{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001950","ANALYSIS_ID":"AN003175","VERSION":"1","CREATED_ON":"October 24, 2021, 5:18 pm"},

"PROJECT":{"PROJECT_TITLE":"Lipidome Alterations Following Mild Traumatic Brain Injury.","PROJECT_SUMMARY":"Traumatic brain injury (TBI) poses a major health challenge, with tens of millions of new cases reported globally every year. Brain damage resulting from TBI can vary significantly due to factors including injury severity, diffusivity, modality, time delay relative to impact, and exposure to repeated injury events. Untargeted lipidomic analysis of Sprague-Dawley rat serum within 24 hours of mild single and repeat controlled cortical impact (CCI) injury events led to the discovery of biomarker candidates of TBI. Lipid biomarkers have a unique potential to serve as objective molecular measures of the body’s response to injury as their alteration in brain tissue can be more freely observed than for larger protein markers. Animal serum was analyzed via ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) in positive and negative ion modes. Known lipid species were identified through matching to in-house tandem MS databases. Machine learning and feature selection approaches were used to construct lipid panels capable of distinguishing serum from injured and uninjured animals across a range of injury severities and timepoints within the first day of injury. The best multivariate lipid panels had over 90% cross-validated sensitivity, selectivity, and accuracy and consisted of species from nine different lipid classes. These mapped onto sphingolipid signaling, autophagy, necroptosis and glycerophospholipid metabolism pathways, with FDR corrected p-values better than 0.05.","INSTITUTE":"Georgia Institute of Technology","DEPARTMENT":"Chemistry and Biochemistry","LABORATORY":"Facundo Fernández","LAST_NAME":"Gier","FIRST_NAME":"Eric","ADDRESS":"311 Ferst Dr. Atlanta, GA, 30318, USA","EMAIL":"ericgier4@gmail.com","PHONE":"2246221699"},

"STUDY":{"STUDY_TITLE":"Lipidome Alterations Following Mild Traumatic Brain Injury.","STUDY_TYPE":"Untargeted Lipidomics","STUDY_SUMMARY":"Traumatic brain injury (TBI) poses a major health challenge, with tens of millions of new cases reported globally every year. Brain damage resulting from TBI can vary significantly due to factors including injury severity, diffusivity, modality, time delay relative to impact, and exposure to repeated injury events. Untargeted lipidomic analysis of Sprague-Dawley rat serum within 24 hours of mild single and repeat controlled cortical impact (CCI) injury events led to the discovery of biomarker candidates of TBI. Lipid biomarkers have a unique potential to serve as objective molecular measures of the body’s response to injury as their alteration in brain tissue can be more freely observed than for larger protein markers. Animal serum was analyzed via ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) in positive and negative ion modes. Known lipid species were identified through matching to in-house tandem MS databases. Machine learning and feature selection approaches were used to construct lipid panels capable of distinguishing serum from injured and uninjured animals across a range of injury severities and timepoints within the first day of injury. The best multivariate lipid panels had over 90% cross-validated sensitivity, selectivity, and accuracy and consisted of species from nine different lipid classes. These mapped onto sphingolipid signaling, autophagy, necroptosis and glycerophospholipid metabolism pathways, with FDR corrected p-values better than 0.05.","INSTITUTE":"Georgia Institute of Technology","DEPARTMENT":"Chemistry and Biochemistry","LABORATORY":"Facundo Fernández","LAST_NAME":"Gier","FIRST_NAME":"Eric","ADDRESS":"311 Ferst Drive, Atlanta, GA, 30318, USA","EMAIL":"egier3@gatech.edu","PHONE":"2246221699","NUM_GROUPS":"6","TOTAL_SUBJECTS":"32","NUM_MALES":"14","NUM_FEMALES":"18","STUDY_COMMENTS":"LC-MS"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Rattus norvegicus","TAXONOMY_ID":"10116","GENOTYPE_STRAIN":"Sprague-Dawley","AGE_OR_AGE_RANGE":"8 weeks","WEIGHT_OR_WEIGHT_RANGE":"250-450 g","GENDER":"Male and female","ANIMAL_ANIMAL_SUPPLIER":"Charles River","ANIMAL_LIGHT_CYCLE":"12 h reverse light-dark cycles","ANIMAL_FEED":"ad libitum","ANIMAL_WATER":"ad libitum"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"1",
"Sample ID":"1C_T3_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"1C_T3_I1_G2_L1.mzML"}
},
{
"Subject ID":"1",
"Sample ID":"1D_T4_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"1D_T4_I1_G2_L1.mzML"}
},
{
"Subject ID":"2",
"Sample ID":"2A_T1_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"2A_T1_I1_G2_L1.mzML"}
},
{
"Subject ID":"2",
"Sample ID":"2B_T2_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"2B_T2_I1_G2_L1.mzML"}
},
{
"Subject ID":"2",
"Sample ID":"2C_T3_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"2C_T3_I1_G2_L1.mzML"}
},
{
"Subject ID":"2",
"Sample ID":"2D_T4_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"2D_T4_I1_G2_L1.mzML"}
},
{
"Subject ID":"3",
"Sample ID":"3A_T1_I2_G2_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"M","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"3A_T1_I2_G2_L1.mzML"}
},
{
"Subject ID":"3",
"Sample ID":"3C_T3_I2_G2_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"M","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"3C_T3_I2_G2_L1.mzML"}
},
{
"Subject ID":"3",
"Sample ID":"3D_T4_I2_G2_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"M","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"3D_T4_I2_G2_L1.mzML"}
},
{
"Subject ID":"4",
"Sample ID":"4A_T1_I3_G2_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"M","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"4A_T1_I3_G2_L1.mzML"}
},
{
"Subject ID":"4",
"Sample ID":"4C_T3_I3_G2_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"M","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"4C_T3_I3_G2_L1.mzML"}
},
{
"Subject ID":"4",
"Sample ID":"4D_T4_I3_G2_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"M","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"4D_T4_I3_G2_L1.mzML"}
},
{
"Subject ID":"5",
"Sample ID":"5A_T1_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"5A_T1_I1_G2_L1.mzML"}
},
{
"Subject ID":"5",
"Sample ID":"5B_T2_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"5B_T2_I1_G2_L1.mzML"}
},
{
"Subject ID":"5",
"Sample ID":"5C_T3_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"5C_T3_I1_G2_L1.mzML"}
},
{
"Subject ID":"5",
"Sample ID":"5D_T4_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"5D_T4_I1_G2_L1.mzML"}
},
{
"Subject ID":"6",
"Sample ID":"6A_T1_I2_G2_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"M","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"6A_T1_I2_G2_L1.mzML"}
},
{
"Subject ID":"6",
"Sample ID":"6B_T2_I2_G2_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"M","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"6B_T2_I2_G2_L1.mzML"}
},
{
"Subject ID":"6",
"Sample ID":"6C_T3_I2_G2_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"M","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"6C_T3_I2_G2_L1.mzML"}
},
{
"Subject ID":"6",
"Sample ID":"6D_T4_I2_G2_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"M","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"6D_T4_I2_G2_L1.mzML"}
},
{
"Subject ID":"7",
"Sample ID":"7A_T1_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"7A_T1_I1_G2_L1.mzML"}
},
{
"Subject ID":"7",
"Sample ID":"7B_T2_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"7B_T2_I1_G2_L1.mzML"}
},
{
"Subject ID":"7",
"Sample ID":"7C_T3_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"7C_T3_I1_G2_L1.mzML"}
},
{
"Subject ID":"7",
"Sample ID":"7D_T4_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"7D_T4_I1_G2_L1.mzML"}
},
{
"Subject ID":"8",
"Sample ID":"8A_T1_I2_G2_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"M","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"8A_T1_I2_G2_L1.mzML"}
},
{
"Subject ID":"8",
"Sample ID":"8B_T2_I2_G2_L2",
"Factors":{"Injury Severity":"Single-Impact","Sex":"M","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"8B_T2_I2_G2_L2.mzML"}
},
{
"Subject ID":"8",
"Sample ID":"8C_T3_I2_G2_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"M","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"8C_T3_I2_G2_L1.mzML"}
},
{
"Subject ID":"8",
"Sample ID":"8D_T4_I2_G2_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"M","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"8D_T4_I2_G2_L1.mzML"}
},
{
"Subject ID":"9",
"Sample ID":"9A_T1_I3_G2_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"M","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"9A_T1_I3_G2_L1.mzML"}
},
{
"Subject ID":"9",
"Sample ID":"9C_T3_I3_G2_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"M","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"9C_T3_I3_G2_L1.mzML"}
},
{
"Subject ID":"9",
"Sample ID":"9D_T4_I3_G2_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"M","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"9D_T4_I3_G2_L1.mzML"}
},
{
"Subject ID":"10",
"Sample ID":"10A_T1_I2_G2_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"M","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"10A_T1_I2_G2_L1.mzML"}
},
{
"Subject ID":"10",
"Sample ID":"10C_T3_I2_G2_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"M","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"10C_T3_I2_G2_L1.mzML"}
},
{
"Subject ID":"10",
"Sample ID":"10D_T4_I2_G2_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"M","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"10D_T4_I2_G2_L1.mzML"}
},
{
"Subject ID":"11",
"Sample ID":"11A_T1_I3_G2_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"M","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"11A_T1_I3_G2_L1.mzML"}
},
{
"Subject ID":"11",
"Sample ID":"11B_T2_I3_G2_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"M","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"11B_T2_I3_G2_L1.mzML"}
},
{
"Subject ID":"11",
"Sample ID":"11C_T3_I3_G2_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"M","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"11C_T3_I3_G2_L1.mzML"}
},
{
"Subject ID":"11",
"Sample ID":"11D_T4_I3_G2_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"M","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"11D_T4_I3_G2_L1.mzML"}
},
{
"Subject ID":"12",
"Sample ID":"12A_T1_I3_G2_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"M","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"12A_T1_I3_G2_L1.mzML"}
},
{
"Subject ID":"12",
"Sample ID":"12B_T2_I3_G2_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"M","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"12B_T2_I3_G2_L1.mzML"}
},
{
"Subject ID":"12",
"Sample ID":"12C_T3_I3_G2_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"M","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"12C_T3_I3_G2_L1.mzML"}
},
{
"Subject ID":"12",
"Sample ID":"12D_T4_I3_G2_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"M","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"12D_T4_I3_G2_L1.mzML"}
},
{
"Subject ID":"13",
"Sample ID":"13A_T1_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"13A_T1_I2_G1_L1.mzML"}
},
{
"Subject ID":"13",
"Sample ID":"13B_T2_I2_G1_L2",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"13B_T2_I2_G1_L2.mzML"}
},
{
"Subject ID":"13",
"Sample ID":"13C_T3_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"13C_T3_I2_G1_L1.mzML"}
},
{
"Subject ID":"14",
"Sample ID":"14A_T1_I1_G1_L1",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"14A_T1_I1_G1_L1.mzML"}
},
{
"Subject ID":"14",
"Sample ID":"14B_T2_I1_G1_L1",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"14B_T2_I1_G1_L1.mzML"}
},
{
"Subject ID":"14",
"Sample ID":"14C_T3_I1_G1_L1",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"14C_T3_I1_G1_L1.mzML"}
},
{
"Subject ID":"14",
"Sample ID":"14D_T4_I1_G1_L1",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"14D_T4_I1_G1_L1.mzML"}
},
{
"Subject ID":"15",
"Sample ID":"15A_T1_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"15A_T1_I2_G1_L1.mzML"}
},
{
"Subject ID":"15",
"Sample ID":"15B_T2_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"15B_T2_I2_G1_L1.mzML"}
},
{
"Subject ID":"15",
"Sample ID":"15C_T3_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"15C_T3_I2_G1_L1.mzML"}
},
{
"Subject ID":"16",
"Sample ID":"16A_T1_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"16A_T1_I3_G1_L1.mzML"}
},
{
"Subject ID":"16",
"Sample ID":"16B_T2_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"16B_T2_I3_G1_L1.mzML"}
},
{
"Subject ID":"16",
"Sample ID":"16C_T3_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"16C_T3_I3_G1_L1.mzML"}
},
{
"Subject ID":"17",
"Sample ID":"17A_T1_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"17A_T1_I2_G1_L1.mzML"}
},
{
"Subject ID":"17",
"Sample ID":"17B_T2_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"17B_T2_I2_G1_L1.mzML"}
},
{
"Subject ID":"17",
"Sample ID":"17C_T3_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"17C_T3_I2_G1_L1.mzML"}
},
{
"Subject ID":"17",
"Sample ID":"17D_T4_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"17D_T4_I2_G1_L1.mzML"}
},
{
"Subject ID":"18",
"Sample ID":"18A_T1_I1_G1_L1",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"18A_T1_I1_G1_L1.mzML"}
},
{
"Subject ID":"18",
"Sample ID":"18B_T2_I1_G1_L1",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"18B_T2_I1_G1_L1.mzML"}
},
{
"Subject ID":"18",
"Sample ID":"18C_T3_I1_G1_L1",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"18C_T3_I1_G1_L1.mzML"}
},
{
"Subject ID":"18",
"Sample ID":"18D_T4_I1_G1_L1",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"18D_T4_I1_G1_L1.mzML"}
},
{
"Subject ID":"19",
"Sample ID":"19B_T2_I1_G1_L2",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"19B_T2_I1_G1_L2.mzML"}
},
{
"Subject ID":"19",
"Sample ID":"19C_T3_I1_G1_L1",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"19C_T3_I1_G1_L1.mzML"}
},
{
"Subject ID":"19",
"Sample ID":"19D_T4_I1_G1_L1",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"19D_T4_I1_G1_L1.mzML"}
},
{
"Subject ID":"20",
"Sample ID":"20A_T1_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"20A_T1_I3_G1_L1.mzML"}
},
{
"Subject ID":"20",
"Sample ID":"20B_T2_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"20B_T2_I3_G1_L1.mzML"}
},
{
"Subject ID":"20",
"Sample ID":"20C_T3_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"20C_T3_I3_G1_L1.mzML"}
},
{
"Subject ID":"20",
"Sample ID":"20D_T4_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"20D_T4_I3_G1_L1.mzML"}
},
{
"Subject ID":"21",
"Sample ID":"21C_T3_I1_G1_L2",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"21C_T3_I1_G1_L2.mzML"}
},
{
"Subject ID":"21",
"Sample ID":"21D_T4_I1_G1_L1",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"21D_T4_I1_G1_L1.mzML"}
},
{
"Subject ID":"22",
"Sample ID":"22A_T1_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"22A_T1_I2_G1_L1.mzML"}
},
{
"Subject ID":"22",
"Sample ID":"22C_T3_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"22C_T3_I2_G1_L1.mzML"}
},
{
"Subject ID":"22",
"Sample ID":"22D_T4_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"22D_T4_I2_G1_L1.mzML"}
},
{
"Subject ID":"23",
"Sample ID":"23A_T1_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"23A_T1_I3_G1_L1.mzML"}
},
{
"Subject ID":"23",
"Sample ID":"23B_T2_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"23B_T2_I3_G1_L1.mzML"}
},
{
"Subject ID":"23",
"Sample ID":"23C_T3_I3_G1_L2",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"23C_T3_I3_G1_L2.mzML"}
},
{
"Subject ID":"23",
"Sample ID":"23D_T4_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"23D_T4_I3_G1_L1.mzML"}
},
{
"Subject ID":"24",
"Sample ID":"24A_T1_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"24A_T1_I3_G1_L1.mzML"}
},
{
"Subject ID":"24",
"Sample ID":"24B_T2_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"24B_T2_I3_G1_L1.mzML"}
},
{
"Subject ID":"24",
"Sample ID":"24C_T3_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"24C_T3_I3_G1_L1.mzML"}
},
{
"Subject ID":"25",
"Sample ID":"25A_T1_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"25A_T1_I1_G2_L1.mzML"}
},
{
"Subject ID":"25",
"Sample ID":"25B_T2_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"25B_T2_I1_G2_L1.mzML"}
},
{
"Subject ID":"25",
"Sample ID":"25C_T3_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"25C_T3_I1_G2_L1.mzML"}
},
{
"Subject ID":"25",
"Sample ID":"25D_T4_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"25D_T4_I1_G2_L1.mzML"}
},
{
"Subject ID":"26",
"Sample ID":"26A_T1_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"26A_T1_I1_G2_L1.mzML"}
},
{
"Subject ID":"26",
"Sample ID":"26B_T2_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"26B_T2_I1_G2_L1.mzML"}
},
{
"Subject ID":"26",
"Sample ID":"26C_T3_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"26C_T3_I1_G2_L1.mzML"}
},
{
"Subject ID":"26",
"Sample ID":"26D_T4_I1_G2_L1",
"Factors":{"Injury Severity":"Sham","Sex":"M","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"26D_T4_I1_G2_L1.mzML"}
},
{
"Subject ID":"27",
"Sample ID":"27A_T1_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"27A_T1_I2_G1_L1.mzML"}
},
{
"Subject ID":"27",
"Sample ID":"27B_T2_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"27B_T2_I2_G1_L1.mzML"}
},
{
"Subject ID":"27",
"Sample ID":"27C_T3_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"27C_T3_I2_G1_L1.mzML"}
},
{
"Subject ID":"27",
"Sample ID":"27D_T4_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"27D_T4_I2_G1_L1.mzML"}
},
{
"Subject ID":"28",
"Sample ID":"28A_T1_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"28A_T1_I3_G1_L1.mzML"}
},
{
"Subject ID":"28",
"Sample ID":"28B_T2_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"28B_T2_I3_G1_L1.mzML"}
},
{
"Subject ID":"28",
"Sample ID":"28C_T3_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"28C_T3_I3_G1_L1.mzML"}
},
{
"Subject ID":"28",
"Sample ID":"28D_T4_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"28D_T4_I3_G1_L1.mzML"}
},
{
"Subject ID":"29",
"Sample ID":"29A_T1_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"29A_T1_I2_G1_L1.mzML"}
},
{
"Subject ID":"29",
"Sample ID":"29B_T2_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"29B_T2_I2_G1_L1.mzML"}
},
{
"Subject ID":"29",
"Sample ID":"29C_T3_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"29C_T3_I2_G1_L1.mzML"}
},
{
"Subject ID":"29",
"Sample ID":"29D_T4_I2_G1_L1",
"Factors":{"Injury Severity":"Single-Impact","Sex":"F","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"29D_T4_I2_G1_L1.mzML"}
},
{
"Subject ID":"30",
"Sample ID":"30A_T1_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"30A_T1_I3_G1_L1.mzML"}
},
{
"Subject ID":"30",
"Sample ID":"30B_T2_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"30B_T2_I3_G1_L1.mzML"}
},
{
"Subject ID":"30",
"Sample ID":"30C_T3_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"30C_T3_I3_G1_L1.mzML"}
},
{
"Subject ID":"30",
"Sample ID":"30D_T4_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"30D_T4_I3_G1_L1.mzML"}
},
{
"Subject ID":"31",
"Sample ID":"31A_T1_I1_G1_L1",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"31A_T1_I1_G1_L1.mzML"}
},
{
"Subject ID":"31",
"Sample ID":"31B_T2_I1_G1_L1",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"31B_T2_I1_G1_L1.mzML"}
},
{
"Subject ID":"31",
"Sample ID":"31C_T3_I1_G1_L1",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"31C_T3_I1_G1_L1.mzML"}
},
{
"Subject ID":"31",
"Sample ID":"31D_T4_I1_G1_L1",
"Factors":{"Injury Severity":"Sham","Sex":"F","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"31D_T4_I1_G1_L1.mzML"}
},
{
"Subject ID":"32",
"Sample ID":"32A_T1_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"Baseline"},
"Additional sample data":{"RAW_FILE_NAME":"32A_T1_I3_G1_L1.mzML"}
},
{
"Subject ID":"32",
"Sample ID":"32B_T2_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"30 min"},
"Additional sample data":{"RAW_FILE_NAME":"32B_T2_I3_G1_L1.mzML"}
},
{
"Subject ID":"32",
"Sample ID":"32C_T3_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"4 hour"},
"Additional sample data":{"RAW_FILE_NAME":"32C_T3_I3_G1_L1.mzML"}
},
{
"Subject ID":"32",
"Sample ID":"32D_T4_I3_G1_L1",
"Factors":{"Injury Severity":"Repeat-Impact","Sex":"F","Blood Collection":"24 hour"},
"Additional sample data":{"RAW_FILE_NAME":"32D_T4_I3_G1_L1.mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Approximately 200 µL of whole blood was collected from a tail vein punctured by 20-gauge Precision Glide needles and stored on ice. Whole blood samples were allowed to coagulate at room temperature for 45 minutes. Samples were then centrifuged at 4 °C for 15 min at 2500 x g, and serum was collected in 50 μL aliquots and stored at -80 °C.","COLLECTION_PROTOCOL_ID":"A100188","COLLECTION_PROTOCOL_COMMENTS":"All procedures involving Sprague-Dawley rat models were performed in accordance with guidelines set forth in the Guide for the Care and Use of Laboratory Animals (U.S. Department of Health and Human Services, Pub no. 85-23, 1985) and were approved by the Georgia Institute of Technology Institutional Animal Care and Use Committee","SAMPLE_TYPE":"Blood (serum)","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"A CCI device (Pittsburgh Precision Instruments, Pittsburgh, PA) was used to induce single and repetitive closed-head injuries to the cortex. Prior to injuries, all rat groups were anesthetized with isoflurane (induction: 5% isoflurane; maintenance: 2-3% isoflurane) and a toe pinch was administered to evaluate loss of consciousness and ensure minimal pain during injury. A pneumatic piston on the CCI device with a 5 mm tip diameter was positioned 15 degrees below the vertical axis of the coronal plane to induce injury to the closed skull, 30 s after removal of the isoflurane supply. All injury groups received impacts from the pneumatic piston at a velocity of 5 m/s. The single impact injury group received one injury with a 5 mm head displacement. The repeat injury group received a total of 3 injuries at 2 min intervals, with head displacements of 5 mm, 2 mm, and 2 mm, respectively. Sham-operated animals received a treatment identical to injured animals but excluding the injury procedure. Following final injury, time-to-right was recorded, and animals were monitored to survey the presence of neurological deficits. Animals were returned to home cages and singly housed with soft bedding during recovery."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"A standard spiked IPA solution was prepared with 250 µL of SPLASH II Lipidomix and 14.750 mL of IPA. Serum samples were thawed on ice for one hour prior to the addition of the IPA solution in a 1:3 v/v ratio to separate lipids and small non-polar metabolites from proteins. Mixtures of serum and IPA solution were vortexed for 10 s and centrifuged at 16000g for 7 min. The supernatant was then collected for LC-MS analysis. Sample blanks were prepared with 50 µL of LC-MS grade water, and pooled QC samples were prepared from 5 µL aliquots of all study subject serum samples. Serum reference samples from uninjured Sprague-Dawley rat serum were processed in the same manner as study subject serum samples."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"All samples were run in a randomized order over two and a half days of consecutive instrument time. QC samples were interleaved every 24 runs to evaluate LC-MS system stability and to account for time-dependent batch effects.","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Thermo Accucore C30 (2.1 x 50 mm, 2.1um)","FLOW_GRADIENT":"(Time: A/B) 0 min 80/20, 1 min 40/60, 5 min 30/70, 5.5 min 15/85, 8 min 10/90, 8.2 min 0/100, 10.7 min 80/20, 12 min 80/20","FLOW_RATE":"0.3 mL/min","COLUMN_TEMPERATURE":"60 ℃","SOLVENT_A":"(40:60 v/v) water/ACN mixture with 10 mM acetonitrile and 0.1% formic acid","SOLVENT_B":"(90:10 v/v) IPA/ACN mixture with 10 mM acetonitrile and 0.1% formic acid"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"Fernández","DETECTOR_TYPE":"Orbitrap"},

"MS":{"INSTRUMENT_NAME":"Thermo ID-X Orbitrap Tribrid","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"MS experiments were preformed over a scan range of 150-2000 m/z, maximum ion injection time was set to 200 ms, and orbitrap resolution was 24000. Raw spectral data from LC-MS experiments were pre-processed using Compound Discoverer v3.0.0 software (Thermo Fischer Scientific, Inc., Waltham, MA) and the XCMS web-based application (xcmsonline.scripps.edu). Initial steps involved retention time alignment between samples, peak area integration, peak picking, and QC area normalization. Features eluting with the solvent front or having retention times below 0.75 min were removed to account for potential ion suppression effects in that retention time region. ChemSpider and in-house mzVault database searches were used to obtain a list of tentative IDs based on accurate mass, isotope pattern, and MS/MS data whenever possible. Each lipid feature was identified according to the following confidence levels: (1) compounds matched to existing in house database standards by accurate mass (<2 ppm), isotopic abundance, fragmentation spectrum, and retention time; (2) compounds annotated according to accurate mass, isotopic abundance, and fragmentation consistent with Lipid Maps and Human Metabolome Database (HMDB) entries; (3) accurate mass match matched to Lipid Maps and HMDB entries and fragmentation showing a few matching characteristic fragment ions.","ION_SOURCE_TEMPERATURE":"275 ℃","ION_SPRAY_VOLTAGE":"-2500 V","SOURCE_TEMPERATURE":"320 ℃"},

"MS_METABOLITE_DATA":{
"Units":"Normalized Peak Area",

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or PC(P-18:0)"},{"metabolite_name":"PC(O-26:1)"},{"metabolite_name":"PC(O-32:0)>PC(O-16:0/16:0)"},{"metabolite_name":"PC(O-32:1)>PC(O-16:1/16:0)"},{"metabolite_name":"PC(O-34:1)>PC(O-18:1/16:0)"},{"metabolite_name":"PC(O-34:3) or PC(P-34:2)"},{"metabolite_name":"PC(O-36:1)>PC(O-18:0/18:1)"},{"metabolite_name":"PC(O-36:1)>PC(O-18:1/18:0)_and_PC(O-16:0/20:1)"},{"metabolite_name":"PC(O-36:4)>PC(O-16:0/20:4)"},{"metabolite_name":"PC(O-36:5)>PC(O-16:1/20:4)"},{"metabolite_name":"PC(O-38:1) or PC(P-38:0)"},{"metabolite_name":"PC(O-38:4)>PC(O-16:0/22:4)"},{"metabolite_name":"PC(O-38:4)>PC(O-18:0/20:4)"},{"metabolite_name":"PC(O-38:6)>PC(O-16:0/22:6)"},{"metabolite_name":"PC(O-38:6)>PC(O-18:2/20:4)"},{"metabolite_name":"PC(O-38:7)>PC(O-16:2/22:5)"},{"metabolite_name":"PC(O-40:4)>PC(O-20:0/20:4)"},{"metabolite_name":"PC(O-40:5) or PC(P-40:4)"},{"metabolite_name":"PC(O-40:9)>PC(O:20:5/20:4)"},{"metabolite_name":"PC(P-38:5)>PC(P-18:1/20:4)"},{"metabolite_name":"PE(34:1)>PE(16:0_18:1)"},{"metabolite_name":"PE(34:2)>PE(16:0_18:2)"},{"metabolite_name":"PE(36:1)>PE(18:0_18:1)"},{"metabolite_name":"PE(36:2)>PE(18:0_18:2)"},{"metabolite_name":"PE(36:3)>PE(18:1_18:2)"},{"metabolite_name":"PE(36:4)>(16:0_20:4)"},{"metabolite_name":"PE(36:5)>PE(16:0_20:5)"},{"metabolite_name":"PE(38:4)>(PE(18:0_20:4)"},{"metabolite_name":"PE(38:4)>PE(18:0_20:4)"},{"metabolite_name":"PE(38:5)>PE(18:0_20:5)"},{"metabolite_name":"PE(38:6)>PE(18:2_20:4)"},{"metabolite_name":"PE(40:6)>PE(18:0_22:6)"},{"metabolite_name":"PE(40:7)>PE(18:1_22:6)_and_PE(18:0_22:7)"},{"metabolite_name":"PE(O-34:2)>PE(O-16:2/18:0)_and_PE(O-18:2/16:0)"},{"metabolite_name":"PE(O-34:5)>PE(O-17:1/20:4)"},{"metabolite_name":"PE(O-36:4)>PE(O-16:0/20:4)"},{"metabolite_name":"PE(O-36:5)>PE(O-16:1/20:4)"},{"metabolite_name":"PE(O-38:2)>PE(O-20:0/18:2)"},{"metabolite_name":"PE(O-38:4)>PE(O-16:0/22:4)"},{"metabolite_name":"PE(O-38:4)>PE(O-18:0/20:4)_and_PE(O-18:2/20:2)"},{"metabolite_name":"PE(O-38:5)>PE(O-18:1/20:4)"},{"metabolite_name":"PE(O-38:6)>PE(O-18:2/20:4)_and_PE(O-16:1/22:5)"},{"metabolite_name":"PE(O-38:7)>PE(O-16:1/22:6)_and_PE(O-20:7/18:0)"},{"metabolite_name":"PE(O-38:7)>PE(O-20:7/18:0)_and_PE(O-16:1/22:6)"},{"metabolite_name":"PE(O-40:4)>PE(O-18:0/22:4)"},{"metabolite_name":"PE(O-40:5)>PE(O-18:1/22:4)"},{"metabolite_name":"PE(O-40:6)>PE(O-18:1/22:5)"},{"metabolite_name":"PE(O-40:6)>PE(O-18:2/22:4)"},{"metabolite_name":"PE(O-40:7)>PE(O-18:1/22:6)_and_PE(O-22:6/18:1)"},{"metabolite_name":"PE(O-40:7)>PE(O-18:1/22:6)_and_PE(O-22:7/18:0)"},{"metabolite_name":"PE(O-40:7)>PE(O-18:2/22:5)"},{"metabolite_name":"PE(O-40:8)>PE(O-22:8/18:0)_and_PE(O-18:2/22:6)"},{"metabolite_name":"PE(O-42:5)>PE(O-20:1/22:4)"},{"metabolite_name":"PE(O-42:7)>PE(O-20:1/22:6)"},{"metabolite_name":"PE(P-40:3)>PE(P-18:0/22:3)"},{"metabolite_name":"PE(P-40:4)>PE(P-20:0/20:4)"},{"metabolite_name":"PI(34:1)"},{"metabolite_name":"PI(34:2)>PI(16:0_18:2)"},{"metabolite_name":"PI(36:1)>PI(18:0_18:1)"},{"metabolite_name":"PI(36:2)>PI(18:0_18:2)"},{"metabolite_name":"PI(36:2)>PI(18:1_18:1)"},{"metabolite_name":"PI(36:3)>PI(18:1_18:2)"},{"metabolite_name":"PI(36:4)>PI(16:0_20:4)"},{"metabolite_name":"PI(38:3)>PI(18:0_20:3)"},{"metabolite_name":"PI(38:4)>PI(18:0_20:4)"},{"metabolite_name":"PI(38:5)>PI(18:1_20:4)"},{"metabolite_name":"PI(40:4)>PI(18:0_22:4)"},{"metabolite_name":"PI(40:5)>PI(18:0_22:5)"},{"metabolite_name":"PI(40:6)>PI(18:0_22:6)"},{"metabolite_name":"PS(36:1)>PS(18:0_18:1)"},{"metabolite_name":"PS(38:4)>PS(18:0_20:4)"},{"metabolite_name":"PS(38:5)"},{"metabolite_name":"PS(40:0)"},{"metabolite_name":"PS(40:6)>PS(18:0_22:6)"},{"metabolite_name":"PS(40:8)>PS(20:4/20:4)"},{"metabolite_name":"PS(O-42:7)>PS(O-20:3/22:4)"},{"metabolite_name":"SM(d34:0)"},{"metabolite_name":"SM(d34:1)"},{"metabolite_name":"SM(d34:2)"},{"metabolite_name":"SM(d35:1)"},{"metabolite_name":"SM(d36:1)"},{"metabolite_name":"SM(d36:2)"},{"metabolite_name":"SM(d38:1)"},{"metabolite_name":"SM(d38:2)"},{"metabolite_name":"SM(d39:1)"},{"metabolite_name":"SM(d40:0-OH)"},{"metabolite_name":"SM(d40:1)"},{"metabolite_name":"SM(d40:2)"},{"metabolite_name":"SM(d41:1)"},{"metabolite_name":"SM(d41:2)"},{"metabolite_name":"SM(d42:1)"},{"metabolite_name":"SM(d42:2)"},{"metabolite_name":"SM(d42:3)"},{"metabolite_name":"SM(d42:5)"},{"metabolite_name":"SM(d43:1)"},{"metabolite_name":"SM(d44:1)"},{"metabolite_name":"SM(d44:2)"}]
}

}