{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001737","ANALYSIS_ID":"AN002828","VERSION":"1","CREATED_ON":"April 3, 2021, 9:21 am"},

"PROJECT":{"PROJECT_TITLE":"1H HRMAS NMR Spectroscopy based Metabolomics of Urinary Bladder Tissues from NMIBC Patients","PROJECT_TYPE":"NMR Based Metabolomics","PROJECT_SUMMARY":"Application of 1H HRMAS NMR Spectroscopy to study malignancy induced metabolomic changes in urinary bladder tissues from 26 NMIBC patients. Predict the possible biomarker of NMIBC in urinary bladder tissues.","INSTITUTE":"Centre of Biomedical Research, Lucknow","DEPARTMENT":"Department of Radiology","LABORATORY":"NMR Based Metabolomics","LAST_NAME":"Roy","FIRST_NAME":"Raja","ADDRESS":"Centre of Biomedical Research, Lucknow, India 226014","EMAIL":"rajaroy28@gmail.com","PHONE":"+919005095427","CONTRIBUTORS":"Anup Paul, Raja Roy, Akshay Anand, Kushagra Gaurav, Mala Sagar, Abhinav A. Sonkar"},

"STUDY":{"STUDY_TITLE":"1H HRMAS NMR Spectroscopy based Metabolomics of Urinary Bladder Tissues from NMIBC Patients","STUDY_TYPE":"NMR Based Metabolomics","STUDY_SUMMARY":"Application of 1H HRMAS NMR Spectroscopy to study malignancy induced metabolomic changes in urinary bladder tissues from 26 NMIBC patients. Predict the possible biomarker of NMIBC in urinary bladder tissues.","INSTITUTE":"Centre of Biomedical Research, Lucknow, India","DEPARTMENT":"Department of Radiology","LABORATORY":"NMR Based Metabolomics","LAST_NAME":"Roy","FIRST_NAME":"Raja","ADDRESS":"Centre of Biomedical Research, Lucknow, India 226014","EMAIL":"rajaroy28@gmail.com","PHONE":"+919005095427","NUM_GROUPS":"2","TOTAL_SUBJECTS":"26 (n=26, malignant, and n=26, benign)"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","AGE_OR_AGE_RANGE":"57.42 ± 4.1 years","GENDER":"Not applicable"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Patient_1_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_2_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_3_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_4_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_5_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_6_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_7_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_8_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_9_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_10_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_11_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_12_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_13_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_14_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_15_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_16_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_17_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_18_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_19_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_20_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_21_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_22_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_23_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_24_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_25_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_26_Malignant",
"Factors":{"Histopathological_Status":"2"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_1_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_2_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_3_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_4_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_5_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_6_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_7_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_8_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_9_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_10_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_11_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_12_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_13_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_14_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_15_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_16_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_17_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_18_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_19_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_20_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_21_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_22_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_23_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_24_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_25_Benign",
"Factors":{"Histopathological_Status":"1"}
},
{
"Subject ID":"-",
"Sample ID":"Patient_26_Benign",
"Factors":{"Histopathological_Status":"1"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Urinary Bladder Tissue (UBT) specimens collected from 26 patients who have undergone surgical treatment for NMIBC at KGMU, Lucknow.Informed consent was obtained from all the patients and protocols were approved by KGMU Ethical Committee. On the basis of visual screening by the surgeons tissue specimens includes NMIBC lesions and adjacent tissues of urinary bladder. The tissues specimens were snap frozen in liquid nitrogen during surgical resection and stored in cryo-vials at -80 degree Centigrade till Spectral recording.","SAMPLE_TYPE":"Tissue Specimen"},

"TREATMENT":{"TREATMENT_SUMMARY":"Stored urinary bladder tissues were thawed to room temperature. Then saline water used to washed these specimens."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"The wet tissues were dissected to weighted of 34 mg then transferred into 50 μl ZrO2 rotor."},

"ANALYSIS":{"ANALYSIS_TYPE":"NMR","LABORATORY_NAME":"Division of SAIF, CSIR-Central Drug Research Institute, Lucknow"},

"NM":{"INSTRUMENT_NAME":"Bruker Fallandden","INSTRUMENT_TYPE":"FT-NMR","NMR_EXPERIMENT_TYPE":"1D-1H","STANDARD_CONCENTRATION":"0.7697 mM","SPECTROMETER_FREQUENCY":"400 MHz","NMR_PROBE":"HRMAS dual probehead","NMR_SOLVENT":"D2O","NMR_TUBE_SIZE":"4mm ZrO2 rotor of 50 μl capacity","PULSE_SEQUENCE":"CPMG, NOESY","WATER_SUPPRESSION":"PR","RECEIVER_GAIN":"2050","TEMPERATURE":"281 ± 0.5 K","NUMBER_OF_SCANS":"128","DUMMY_SCANS":"4","RELAXATION_DELAY":"5.992s","SPECTRAL_WIDTH":"8223.685 Hz","NUM_DATA_POINTS_ACQUIRED":"32K","NMR_RESULTS_FILE":"NMIBC_RR2021_NOESY.xlsx;NMIBC_RR2021_CPMG.xlsx;NMIBC_RR2021_CONCENTRATION.xlsx UNITS:Signal intensity;mM"}

}