{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001177","ANALYSIS_ID":"AN001953","VERSION":"1","CREATED_ON":"May 1, 2019, 2:15 pm"},

"PROJECT":{"PROJECT_TITLE":"Short Chain Lipid Project","PROJECT_TYPE":"Untargted analysis","PROJECT_SUMMARY":"Detect phosphatidylcholine (PC) with short acyl chains on the Golgi membrane","INSTITUTE":"Brigham and Women’s Hospital","DEPARTMENT":"Division of Rheumatology, Immunology and Allergy","LAST_NAME":"Hsu","FIRST_NAME":"Victor","ADDRESS":"60 Fenwood Road, Boston, MA 02115 USA","EMAIL":"vhsu@bwh.harvard.edu","PHONE":"617-525-1103"},

"STUDY":{"STUDY_TITLE":"Analysis of short chain phosphatidylcholine (PC) on the Golgi membrane","STUDY_TYPE":"Golgi membrane lipids characterization","STUDY_SUMMARY":"Studies on vesicle formation by the Coat Protein I (COPI) complex have contributed to a basic understanding of how vesicular transport is initiated. We have identified that short chain lipids promote membrane properties that are conducive for fission. Here we investigated short chain PCs on Golgi membrane. These findings will advance the understanding of how lipid geometry contributes to membrane deformation needed for vesicle fission.","INSTITUTE":"University of Kentucky","LAST_NAME":"Morris","FIRST_NAME":"Andrew","ADDRESS":"900 S limestone","EMAIL":"a.j.morris@uky.edu","PHONE":"859-323-3749"},

"SUBJECT":{"SUBJECT_TYPE":"Other","SUBJECT_SPECIES":"Cricetulus griseus","TAXONOMY_ID":"10029"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"20180922_sample_01",
"Factors":{"Sample Type":"Golgi membrane sample"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Golgi membrane were prepared from Chinese hamster ovary cell line as described previously. J Cell Biol 159, 69-78 (2002); EMBO J 24, 4133-4143 (2005).","SAMPLE_TYPE":"CHO cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"Golgi membrane were prepared as described previously. J Cell Biol 159, 69-78 (2002); EMBO J 24, 4133-4143 (2005)."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"5 mg of Golgi membrane fraction was extracted using acidified CHCl3/methanol (1:2)"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Lipid extracts were separated on a Waters ACQUITY BEH C8 column (2.1 × 100 mm, 1.7 μm) with the temperature maintained at 40 °C. The flow rate was 250 μL/min, and the mobile phases were consisted of 60:40 water/acetonitrile (A), and 90:10 isopropanol/acetonitrile (B), both containing 10 mM ammonium formate and 0.1% formic acid. The samples were eluted with a linear gradient from 5 % B to 97 % B over 20 min, maintained at 97 % B for 4 min and re-equilibration with 5 % B for 6 min. The sample injection volume was 5 μL","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","COLUMN_NAME":"ACQUITY BEH C8 column (2.1 × 100 mm, 1.7 µm)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Data analysis and accurate mass calculation were performed using the software Xcalibur 4.0","MS_RESULTS_FILE":"ST001177_AN001953_Results.txt UNITS:Peak area Has m/z:Yes Has RT:Yes RT units:Minutes"}

}