#METABOLOMICS WORKBENCH riverup_20230405_193420 DATATRACK_ID:3845 STUDY_ID:ST002541 ANALYSIS_ID:AN004187 PROJECT_ID:PR001637 VERSION 1 CREATED_ON April 6, 2023, 11:09 am #PROJECT PR:PROJECT_TITLE The metabolomics analysis of Methionine restriction PR:PROJECT_TYPE targeted metabolomics data PR:PROJECT_SUMMARY Methionine restriction (MR) provides metabolic benefits in many organisms. PR:PROJECT_SUMMARY However, mechanisms underlying the MR-induced effect remain incompletely PR:PROJECT_SUMMARY understood. Here, we show in the budding yeast S. cerevisiae that MR relays a PR:PROJECT_SUMMARY signal of S-adenosylmethionine (SAM) deprivation to adapt bioenergetic PR:PROJECT_SUMMARY mitochondria to nitrogenic anabolism.In particular, decreases in cellular SAM PR:PROJECT_SUMMARY constrain lipoate metabolism and protein lipoylation required for the operation PR:PROJECT_SUMMARY of the tricarboxylic acid (TCA) cycle in the mitochondria, leading to incomplete PR:PROJECT_SUMMARY glucose oxidation with an exit of acetyl-CoA and -ketoglutarate from the TCA PR:PROJECT_SUMMARY cycle to the syntheses of amino acids, such as arginine and leucine. This PR:PROJECT_SUMMARY mitochondrial SAM-induced response, namely mitoSIR, promotes cell fitness PR:PROJECT_SUMMARY through the coordination of mitochondrial fuel metabolism with the nitrogenic PR:PROJECT_SUMMARY synthesis of amino acids. PR:INSTITUTE Life Sciences Institute, ZheJiang University PR:LAST_NAME Cunqi PR:FIRST_NAME Ye PR:ADDRESS 866 Yuhangtang Rd, Hangzhou 310058, P.R. China PR:EMAIL yecunqi@zju.edu.cn PR:PHONE 15267181160 #STUDY ST:STUDY_TITLE Methionine restriction constrains lipoylation and activates mitochondria for ST:STUDY_TITLE nitrogenic synthesis of amino acids (Part 1) ST:STUDY_SUMMARY Methionine restriction (MR) provides metabolic benefits in many organisms. ST:STUDY_SUMMARY However, mechanisms underlying the MR-induced effect remain incompletely ST:STUDY_SUMMARY understood. Here, we show in the budding yeast S. cerevisiae that MR relays a ST:STUDY_SUMMARY signal of S-adenosylmethionine (SAM) deprivation to adapt bioenergetic ST:STUDY_SUMMARY mitochondria to nitrogenic anabolism. In particular, decreases in cellular SAM ST:STUDY_SUMMARY constrain lipoate metabolism and protein lipoylation required for the operation ST:STUDY_SUMMARY of the tricarboxylic acid (TCA) cycle in the mitochondria, leading to incomplete ST:STUDY_SUMMARY glucose oxidation with an exit of acetyl-CoA and -ketoglutarate from the TCA ST:STUDY_SUMMARY cycle to the syntheses of amino acids, such as arginine and leucine. This ST:STUDY_SUMMARY mitochondrial SAM-induced response, namely mitoSIR, promotes cell fitness ST:STUDY_SUMMARY through the coordination of mitochondrial fuel metabolism with the nitrogenic ST:STUDY_SUMMARY synthesis of amino acids. ST:INSTITUTE Life Sciences Institute, ZheJiang University ST:LAST_NAME Cunqi ST:FIRST_NAME Ye ST:ADDRESS 866 Yuhangtang Rd, Hangzhou 310058, P.R. China ST:EMAIL yecunqi@zju.edu.cn ST:PHONE 15267181160 #SUBJECT SU:SUBJECT_TYPE Yeast SU:SUBJECT_SPECIES Saccharomyces cerevisiae SU:TAXONOMY_ID 4932 SU:GENOTYPE_STRAIN CEN.P.K #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - met6KO_0h_1 Sample Type:0h RAW_FILE_NAME=met6KO_0h_1.wiff SUBJECT_SAMPLE_FACTORS - met6KO_0h_2 Sample Type:0h RAW_FILE_NAME=met6KO_0h_2.wiff SUBJECT_SAMPLE_FACTORS - met6KO_0h_3 Sample Type:0h RAW_FILE_NAME=met6KO_0h_3.wiff SUBJECT_SAMPLE_FACTORS - met6KO_2h_1 Sample Type:2h RAW_FILE_NAME=met6KO_2h_1.wiff SUBJECT_SAMPLE_FACTORS - met6KO_2h_2 Sample Type:2h RAW_FILE_NAME=met6KO_2h_2.wiff SUBJECT_SAMPLE_FACTORS - met6KO_2h_3 Sample Type:2h RAW_FILE_NAME=met6KO_2h_3.wiff SUBJECT_SAMPLE_FACTORS - met6KO_4h_1 Sample Type:4h RAW_FILE_NAME=met6KO_4h_1.wiff SUBJECT_SAMPLE_FACTORS - met6KO_4h_2 Sample Type:4h RAW_FILE_NAME=met6KO_4h_2.wiff SUBJECT_SAMPLE_FACTORS - met6KO_4h_3 Sample Type:4h RAW_FILE_NAME=met6KO_4h_3.wiff SUBJECT_SAMPLE_FACTORS - met6KO_6h_1 Sample Type:6h RAW_FILE_NAME=met6KO_6h_1.wiff SUBJECT_SAMPLE_FACTORS - met6KO_6h_2 Sample Type:6h RAW_FILE_NAME=met6KO_6h_2.wiff SUBJECT_SAMPLE_FACTORS - met6KO_6h_3 Sample Type:6h RAW_FILE_NAME=met6KO_6h_3.wiff SUBJECT_SAMPLE_FACTORS - met6KO_15h_1 Sample Type:15h RAW_FILE_NAME=met6KO_15h_1.wiff SUBJECT_SAMPLE_FACTORS - met6KO_15h_2 Sample Type:15h RAW_FILE_NAME=met6KO_15h_2.wiff SUBJECT_SAMPLE_FACTORS - met6KO_15h_3 Sample Type:15h RAW_FILE_NAME=met6KO_15h_3.wiff #COLLECTION CO:COLLECTION_SUMMARY Care was taken to quench cells quickly in -40℃ and maintain metabolites in CO:COLLECTION_SUMMARY acid to minimize oxidation CO:SAMPLE_TYPE Yeast cells #TREATMENT TR:TREATMENT_SUMMARY Mutant MET6KO cells were cultured in SD medium with the supplements of Met and TR:TREATMENT_SUMMARY growed to log phase, then cells were washed with normal SD medium and TR:TREATMENT_SUMMARY re-cultured in SD medium #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Briefly, equal OD units of cells were rapidly quenched to stop metabolism by SP:SAMPLEPREP_SUMMARY addition into 4 volumes of quenching buffer containing 60% methanol and 10 mM SP:SAMPLEPREP_SUMMARY Tricine, pH 7.4 that was pre-cooled to -40°C. 5 min after holding at -40°C, SP:SAMPLEPREP_SUMMARY cells were spun at 5,000 g for 3 min at 0°C, washed with the same buffer, and SP:SAMPLEPREP_SUMMARY then resuspended in 1 mL extraction buffer containing 75% ethanol and 0.5 mM SP:SAMPLEPREP_SUMMARY Tricine, pH 7.4. Intracellular metabolites were extracted by incubating at 75°C SP:SAMPLEPREP_SUMMARY for 3 min, followed by chilling on ice for 5 min. Samples were spun at 20,000 g SP:SAMPLEPREP_SUMMARY for 1 min to pellet cell debris, and 0.9 mL of the supernatant was transferred SP:SAMPLEPREP_SUMMARY to a new tube. After a second spin at 20,000 g for 10 min, 0.8 mL of the SP:SAMPLEPREP_SUMMARY supernatant was transferred to a new tube. Metabolites in the extraction buffer SP:SAMPLEPREP_SUMMARY were dried using a vacuum concentrator system (Labconco) and stored at -80°C SP:SAMPLEPREP_SUMMARY until analysis. Dried metabolite extracts were resuspended in 60% acetonitrile SP:SAMPLEPREP_SUMMARY for injection. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Low pH polar CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Shimadzu 20AD CH:COLUMN_NAME Merck SeQuant ZIC-pHILIC (150 x 2.1mm,5um) CH:SOLVENT_A 20 mM ammonium carbonate and 0.1% (v/v) ammonium hydroxide CH:SOLVENT_B acetonitrile CH:FLOW_GRADIENT 0.01 min 80% B, 20 min 20% B, 20.5 min 80% B, 34 min 80% B. CH:FLOW_RATE 0.15ml/min CH:COLUMN_TEMPERATURE 45 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME ABI Sciex 6500+ MS:INSTRUMENT_TYPE QTRAP MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS SCIEX OS #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak intensity MS_METABOLITE_DATA_START Samples met6KO_0h_1 met6KO_0h_2 met6KO_0h_3 met6KO_2h_1 met6KO_2h_2 met6KO_2h_3 met6KO_4h_1 met6KO_4h_2 met6KO_4h_3 met6KO_6h_1 met6KO_6h_2 met6KO_6h_3 met6KO_15h_1 met6KO_15h_2 met6KO_15h_3 Factors Sample Type:0h Sample Type:0h Sample Type:0h Sample Type:2h Sample Type:2h Sample Type:2h Sample Type:4h Sample Type:4h Sample Type:4h Sample Type:6h Sample Type:6h Sample Type:6h Sample Type:15h Sample Type:15h Sample Type:15h Glyoxylic acid 25407.24246 24986.5724 17317.10998 26770.15027 21797.06114 20014.87905 24634.97331 31147.3733 24615.82164 23279.00171 16822.29345 18859.21448 29241.84106 26747.93717 26126.77602 Glycolate 7649.014012 7353.474631 5616.51433 11822.75516 11768.24683 3736.182774 7118.487372 3464.911923 12210.49851 3969.286731 4994.391928 3931.797558 3741.671386 5360.127403 13910.49453 Pyruvic acid 18510.85828 33371.09977 18735.69765 17521.03929 18597.4599 34362.03831 17858.1259 30576.50026 29415.52977 21031.07754 21942.21483 28208.51694 16584.14025 22512.50521 34062.1068 L-Lactic acid 1525277.893 1790656.379 1271707.073 1481280.985 1761864.205 1569890.373 1578692.193 2211492.04 1983169.043 1949017.334 1957885.782 1417903.912 2305032.318 3350484.92 2110753.867 acetoacetate 383186.6881 883016.3394 591948.7823 680155.3747 614275.7969 423780.8788 1076996.856 1120993.43 641756.0697 720359.1134 830760.9394 661957.5573 469072.3516 760920.5121 484769.3909 Glyceric acid 37720.4768 49343.21795 52298.75798 47412.61953 48273.40104 58521.27153 98358.57273 144538.0472 193360.8897 82597.1634 131678.8292 42796.03499 144212.3684 112718.5304 110493.6343 Uracil 605595.8838 1549214.305 1616035.117 359981.5369 319041.7609 766988.7958 8164275.928 9772534.73 10193470.39 14017897.21 17104037.21 1395124.723 12664483.85 11679895.61 11824530.65 Fumaric acid 171295.6984 249425.0934 443902.0781 230523.0183 126486.5449 376124.1702 1033258.165 1066301.716 1192424.261 1284025.993 1245064.052 267143.502 1149765.289 1187581.279 1083684.057 Maleic acid 564521.5212 1050112.046 761024.0268 537110.0088 603594.7886 894437.5218 611335.8368 648373.023 483074.951 435631.8581 824990.5549 756806.6678 1093312.51 835494.7793 759465.2412 Alpha-ketoisovaleric acid 171184.1479 288018.45 323254.3014 199759.9949 142685.1984 335986.3978 935391.0716 1132635.301 1220650.566 1206078.297 1257105.672 254610.465 1095361.809 1229546.81 1013186.855 Guanidoacetic acid N/A N/A N/A 5578.119887 N/A 21760.01045 N/A 15747.7349 22203.4417 N/A 23921.38161 N/A N/A N/A 30309.84167 Succinic acid 467880.1017 422945.2145 631916.8863 598130.4226 448652.1579 1217356.684 1333286.794 1772211.321 2330247.569 1992729.995 1626099.943 570255.0009 2007958.09 1558016.299 1750664.211 Methylmalonic acid 533707.4577 459394.164 759467.9672 643181.0015 526659.8248 1391016.165 1522861.792 2122571.32 2689022.461 2235721.195 1845884.093 642866.6627 2296794.419 1821622.567 2031621.313 3-Methylthiopropionic acid N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A Nicotinic acid 27591.85085 N/A N/A N/A N/A 115880.6732 N/A 117145.3089 N/A N/A N/A N/A 59877.38057 N/A 89889.06765 Taurine 20885.53429 61239.31908 16876.23789 21213.50068 32515.37283 20466.42817 12467.94849 32637.99179 126670.3208 13474.62793 13085.33316 17233.44748 31597.05222 29850.45703 35864.43568 Pyroglutamic acid 19505.7625 50181.72893 36035.61384 40757.99529 51530.91608 43413.03331 55189.2252 92576.99855 270012.5673 80695.7862 70399.73041 18055.76556 82538.56761 75894.75947 73804.32119 Citraconic acid 626213.5992 446672.4793 694440.2344 575451.3452 554862.3923 909561.0033 588830.4842 668627.5963 701920.0551 416054.4132 459763.2096 547554.7083 705655.782 701595.542 1094684.433 2-Ketohexanoic acid 25439.91808 52078.3476 41841.59706 9772.513152 14261.98794 18082.35321 25445.74345 27090.44524 27353.77666 24854.78049 24378.52058 32176.00037 19165.71129 20103.57375 26923.5902 N-Acetyl-L-alanine 16237.36642 28524.35137 16484.86798 12597.79057 18162.59618 17191.38527 37797.41649 42420.58817 191444.8315 64435.23855 46803.93849 17580.49306 69728.55203 54326.57949 53316.34345 Oxalacetic acid 19756.75338 23560.09273 10643.51818 37536.46475 17479.32064 20316.47588 29083.93518 44880.72924 29020.84406 25309.21873 24462.79516 23780.73114 25880.25965 61709.39825 17866.79018 Hydroxyisocaproic acid 54946.47399 31778.01155 24463.358 42064.71087 24178.96166 42007.35209 27910.07873 44618.80701 43168.81998 27307.56838 44446.98892 21546.29688 36386.82483 69607.60783 67616.02666 L-Malic acid 1347075.899 2510993.042 2976275.198 1826402.782 1662399.093 3798787.347 10219306.13 9897423.188 11687682.59 12014668.53 11477802.84 1803961.259 10744046.64 11841360.98 10761581.14 Hypoxanthine 38395.22106 89329.56732 92017.29327 77694.03262 54434.27559 175861.8665 3101464.801 3419843.731 3033036.243 6212557.449 7291154.95 77110.9148 5075233.958 4108284.719 4831019.74 p-Aminobenzoic acid N/A N/A N/A N/A N/A 93818.34743 54472.07974 76196.14848 104837.0635 84819.48385 N/A N/A 58862.95277 N/A 98622.9289 4-Hydroxybenzoic acid 186364.6532 288352.0576 161047.2882 288198.336 368618.7601 207105.3359 138789.1597 238032.8111 242165.5014 189076.4569 214417.4006 122899.5634 192761.7276 256079.0955 314979.6608 Acetylphosphate 144608.6527 383393.0749 347091.5468 279861.1618 219610.0777 398779.4035 216726.1885 324678.0096 173827.6759 159172.3083 281665.5021 350322.663 276709.2032 341518.6183 358858.773 Carbamoyl phosphate 47377.15208 115610.7955 66751.16265 143714.7262 135366.7724 296274.0087 1840431.452 2040710.471 1955404.044 2063646.723 2020003.174 47737.91682 1950486.39 2799681.54 2229253.545 Oxoglutaric acid 445344.6425 382171.655 159609.345 888040.4618 126080.8624 61782.01249 323516.4258 153329.5643 117891.6853 218897.6253 215931.8592 225409.2903 529415.7179 167851.8039 473211.023 Phenylpropiolic acid 77059.47847 74050.32948 98820.41009 137200.4651 71655.37801 77716.07874 130778.3779 82674.66675 N/A 44283.78223 126022.4008 82353.15426 164671.6316 58277.47088 86440.96987 2-Oxo-4-methylthiobutanoic acid 33281.69334 42451.95435 43547.85165 17987.08892 20755.77427 46531.87058 12753.57519 19626.77647 7935.357838 9527.146815 7104.447201 39105.44549 27924.89651 20956.72073 18816.71338 Citramalic acid 423239.7238 1068314.447 1001570.774 203600.4984 198013.907 321019.3724 242816.384 350831.9013 335965.9117 342537.4459 223964.57 842158.4207 357419.8127 128941.2695 199927.0743 3-Methylphenylacetic acid 36660.07398 55488.13498 177326.506 131165.4552 134797.6436 176593.0564 128233.8296 153450.5051 82405.89474 95073.62807 30855.88484 100294.3028 168693.7035 210717.3837 162728.3101 Xanthine 66824.3008 332879.9239 52003.13 129061.1111 121523.4107 44681.46605 896643.2226 1423602.971 2609554.585 1385741.436 1502910.366 60120.0906 855763.0132 814658.7641 1180456.929 Ortho-Hydroxyphenylacetic acid 100313.5689 67355.11529 114876.7894 62845.65677 72118.02108 127966.5676 51824.74498 117902.412 72740.86662 77214.69145 55400.08323 107647.8332 136926.3613 99127.28288 145001.3784 2,4-Dihydroxybenzoic acid 23765.40431 33318.17877 48845.74301 30737.31864 40838.33428 44220.27843 39943.74805 8969.964605 20220.10683 19921.70593 69231.00229 47199.71093 74133.29467 26399.58391 44026.32007 Orotic acid 1929974.27 4615109.206 4605137.235 802418.7 737993.2038 1582807.595 480888.6599 709856.9877 426243.5475 240790.5986 297970.799 3689404.212 87342.19147 112417.8689 105251.115 4,5-Dihydroorotic acid 173622.5865 182011.9212 242046.8301 208349.9276 125004.1042 218033.683 225915.3944 174369.3003 235683.8681 164536.6332 185960.8267 184888.8913 174854.5585 210160.3089 364574.5437 Allantoin N/A N/A 7284.392583 N/A N/A N/A N/A 3345.549937 N/A 2620.385335 N/A N/A 2946.823002 N/A N/A Aminoadipic acid 156688.9408 507117.7655 525222.4974 39249.08139 25883.10663 63267.77281 46966.95269 54370.72536 49525.45012 30091.89988 38378.48761 446090.0622 18943.79926 23420.65562 13098.42743 Indole-3-carboxylic acid 1734.524155 2506.209242 4407.13108 4269.35935 4006.997405 6449.537223 21572.15453 27200.61394 40212.50045 51233.60073 33433.69313 4949.865802 19464.80859 32291.25589 32109.88597 Phenylpyruvic acid 48486.05665 43218.27846 35095.42184 30748.63867 32130.11859 88511.52232 55433.52255 65801.37091 45941.58158 38326.72666 26769.57796 55355.35534 52368.10591 44937.38237 63235.19087 Vanillylmandelic acid 8227.673509 9226.049424 9922.284664 12415.16882 14010.17629 9897.606178 9091.051921 15427.96487 12538.67514 13467.91496 12009.99459 9383.844859 4506.038434 7179.506717 8872.338093 Phenyllactic acid 4435.47835 9079.181837 5423.27169 4806.859978 7720.454313 19599.1153 39742.84753 40269.79753 81864.83267 56108.70834 58752.9159 4067.698619 44767.2878 56600.73573 60800.06516 Quinolinic acid 89641.45847 149644.6108 187068.4198 153811.0613 150209.8708 257811.1829 425391.6518 480532.7721 515826.1846 465317.8903 486137.5042 123329.0754 413838.928 295110.1289 390516.5831 Phosphoenolpyruvic acid 85257.74311 104731.133 85516.00016 116668.4752 89573.83236 117823.0835 184485.6276 232354.7714 176111.2025 146968.6766 215896.391 70661.32466 126624.0152 75184.88468 68680.29362 Uric acid 228223.4991 164715.0761 268862.6552 149825.141 174375.5203 153318.4127 570075.7015 480709.2588 232879.2297 91907.90793 92818.27688 297031.987 183512.1595 1423523.228 377020.5074 Dihydroxyacetone phosphate 854372.8461 1076818.029 700175.8067 417525.0232 362675.0811 548436.3597 447941.6475 464350.754 383664.6213 366540.8078 216306.4246 708360.7046 217879.2069 140086.4792 211672.2415 D-Glyceraldehyde 3-phosphate 1171636.834 1356992.419 965457.7438 590103.013 484716.5703 754497.1463 550790.9119 560614.3645 487103.994 459571.3485 308266.2352 902559.916 316478.627 173114.8845 246476.0122 Glycerol 3-phosphate 180611.3435 301650.9475 118610.3808 70800.17571 57479.16023 45557.90441 58446.01447 78962.26228 410705.8304 76555.71778 68725.0426 101904.1777 65629.56665 48400.51246 62575.51821 Shikimic acid 1720.328566 8552.208364 3101.148611 N/A N/A N/A N/A 3466.418885 2153.024526 3868.362561 N/A 3937.943662 2863.068897 N/A N/A cis-Aconitic acid 116537.995 239677.3469 297954.4476 134087.389 76997.19248 344325.4719 281541.8189 360274.0825 567598.8051 706898.2403 519795.0248 159825.777 2673652.998 3167117.81 3824006.232 Allantoic acid 37069.59912 99341.50911 103823.1505 48331.70875 59092.45281 101192.604 97778.26419 126517.1902 97973.55806 67930.14796 78983.73657 97784.71391 46994.21661 36142.33428 41305.73629 Ascorbic acid 33586.78558 61431.69147 34361.1101 39042.34733 43529.04462 49635.94392 30021.27123 68871.2968 88260.56286 51685.11814 34926.39869 36513.55395 85388.47663 52023.89442 82918.72848 2-Isopropylmalic acid 3319908.219 10004742.46 10752748.86 981005.8918 785959.8977 1900253.484 1399921.705 2038503.494 2560697.355 1421878.534 1570263.372 7949461.444 858226.85 622368.3965 836054.6836 Ureidosuccinic acid 39376.73178 79589.34722 72620.5689 13880.12382 14381.65731 25969.75131 7283.245161 12046.81025 7600.048398 7311.090669 5236.244467 54910.1039 927.4319532 2423.14853 3610.26664 Pyrophosphate 82979.84845 162721.5113 137767.9677 46418.57456 40339.01024 68280.17427 96090.3592 109946.3377 83578.73523 103700.2612 73093.94224 97927.46165 112745.7539 76683.98 66969.30269 Gluconolactone 8186.312993 1523.40547 2347.366408 2046.759453 3665.762867 8866.055699 2564.008808 4684.760394 9341.947544 N/A 13608.62608 4089.750901 3185.746324 2736.997113 2741.7847 myo-Inositol 99423917.67 87129307.51 85657654.19 89568829 87431231.49 93287368.58 96987947.69 104071076 85506872.62 85878264.81 87386115.11 80714931.32 95825163.69 103750010.1 91077718.67 4-Hydroxyphenylpyruvic acid 4646.698714 2526.991382 N/A N/A 3879.681905 4679.87623 6033.25631 N/A 6098.138672 4479.563056 2780.364201 5774.517597 N/A 6342.723511 3729.886662 L-Homocysteic acid 985.769862 10775.2917 5580.02034 13103.87718 20613.5238 36673.47861 75781.55086 96067.28645 96125.59139 41308.90233 75999.92591 5419.542354 33231.33355 42908.24796 29122.89646 4-Pyridoxic acid 219178.0689 196864.7625 1270298.276 193358.4838 1052977.549 241227.2383 273248.9211 519053.8023 290146.2994 424963.5103 608482.24 215377.881 484031.489 232537.217 329942.539 3-Phosphoglyceric acid 21541.95963 6605.936036 5406.678362 5395.062742 25294.21669 10764.88621 5594.424012 6615.137993 5587.512888 4097.977993 2202.179203 3319.306782 10125.22469 7223.272823 8031.009759 Indoleacrylic acid 79183.24988 64014.01696 76159.71175 163205.1726 119486.1291 368997.1307 149064.4357 38522.75706 38789.52554 42505.02549 69963.3404 47232.7403 78230.70868 94749.64427 150132.1127 Kynurenic acid 460907.3833 1027565.943 1154159.211 407072.4599 337133.0027 608216.5394 1377735.705 1186774.861 844724.256 1406139.358 1146073.509 1089428.028 853256.9322 898465.0858 1053752.344 NA 207594.8676 232258.8384 396666.2617 34561.68207 35985.64921 164845.7372 56102.41392 207665.1836 630950.5658 434790.9565 346294.9517 286998.9862 169768.059 601416.8692 723134.7543 Isocitric acid 93614.79712 25571.06544 13702.66248 21671.63643 11223.36082 27431.42896 20213.8496 31614.46659 9721.077083 19743.69219 7059.834275 34498.87848 23184.54892 8866.362324 N/A Citric acid 71601.68188 533680.7209 370281.1449 21981.35867 209666.0201 892830.9509 N/A 1881168.372 232922.0786 N/A N/A 99556.63434 N/A N/A 46868.69841 2-Keto-L-gluconate 9034.673313 17441.04368 24882.84279 4949.456449 10048.03523 8185.819497 6120.637167 21323.25494 17604.55318 9785.837448 16751.32192 29221.90548 8638.887863 7333.231173 8938.168483 Gluconic acid 499666.9101 621984.094 995556.4577 265298.7171 300580.0529 342862.0343 240651.0118 554384.36 552843.8262 200400.601 246421.2243 851008.8661 131145.8522 167530.9928 254906.619 D-Erythrose 4-phosphate 315175.6835 613391.098 544637.144 377880.2618 352754.7194 658900.1149 425187.3022 526897.326 315129.6852 287355.2564 442955.1974 468715.7384 482949.4801 499204.3062 492172.0789 Xanthurenic acid 39424.12855 56950.13289 17209.14783 28802.64025 10948.76812 31875.60271 16755.98089 N/A 15833.36836 13260.34738 14179.12786 N/A N/A N/A 18725.84132 (R)-lipoic acid N/A N/A N/A N/A N/A N/A N/A N/A 2831.446324 N/A N/A N/A N/A N/A N/A Glucaric acid 5124.285029 6413.202732 9116.817865 6011.447718 3218.886928 6809.4495 7888.009119 6982.557319 15368.72501 15593.80129 23114.83357 3068.794098 33806.10912 38878.5115 31777.88901 Deoxyribose 1-phosphate 37393.30334 82286.22871 121990.7023 36970.8137 31797.25338 106814.8525 146672.2292 278124.6798 206581.9513 231048.1674 187222.5962 85224.62207 185306.4377 163558.195 172719.5514 Pantothenic acid 66655.94055 113843.9464 109899.1003 186875.4664 181303.2398 355661.3443 294309.3959 364079.9696 724037.7413 236269.1574 276148.5865 121541.1492 137736.5452 138543.041 159410.5734 Prephenate N/A 2739.738965 2504.880628 N/A 2107.989259 N/A 2431.227316 N/A N/A N/A N/A N/A N/A 3597.705068 2622.132476 Deoxyuridine 40982.10174 42514.60721 35849.95991 34489.07627 36480.29825 50230.76186 37020.25712 37146.24937 32729.763 26300.45126 42359.87881 44101.30652 53054.10987 43581.60358 41758.02882 Ribose 1-phosphate 325190.4095 575594.8706 540110.6358 291004.31 260291.7046 436701.2697 618614.7159 762865.7059 723505.8541 750978.568 712685.9409 508119.4709 528987.4921 442743.3458 401908.9524 Thymidine 9534.072832 6240.267307 16319.2662 4600.412112 8461.89012 9345.273588 8907.372333 15970.5223 9420.958335 8511.771999 9492.43356 3842.450507 11734.69765 14384.73617 14920.81409 Uridine 1431.964475 2604.032356 5827.278113 3841.973797 10006.90974 15563.76048 209266.4938 263599.8241 282183.2042 399025.5625 488888.6803 3211.240387 355338.4909 323222.6262 335504.1703 Deoxyinosine N/A N/A N/A 3281.625488 N/A N/A N/A 4595.248991 N/A N/A N/A N/A N/A N/A N/A Shikimate 3-phosphate 321637.8865 327620.1828 470437.1449 20075.05907 30273.25313 86846.82029 13314.76177 154136.4872 458512.1969 312037.1198 219233.982 219954.2384 149040.4987 394304.0316 416461.6724 6-Phosphonoglucono-D-lactone 7171.900069 14853.942 17595.06002 4193.601366 12837.04606 22182.8235 16284.96352 26697.66966 18567.81762 13979.66636 13942.47628 17442.20467 9617.951331 12101.97586 30570.43918 hexose-phosphate 3957920.532 7336488.294 6649425.776 4583205.095 4220776.869 6766418.632 4703586.961 6524379.896 4328419.833 3676912.046 4954433.057 5778806.821 5466351.572 5557960.433 5886869.583 Glucose 1-phosphate 32712.58953 100247.0912 76445.20738 62136.89254 45474.30233 99517.70131 91104.64049 114738.9251 93493.54734 86628.2644 99226.35234 105882.3417 106984.2353 111866.3859 91608.23191 Glucose 6-phosphate 494424.7874 915666.9141 889305.6247 539887.797 541644.6534 925822.9739 591274.0408 818823.547 534880.0008 493809.1186 628012.5476 743544.0027 726931.9094 745857.7847 822779.3514 Fructose 6-phosphate 501083.402 1017856.601 983080.7198 586241.7412 511286.088 995331.9274 559347.694 744713.2454 517556.1768 468390.7717 609346.4943 778810.5018 727897.7016 755221.0706 766466.4168 Glyceric acid 1,3-biphosphate 2830582.089 1680851.696 4404352.599 1873658.36 2436143.773 5151850.12 2775523.531 3428077.672 1769170.942 2324156.631 1416422.942 4119324.748 3854214.075 3735443.643 3887761.534 2,3-Diphosphoglyceric acid 17505.43897 12123.36701 5233.674946 11991.63101 14926.23901 14378.03175 20140.35333 21283.90812 22705.10078 16170.79928 7039.881289 4341.975731 14405.90611 31150.86212 24870.75886 S-Ribosyl-L-homocysteine 106455.6427 79844.37904 77054.11888 62068.86194 40267.5871 57659.55667 77337.43869 108371.7598 1345098.479 90952.90886 72666.42249 79513.86954 41211.2768 53606.12663 115262.3212 Inosine 160878.3302 443449.235 364593.6588 306173.537 291192.1128 701041.9418 12424705.58 15331938.1 16337305.7 23351025.5 28042368.9 337431.0993 19871261.73 17652546.55 20665874.18 6-Phosphogluconic acid 77837.2833 102223.8466 145155.846 24262.22994 53201.17324 54380.32 50021.80288 30921.77172 163942.4848 47427.00247 51560.2123 70014.00127 15054.95409 34911.03741 22521.30117 Xanthosine 48985.59447 110025.0046 60375.74971 32163.51802 24380.53096 51088.11261 835160.663 905919.9181 1205721.898 878734.6852 1116076.072 61896.36878 433338.8352 395462.2783 403519.6184 Sedoheptulose 1,7-bisphosphate 543542.7802 978382.9149 902426.028 605829.7142 497729.0668 896472.4482 738162.9284 1077020.07 416745.6936 420260.7244 744651.6446 767950.5378 1032905.386 1011462.2 765615.241 N-Acetyl-glucosamine 1-phosphate 126211.4576 207850.9666 172988.037 105681.287 85414.02913 141749.3437 167800.0978 185992.0987 220429.7568 208903.7482 173642.9138 168822.1017 262989.4786 200233.9224 247624.7427 Glutathione 5113667.03 11880329.55 8188631.172 2257580.05 2097896.263 4548708.198 6464659.527 7644724.005 6467557.161 8729260.556 4329992.057 13818593.02 4981130.758 2694836.346 4794578.417 dUMP 9655.531229 6824.383343 8757.050557 6031.317627 4345.162834 7803.414459 7914.063324 3693.664081 4842.453411 3792.349727 7674.696007 9521.038138 5751.184543 4519.082609 3232.818488 Geranyl-PP N/A N/A 5568.514745 3644.561783 6250.948108 4628.781735 5117.959258 3567.678669 N/A N/A 2999.620645 3572.688855 3962.947818 3513.953569 4262.720404 Octulose?8-Phosphate 103043.5366 170568.8706 139973.0336 70652.40482 88828.45114 123186.6944 108177.8416 144133.047 107054.44 79362.16648 110230.3249 141321.9711 69201.01734 79112.01443 74444.98463 5-Thymidylic acid 14713.6331 16758.65628 10427.54215 12614.81887 16152.22314 20963.52902 20011.50415 14029.11841 45935.85949 14791.12669 14987.97556 8684.894102 9634.270591 7523.095129 17471.98955 Cyclic AMP 57009.22142 74650.16408 63221.89067 111893.1641 106430.7855 72749.74133 200065.0755 221906.9784 3003869.407 223249.7229 149624.9016 70010.89917 94254.95015 110078.6539 233781.6758 Fructose 1,6-bisphosphate 20556.76682 2835.618006 11842.7945 5337.986759 10250.99626 6472.630647 8889.759519 18183.16736 13726.19635 4418.244411 19459.33139 10294.78778 13907.37759 22194.03573 12902.86077 Sucrose 1013897.511 1868892.335 1745346.5 7618343.966 6614294.088 14649081.6 87645492.2 103148590.2 112543568.1 132586781.2 115474466.7 1454030.332 218335048.2 191260042.6 209195008.2 Trehalose 978608.3531 1821784.735 1986513.831 8380397.035 6820571.768 15636539.34 88050721.6 102113208.7 113196920 141290130 133378613.2 1608310.919 217711612.2 188312548.8 193283757.3 Cellobiose 6609.869772 2708.928791 2530.5311 3702.54428 10331.2394 4184.223209 15182.77249 18182.63949 23019.43202 21900.12767 22462.60112 6781.060331 40485.81842 28003.35473 32583.12098 Orotidylic acid 50906.03936 77182.32544 97758.12461 165175.8196 94031.67668 146438.6951 101348.6328 65689.88423 52033.26002 52202.62123 135487.2732 180174.9882 146291.4743 150861.4135 59454.67653 Farnesyl-PP 12576.69132 13396.93399 5569.664202 4358.063393 1852.104585 8032.611659 2597.169521 11229.97312 5824.439505 4220.741236 7622.536874 7921.620546 11250.86673 12124.99461 14962.77822 S-Adenosylhomocysteine 1106741.63 2337504.818 1976350.806 694031.8309 504800.4929 916812.5348 1654038.602 1884906.951 1954829.615 1946518.809 1666299.724 1726308.199 1353346.982 1015993.457 1231008.913 dCDP 9437.242546 15105.35674 14813.60181 14293.26886 13746.78541 16183.78625 11023.4604 19911.2708 20460.19839 15104.60273 14006.8106 10763.27019 14554.581 11061.5735 16123.14524 Phosphoribosyl pyrophosphate N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A Deoxycholic acid 3253.327413 2972.836695 4348.935282 5099.301634 10406.8093 6067.823159 4984.798516 7260.187697 N/A 2943.159524 4465.615563 6359.728688 4055.042661 5961.494151 6482.139794 octulose-1_8-bisphosphate 74232.27323 57981.76487 129703.0807 62679.8329 86399.16907 146961.3017 65970.69808 129721.1198 59858.3854 70545.00647 62331.23972 117923.388 104606.571 112322.3118 114167.9412 dTDP N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A CDP 9548.217932 4280.37451 2263.516879 5406.648483 6145.322937 9091.612519 4529.767035 12301.09194 22422.98918 7024.737366 6941.328975 2774.922918 9270.105647 18211.42324 27249.36743 Uridine 5'-diphosphate N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A Cholic acid 5845.793094 3834.116092 2346.762219 6478.084711 6302.109411 4325.425449 9241.969057 9969.651439 6196.976152 6771.158464 4834.70003 10896.04675 N/A 8196.338235 4770.945106 Trehalose 6-phosphate 90789.57182 116715.5216 87029.67203 352746.267 335926.6189 415120.3329 340260.3489 372718.024 195455.2798 172902.5934 241825.0462 83794.5162 200656.3573 220593.1197 184229.5302 Thiamine pyrophosphate 5171.556422 N/A N/A N/A N/A N/A N/A N/A N/A N/A 91039.9363 N/A N/A 120040.1335 N/A Adenosine phosphosulfate 5601.635227 6193.847175 3275.874064 3010.129187 3190.787982 7232.763821 3536.405256 1640.907505 3843.508631 4652.809619 N/A 3555.491885 4371.797901 2691.764482 7838.052592 ADP 1309557.335 1676961.851 1395972.743 1621551.353 1624681.822 2088464.301 2149132.434 2971515.295 2747298.439 2349244.736 2197418.46 1268981.814 2055843.11 2150849.629 1841046.24 dGDP 945968.2245 1255522.853 1009360.308 1197387.124 1144861.429 1495871.926 1634729.5 2407923.72 2042785.238 1777350.113 1745205.457 929216.045 1540880.835 1749850.251 1476596.813 IDP 202832.3383 274713.5689 223496.9987 253822.9742 248576.367 348104.4804 386282.6629 519141.9389 427807.7444 384454.9719 369519.0349 225073.3216 332722.5309 334223.3928 343666.8763 Guanosine diphosphate N/A 1875.194366 N/A N/A N/A N/A N/A N/A N/A N/A 4159.033764 N/A 2573.345048 N/A N/A CDP-Ethanolamine 867.0993059 5546.799714 3795.653988 4046.583656 3744.363921 3749.378663 7572.097523 11324.06801 8465.423974 10241.89716 15926.68098 2064.413848 15337.90803 12680.66531 15607.07613 Flavin Mononucleotide 42165.91245 45924.83924 45656.10589 38726.98496 34505.87218 37451.23584 38972.98185 43991.06264 45018.33506 39173.59503 35375.74021 31711.96746 20937.35526 28836.88935 23844.45454 Cholesterol sulfate 283965.4239 287287.0145 318422.6039 286686.9794 441668.0381 265879.3631 240733.2213 338558.8927 316278.9925 847549.7358 321223.5068 276183.3723 525413.5699 309672.5172 497555.1925 dCTP N/A N/A N/A 2966.200125 N/A N/A N/A N/A N/A N/A 4313.332514 N/A 3754.230595 N/A 2134.416163 Deoxyuridine triphosphate N/A N/A N/A N/A N/A 2121.501129 N/A N/A N/A N/A N/A N/A N/A N/A N/A Thymidine 5'-triphosphate N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A Cytidine triphosphate N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A Uridine triphosphate 59208.57643 77515.00793 120413.0743 181445.5958 147301.2323 374091.6637 191050.967 328657.3469 127019.3266 253324.8138 186518.6249 44496.4633 195083.4996 125407.4103 337245.3144 Citicoline 14837.13594 11917.64714 15360.60647 9127.742567 15035.37065 12915.59121 22087.38307 31225.52659 32367.7629 24003.18994 28649.15549 11612.5115 22976.66676 13165.83968 17970.42511 Deoxyadenosine triphosphate N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A Taurodeoxycholic acid N/A 2267.746793 2958.964171 3036.314874 N/A 5665.971225 N/A 11869.08674 3245.931308 7012.812676 N/A 3289.274102 3617.792533 4298.500967 4045.874768 Adenosine triphosphate 173708.6786 468140.4877 209129.7465 1165262.531 1077710.61 1292093.77 370991.0643 378882.8784 1350333.156 513311.1037 107225.9849 286267.8267 533905.9539 726643.1207 818478.8072 dGTP 357974.736 379222.4113 179401.1361 1246957.718 1590460.785 89794.11324 258170.3111 386328.6976 1742365.497 715902.6246 643453.4188 78827.82495 309900.9631 225409.782 620714.9909 Guanosine triphosphate N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A 2245.799177 Uridine diphosphate glucose 1639795.807 1863343.827 1616736.919 2093493.112 1921516.049 2583596.84 4377498.25 4924261.051 4326839.468 4323176.878 4687407.806 1420923.211 5990381.44 5495212.381 5271309.902 Uridine diphosphate glucuronic acid N/A N/A N/A N/A 2695.968241 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A ADP-glucose N/A N/A N/A N/A N/A N/A N/A N/A 3384.594413 N/A N/A N/A N/A N/A N/A Guanosine 3',5'-bis(diphosphate) 2953.380494 N/A 3819.435459 2319.511212 N/A 2307.633417 N/A 2916.597862 N/A N/A N/A N/A 2841.608013 3959.014176 N/A Uridine diphosphate-N-acetylglucosamine 916155.2114 1107297.079 1097343.991 952845.507 844256.1009 1226615.868 1042834.509 1046480.991 1058763.388 1005801.224 916524.7538 828646.1882 1220624.758 1061520.931 1072753.784 Oxidized glutathione 4477292.041 7764270.462 10552251.11 4139689.549 3430606.877 6771474.725 7051621.666 8514999.982 11662613.23 10002374.18 10844325.55 6410264.037 5314732.147 7924905.317 6799593.735 NAD 13797402.59 14101429.46 14197420 16454278.83 14410813.02 11787624.38 19465994.86 21896771.69 16561092.9 17080611.66 20552737.52 12349301.26 18007939.87 19763907.91 16523587.01 NADH 798325.307 1033663.362 902260.6153 569441.8731 583599.9039 1002874.722 1093006.191 860162.4944 1275260.584 1253087.037 1164195.136 1125543.039 466862.9862 481957.0255 576331.2446 Dephospho-CoA N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A Cyclic-Di-GMP N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A NADP 822825.8664 998699.2902 846070.479 1287491.541 1159342.045 1246876.174 1139300.035 1561607.415 1160112.679 1003828.119 772361.3884 668969.7792 944012.9744 1208459.821 938202.8951 NADPH 11765.71853 26186.30476 28357.36405 40745.36911 26624.62733 73155.50086 30663.84186 47052.12525 76630.93968 55025.7353 36567.05233 19732.57334 19399.13627 25351.80114 17783.22882 Coenzyme A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A acetyl-CoA N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A Propionyl-CoA N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A Butyryl-CoA N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A Acetoacetyl-CoA N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A Malonyl-CoA N/A N/A N/A 3642.124235 N/A N/A 2135.100097 N/A N/A N/A N/A N/A N/A N/A N/A 3-Hydroxybutyryl-CoA N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A succinyl-CoA 2338.079831 N/A 4936.408732 2252.222392 N/A N/A N/A 3563.319829 4270.932607 N/A 2486.234564 2204.355163 N/A 5786.98459 4350.731388 3-Hydroxy-3-methylglutaryl-CoA N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A 2-deoxyglucose-6-phosphate N/A 2192.182451 N/A 1926.759455 N/A N/A N/A N/A 1896.491417 N/A 4286.763687 2704.219185 2506.608888 N/A N/A D-2-Hydroxyglutarate 809250.8443 1677127.336 1560537.069 321985.1706 285911.713 489335.7071 736079.2449 805412.8451 869992.6567 745234.3831 527490.6367 1273013.12 706090.7368 376554.7901 457807.052 13C-Serine N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A 13C-Methionine N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A 13C-taurine 6112.117711 8292.282112 3509.141268 4838.212471 3368.683232 8951.648108 3949.495648 6229.677361 4089.401171 6018.511161 4516.362417 3262.371225 N/A 2923.002729 7154.972893 13C-Xanthine N/A N/A N/A N/A N/A N/A 2412.675341 N/A N/A N/A N/A 2543.000622 N/A 3284.943158 N/A MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Glyoxylic acid Glycolate Pyruvic acid L-Lactic acid acetoacetate Glyceric acid Uracil Fumaric acid Maleic acid Alpha-ketoisovaleric acid Guanidoacetic acid Succinic acid Methylmalonic acid 3-Methylthiopropionic acid Nicotinic acid Taurine Pyroglutamic acid Citraconic acid 2-Ketohexanoic acid N-Acetyl-L-alanine Oxalacetic acid Hydroxyisocaproic acid L-Malic acid Hypoxanthine p-Aminobenzoic acid 4-Hydroxybenzoic acid Acetylphosphate Carbamoyl phosphate Oxoglutaric acid Phenylpropiolic acid 2-Oxo-4-methylthiobutanoic acid Citramalic acid 3-Methylphenylacetic acid Xanthine Ortho-Hydroxyphenylacetic acid 2,4-Dihydroxybenzoic acid Orotic acid 4,5-Dihydroorotic acid Allantoin Aminoadipic acid Indole-3-carboxylic acid Phenylpyruvic acid Vanillylmandelic acid Phenyllactic acid Quinolinic acid Phosphoenolpyruvic acid Uric acid Dihydroxyacetone phosphate D-Glyceraldehyde 3-phosphate Glycerol 3-phosphate Shikimic acid cis-Aconitic acid Allantoic acid Ascorbic acid 2-Isopropylmalic acid Ureidosuccinic acid Pyrophosphate Gluconolactone myo-Inositol 4-Hydroxyphenylpyruvic acid L-Homocysteic acid 4-Pyridoxic acid 3-Phosphoglyceric acid Indoleacrylic acid Kynurenic acid NA Isocitric acid Citric acid 2-Keto-L-gluconate Gluconic acid D-Erythrose 4-phosphate Xanthurenic acid (R)-lipoic acid Glucaric acid Deoxyribose 1-phosphate Pantothenic acid Prephenate Deoxyuridine Ribose 1-phosphate Thymidine Uridine Deoxyinosine Shikimate 3-phosphate 6-Phosphonoglucono-D-lactone hexose-phosphate Glucose 1-phosphate Glucose 6-phosphate Fructose 6-phosphate Glyceric acid 1,3-biphosphate 2,3-Diphosphoglyceric acid S-Ribosyl-L-homocysteine Inosine 6-Phosphogluconic acid Xanthosine Sedoheptulose 1,7-bisphosphate N-Acetyl-glucosamine 1-phosphate Glutathione dUMP Geranyl-PP Octulose?8-Phosphate 5-Thymidylic acid Cyclic AMP Fructose 1,6-bisphosphate Sucrose Trehalose Cellobiose Orotidylic acid Farnesyl-PP S-Adenosylhomocysteine dCDP Phosphoribosyl pyrophosphate Deoxycholic acid octulose-1_8-bisphosphate dTDP CDP Uridine 5'-diphosphate Cholic acid Trehalose 6-phosphate Thiamine pyrophosphate Adenosine phosphosulfate ADP dGDP IDP Guanosine diphosphate CDP-Ethanolamine Flavin Mononucleotide Cholesterol sulfate dCTP Deoxyuridine triphosphate Thymidine 5'-triphosphate Cytidine triphosphate Uridine triphosphate Citicoline Deoxyadenosine triphosphate Taurodeoxycholic acid Adenosine triphosphate dGTP Guanosine triphosphate Uridine diphosphate glucose Uridine diphosphate glucuronic acid ADP-glucose Guanosine 3',5'-bis(diphosphate) Uridine diphosphate-N-acetylglucosamine Oxidized glutathione NAD NADH Dephospho-CoA Cyclic-Di-GMP NADP NADPH Coenzyme A acetyl-CoA Propionyl-CoA Butyryl-CoA Acetoacetyl-CoA Malonyl-CoA 3-Hydroxybutyryl-CoA succinyl-CoA 3-Hydroxy-3-methylglutaryl-CoA 2-deoxyglucose-6-phosphate D-2-Hydroxyglutarate 13C-Serine 13C-Methionine 13C-taurine 13C-Xanthine METABOLITES_END #END