{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002843","ANALYSIS_ID":"AN004653","VERSION":"1","CREATED_ON":"August 25, 2023, 12:35 pm"},

"PROJECT":{"PROJECT_TITLE":"Leukemia inhibitory factor suppresses hepatic de novo lipogenesis and induces cachexia","PROJECT_SUMMARY":"We performed metabolomic analysis to measure polar and lipid metabolites in the serum from TgLC and TgL mice with Tamoxifen (TAM) injection. We compared the levels of lipid metabolites in the liver from TgLC and TgL mice with TAM injection. The lipid metabolites levels in the liver from Balb/c mice bearing C26 or C26-LIF KO tumors were also measured. Mice under both fed and fasted conditions were included. To investigate whether fenofibrate can restore hepatic lipid homeostasis, TgLC mice were fed with a regular chow or fenofibrate diet. Lipid metabolites in liver tissues were analyzed after TAM injection. A similar effect of fenofibrate on lipid metabolites was observed in C26 tumor-bearing mice.","INSTITUTE":"Rutgers University","DEPARTMENT":"Department of Radiation Oncology","LAST_NAME":"Hu","FIRST_NAME":"Wenwei","ADDRESS":"195 Little Albany St, New Brunswick, NJ 08901","EMAIL":"wh221@cinj.rutgers.edu","PHONE":"7322356169"},

"STUDY":{"STUDY_TITLE":"Leukemia inhibitory factor suppresses hepatic de novo lipogenesis and induces cachexia (Tamoxifen)","STUDY_SUMMARY":"We compared the levels of lipid metabolites in the liver from TgLC and TgL mice with Tamoxifen injection. The lipid metabolites levels in the liver from Balb/c mice bearing C26 or C26-LIF KO tumors were also measured. Mice under both fed and fasted conditions were included.","INSTITUTE":"Rutgers University","LAST_NAME":"Hu","FIRST_NAME":"Wenwei","ADDRESS":"195 Little Albany St, New Brunswick, NJ 08901","EMAIL":"wh221@cinj.rutgers.edu","STUDY_COMMENTS":"Same project as Study:ST002824","PHONE":"7322356169"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Liver_WT_F_1F",
"Factors":{"Genotype":"TgL","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_F_1F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_WT_F_2F",
"Factors":{"Genotype":"TgL","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_F_2F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_WT_F_5F",
"Factors":{"Genotype":"TgL","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_F_5F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_WT_F_6F",
"Factors":{"Genotype":"TgL","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_F_6F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_WT_F_3M",
"Factors":{"Genotype":"TgL","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_F_3M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_WT_F_4M",
"Factors":{"Genotype":"TgL","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_F_4M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_WT_F_5M",
"Factors":{"Genotype":"TgL","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_F_5M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_WT_F_6M",
"Factors":{"Genotype":"TgL","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_F_6M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_F_1F",
"Factors":{"Genotype":"TgLC","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_F_1F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_F_2F",
"Factors":{"Genotype":"TgLC","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_F_2F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_F_3F",
"Factors":{"Genotype":"TgLC","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_F_3F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_F_4F",
"Factors":{"Genotype":"TgLC","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_F_4F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_F_5F",
"Factors":{"Genotype":"TgLC","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_F_5F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_F_6F",
"Factors":{"Genotype":"TgLC","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_F_6F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_F_3M",
"Factors":{"Genotype":"TgLC","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_F_3M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_F_4M",
"Factors":{"Genotype":"TgLC","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_F_4M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_WT_S_1F",
"Factors":{"Genotype":"TgL","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_S_1F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_WT_S_2F",
"Factors":{"Genotype":"TgL","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_S_2F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_WT_S_3F",
"Factors":{"Genotype":"TgL","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_S_3F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_WT_S_4F",
"Factors":{"Genotype":"TgL","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_S_4F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_WT_S_3M",
"Factors":{"Genotype":"TgL","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_S_3M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_WT_S_4M",
"Factors":{"Genotype":"TgL","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_S_4M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_WT_S_5M",
"Factors":{"Genotype":"TgL","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_S_5M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_WT_S_6M",
"Factors":{"Genotype":"TgL","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_WT_S_6M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_S_1F",
"Factors":{"Genotype":"TgLC","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_S_1F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_S_2F",
"Factors":{"Genotype":"TgLC","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_S_2F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_S_3F",
"Factors":{"Genotype":"TgLC","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_S_3F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_S_4F",
"Factors":{"Genotype":"TgLC","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_S_4F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_S_5F",
"Factors":{"Genotype":"TgLC","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_S_5F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_S_6F",
"Factors":{"Genotype":"TgLC","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_S_6F.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_S_5M",
"Factors":{"Genotype":"TgLC","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_S_5M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_Tglif_S_6M",
"Factors":{"Genotype":"TgLC","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"Liver_Tglif_S_6M.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"NC-c26_F_1",
"Factors":{"Genotype":"Con","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"NC-c26_F_1.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"NC-c26_F_2",
"Factors":{"Genotype":"Con","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"NC-c26_F_2.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"NC-c26_F_3",
"Factors":{"Genotype":"Con","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"NC-c26_F_3.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"NC-c26_F_4",
"Factors":{"Genotype":"Con","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"NC-c26_F_4.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"NC-c26_F_5",
"Factors":{"Genotype":"Con","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"NC-c26_F_5.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"NC-c26_F_6",
"Factors":{"Genotype":"Con","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"NC-c26_F_6.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_F_1",
"Factors":{"Genotype":"C26","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26_F_1.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_F_2",
"Factors":{"Genotype":"C26","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26_F_2.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_F_3",
"Factors":{"Genotype":"C26","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26_F_3.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_F_4",
"Factors":{"Genotype":"C26","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26_F_4.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_F_5",
"Factors":{"Genotype":"C26","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26_F_5.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_F_6",
"Factors":{"Genotype":"C26","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26_F_6.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_F_7",
"Factors":{"Genotype":"C26","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26_F_7.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_F_8",
"Factors":{"Genotype":"C26","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26_F_8.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_F_9",
"Factors":{"Genotype":"C26","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26_F_9.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_F_10",
"Factors":{"Genotype":"C26","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26_F_10.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26-lifKO_F_1",
"Factors":{"Genotype":"C26-LIF KO","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26-lifKO_F_1.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26-lifKO_F_2",
"Factors":{"Genotype":"C26-LIF KO","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26-lifKO_F_2.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26-lifKO_F_3",
"Factors":{"Genotype":"C26-LIF KO","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26-lifKO_F_3.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26-lifKO_F_4",
"Factors":{"Genotype":"C26-LIF KO","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26-lifKO_F_4.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26-lifKO_F_5",
"Factors":{"Genotype":"C26-LIF KO","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26-lifKO_F_5.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26-lifKO_F_6",
"Factors":{"Genotype":"C26-LIF KO","Condition":"Fed"},
"Additional sample data":{"RAW_FILE_NAME":"c26-lifKO_F_6.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"NC-c26_S_1",
"Factors":{"Genotype":"Con","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"NC-c26_S_1.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"NC-c26_S_2",
"Factors":{"Genotype":"Con","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"NC-c26_S_2.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"NC-c26_S_3",
"Factors":{"Genotype":"Con","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"NC-c26_S_3.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"NC-c26_S_4",
"Factors":{"Genotype":"Con","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"NC-c26_S_4.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"NC-c26_S_5",
"Factors":{"Genotype":"Con","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"NC-c26_S_5.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"NC-c26_S_6",
"Factors":{"Genotype":"Con","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"NC-c26_S_6.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_S_1",
"Factors":{"Genotype":"C26","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26_S_1.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_S_2",
"Factors":{"Genotype":"C26","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26_S_2.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_S_3",
"Factors":{"Genotype":"C26","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26_S_3.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_S_4",
"Factors":{"Genotype":"C26","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26_S_4.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_S_5",
"Factors":{"Genotype":"C26","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26_S_5.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_S_6",
"Factors":{"Genotype":"C26","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26_S_6.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_S_7",
"Factors":{"Genotype":"C26","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26_S_7.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_S_8",
"Factors":{"Genotype":"C26","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26_S_8.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_S_9",
"Factors":{"Genotype":"C26","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26_S_9.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26_S_10",
"Factors":{"Genotype":"C26","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26_S_10.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26-lifKO_S_1",
"Factors":{"Genotype":"C26-LIF KO","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26-lifKO_S_1.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26-lifKO_S_2",
"Factors":{"Genotype":"C26-LIF KO","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26-lifKO_S_2.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26-lifKO_S_3",
"Factors":{"Genotype":"C26-LIF KO","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26-lifKO_S_3.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26-lifKO_S_4",
"Factors":{"Genotype":"C26-LIF KO","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26-lifKO_S_4.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26-lifKO_S_5",
"Factors":{"Genotype":"C26-LIF KO","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26-lifKO_S_5.mzXML"}
},
{
"Subject ID":"-",
"Sample ID":"c26-lifKO_S_6",
"Factors":{"Genotype":"C26-LIF KO","Condition":"Fasted"},
"Additional sample data":{"RAW_FILE_NAME":"c26-lifKO_S_6.mzXML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"TgLC and TgL mice were injected with TAM. Balb/c mice were injected with C26 or C26-LIF KO cells. Liver was collected and then stored at -80 °C.","SAMPLE_TYPE":"Liver"},

"TREATMENT":{"TREATMENT_SUMMARY":"TgL and TgLC mice were injected with Tamoxifen. Balb/c mice were injected with C26 or C26-LIF KO cells."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"For lipidomic metabolites extraction, 10 mg homogenized tissues were mixed with 120 μL methanol and 400 μL MTBE. After shaking for 6 minutes at 4 °C, 100 μL H2O was added and followed by centrifugation for 2 minutes. Supernatants were dried down for further lipidomics analysis."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Lipidomics method","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Vanquish","COLUMN_NAME":"Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)","SOLVENT_A":"90% water/10% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate","SOLVENT_B":"75% isopropanol/25% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate","FLOW_GRADIENT":"0 min, 25% B; 2 min, 25% B; 5.5 min, 65% B; 12.5 min, 100% B; 19.5 min, 100% B; 20.0 min, 25% B; 30 min, 25% B","FLOW_RATE":"0.2mL/min","COLUMN_TEMPERATURE":"55C"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ACQUISITION_PARAMETERS_FILE":"LCMS_Pos_RT_30min_55C_LongElution"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Plus Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Positive Ionization mode","CAPILLARY_TEMPERATURE":"300","ION_SOURCE_TEMPERATURE":"360","SPRAY_VOLTAGE":"2700","AUTOMATIC_GAIN_CONTROL":"3000000"},

"MS_METABOLITE_DATA":{
"Units":"Ion counts",

"Data":[{"Metabolite":"15:0-18:1(d7) 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}

}