units":"g"} }, { "Subject ID":"-", "Sample ID":"20150602_T_naive_plate7-cells", "Factors":{"cell_type":"CD8_T_naive"}, "Additional sample data":{"protocol.id":"normal_13CGlc","lineage_1":"Donor_SRP","protocol.id":"normal","species":"Homo sapiens","species_type":"Animal","taxonomy_id":"9606","lineage_2":"Donor_SRP_blood_20150602","protocol.id":"blood_collection","lineage_3":"Donor_SRP_blood_20150602_T_naive","cell_count":"2040000","cell_type":"CD8_T_naive","protocol.id":"cell_extraction","lineage_4":"Donor_SRP_blood_20150602_T_naive_plate7","cell_type":"CD8_T_naive","plate_media_tare":"-1","plate_media_tare%units":"g","protocol.id":"normal_13CGlc","replicate":"1","replicate%type":"biological","lineage_5":"Donor_SRP_blood_20150602_T_naive_plate7-cells","cell_type":"CD8_T_naive","protocol.id":"cell_quench_collection","lineage_6":"Donor_SRP_blood_20150602_T_naive_plate7-cells-polar_GCMS_A","protocol.id":"polar_extraction","replicate":"1","replicate%type":"analytical","type":"polar","weight":"-1","weight%units":"g"} }, { "Subject ID":"-", "Sample ID":"20150602_T_naive_plate8-cells", "Factors":{"cell_type":"CD8_T_naive"}, "Additional sample data":{"protocol.id":"galactose_13CGlc","lineage_1":"Donor_SRP","protocol.id":"normal","species":"Homo sapiens","species_type":"Animal","taxonomy_id":"9606","lineage_2":"Donor_SRP_blood_20150602","protocol.id":"blood_collection","lineage_3":"Donor_SRP_blood_20150602_T_naive","cell_count":"2040000","cell_type":"CD8_T_naive","protocol.id":"cell_extraction","lineage_4":"Donor_SRP_blood_20150602_T_naive_plate8","cell_type":"CD8_T_naive","plate_media_tare":"-1","plate_media_tare%units":"g","protocol.id":"galactose_13CGlc","replicate":"1","replicate%type":"biological","lineage_5":"Donor_SRP_blood_20150602_T_naive_plate8-cells","cell_type":"CD8_T_naive","protocol.id":"cell_quench_collection","lineage_6":"Donor_SRP_blood_20150602_T_naive_plate8-cells-polar_GCMS_A","protocol.id":"polar_extraction","replicate":"1","replicate%type":"analytical","type":"polar","weight":"-1","weight%units":"g"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"Blood collected from a draw. Collection and quench of cells in tissue culture Media is extracted from plate.","COLLECTION_PROTOCOL_ID":"blood_collection cell_quench_collection cells_media","COLLECTION_PROTOCOL_FILENAME":"WS_Fan_Quench_cell_tissue.pdf","SAMPLE_TYPE":"T-Cells"}, "TREATMENT":{"TREATMENT_SUMMARY":"Grown in unlabeled glucose and treated with galactose. Grown in labeled glucose and treated with galactose. Normal human, no treatment. Grown in unlabeled glucose. Grown in labeled glucose.","TREATMENT_PROTOCOL_ID":"galactose_12CGlc galactose_13CGlc normal normal_12CGlc normal_13CGlc"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Cells of interest extracted from the blood. Polar extraction from cells.","SAMPLEPREP_PROTOCOL_ID":"cell_extraction polar_extraction","SAMPLEPREP_PROTOCOL_FILENAME":"WS_Fan_Extract_Polar_Lipid_Prot.pdf"}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Targeted GC","CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"GC-EI-MS, Thermo ITQ","COLUMN_NAME":"SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 µm BPX-5 (5% phenylmethyl- phenylsiloxane) BPX-5 (5% phenylmethyl- phenylsiloxane)"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"INSTRUMENT_NAME":"Thermo ITQ","INSTRUMENT_TYPE":"GC-ITQ","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide)."}, "MS_METABOLITE_DATA":{ "Units":"normalized peak area", 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} }