{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001065","ANALYSIS_ID":"AN001743","VERSION":"1","CREATED_ON":"October 3, 2018, 9:40 am"},

"PROJECT":{"PROJECT_TITLE":"Lipidomics analysis for aged mice organs","PROJECT_TYPE":"MS lipidomics analysis","PROJECT_SUMMARY":"Lipidomics studies on samples from aged and young mice.","INSTITUTE":"Takeda Pharmaceutical Company Limited","LAST_NAME":"Ando","FIRST_NAME":"Ayumi","ADDRESS":"26-1, Muraoka-Higashi 2-chome, Fujisawa-shi, Kanagawa, 251-8555, Japan","EMAIL":"ayumi.kawamura@takeda.com","PHONE":"+81-466-32-4216","PUBLICATIONS":"Manuscript is now submitting to Metabolomics"},

"STUDY":{"STUDY_TITLE":"Lipidomics analysis for aged mice brain cortex (part-II)","STUDY_TYPE":"MS lipidomics analysis","STUDY_SUMMARY":"Lipidomics studies on samples from aged and young mice.","INSTITUTE":"Takeda Pharmaceutical Company Limited","LAST_NAME":"Ayumi","FIRST_NAME":"Ando","ADDRESS":"26-1, Muraoka-Higashi 2-chome, Fujisawa-shi, Kanagawa 251-8555, Japan","EMAIL":"ayumi.kawamura@takeda.com","PHONE":"+81466-32-4216"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL/6J mice","AGE_OR_AGE_RANGE":"8 week (“young”) and 112 week (“old”).","WEIGHT_OR_WEIGHT_RANGE":"young: 20.2g (mean), aged: 34.3g (mean)","GENDER":"Male","ANIMAL_ANIMAL_SUPPLIER":"CLEA (Tokyo, Japan)","ANIMAL_LIGHT_CYCLE":"a 12-hour light/12-hour dark schedule","ANIMAL_FEED":"CE-2, CLEA","ANIMAL_WATER":"tap water"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Adipose_51_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Epididymal adipose tissue"}
},
{
"Subject ID":"-",
"Sample ID":"Adipose_53_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Epididymal adipose tissue"}
},
{
"Subject ID":"-",
"Sample ID":"Adipose_55_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Epididymal adipose tissue"}
},
{
"Subject ID":"-",
"Sample ID":"Adipose_57_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Epididymal adipose tissue"}
},
{
"Subject ID":"-",
"Sample ID":"Adipose_59_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Epididymal adipose tissue"}
},
{
"Subject ID":"-",
"Sample ID":"Adipose_52_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Epididymal adipose tissue"}
},
{
"Subject ID":"-",
"Sample ID":"Adipose_54_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Epididymal adipose tissue"}
},
{
"Subject ID":"-",
"Sample ID":"Adipose_56_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Epididymal adipose tissue"}
},
{
"Subject ID":"-",
"Sample ID":"Adipose_58_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Epididymal adipose tissue"}
},
{
"Subject ID":"-",
"Sample ID":"Adipose_60_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Epididymal adipose tissue"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_51_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Liver"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_53_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Liver"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_55_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Liver"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_57_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Liver"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_59_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Liver"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_52_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Liver"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_54_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Liver"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_56_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Liver"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_58_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Liver"}
},
{
"Subject ID":"-",
"Sample ID":"Liver_60_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Liver"}
},
{
"Subject ID":"-",
"Sample ID":"Cortex_51_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Cerebral Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"Cortex_53_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Cerebral Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"Cortex_55_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Cerebral Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"Cortex_57_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Cerebral Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"Cortex_59_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Cerebral Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"Cortex_52_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Cerebral Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"Cortex_54_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Cerebral Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"Cortex_56_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Cerebral Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"Cortex_58_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Cerebral Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"Cortex_60_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Cerebral Cortex"}
},
{
"Subject ID":"-",
"Sample ID":"Femoral_51_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Femoral muscle"}
},
{
"Subject ID":"-",
"Sample ID":"Femoral_53_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Femoral muscle"}
},
{
"Subject ID":"-",
"Sample ID":"Femoral_55_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Femoral muscle"}
},
{
"Subject ID":"-",
"Sample ID":"Femoral_57_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Femoral muscle"}
},
{
"Subject ID":"-",
"Sample ID":"Femoral_59_young",
"Factors":{"Age":"Young 8 weeks","Tissue":"Femoral muscle"}
},
{
"Subject ID":"-",
"Sample ID":"Femoral_52_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Femoral muscle"}
},
{
"Subject ID":"-",
"Sample ID":"Femoral_54_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Femoral muscle"}
},
{
"Subject ID":"-",
"Sample ID":"Femoral_56_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Femoral muscle"}
},
{
"Subject ID":"-",
"Sample ID":"Femoral_58_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Femoral muscle"}
},
{
"Subject ID":"-",
"Sample ID":"Femoral_60_aged",
"Factors":{"Age":"Aged 112 weeks","Tissue":"Femoral muscle"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"All mice were killed by decapitation for sample collection. Collected tissues were immediately frozen by liquid nitrogen then stored -80 C until use. (Femoral, Cortex, Liver, Adipose) tissues.","SAMPLE_TYPE":"Brain","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"Tissues were homogenised in isopropanol (100 mg/mL) for lipidomics."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Tissues were homogenised in isopropanol (100 mg/mL) for lipidomics."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"The liquid chromatography/mass spectrometry system consisted of an Ultimate3000 RSLC system (Thermo Fisher Scientific inc., Sunnyvale, CA, USA) using a gradient elution consisting of mobile phase A (0.01% acetic acid and 1 mM ammonia in MilliQ water) and mobile phase B (0.001% acetic acid and 0.2 mM ammonia in ethanol/isopropanol [3:1])","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Orbitrap XL","COLUMN_NAME":"Waters XBridge C18 (50 x 2.1mm, 3um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Thermo LTQ XL","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"ESI","ION_MODE":"NEGATIVE"},

"MS_METABOLITE_DATA":{
"Units":"peak area",

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