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MB Sample ID: SA007406

Local Sample ID:604 Cecum
Subject ID:SU000154
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:6 weeks old
Gender:Males
Species Group:Mammal

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Subject:

Subject ID:SU000154
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:6 weeks old
Gender:Males
Species Group:Mammal

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
604 CecumSA007406FL001883Malegender
604 CecumSA007406FL001883Tcdf Treatedgenotype

Collection:

Collection ID:CO000138
Sample Type:Feces, liver, small and large intestine

Treatment:

Treatment ID:TR000156
Treatment Summary:Wild type and AHR knock out mice (5-6 per group) were treated with TCDF for 5 days
Treatment:Dietary
Treatment Compound:TCDF
Treatment Route:Oral
Treatment Dose:24 µg/kg body weight

Sample Preparation:

Sampleprep ID:SP000151
Sampleprep Protocol Filename:Bile_Acid_Extraction_for_LCMS.docx
Processing Method:Homogenization
Processing Storage Conditions:-20
Extraction Method:Ethanol extraction
Sample Spiking:Used D4 standards of Cholic Acid , Lithocholic Acid, Ursodeoxycholic Acid, Chenodeoxycholic Acid, and Deoxycholic Acid
Organ:Liver, small and large intestine, and feces

Combined analysis:

Analysis ID AN000217
Analysis type MS
Chromatography type
Chromatography system
Column
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Waters
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH000149

MS:

MS ID:MS000169
Analysis ID:AN000217
Instrument Name:Waters
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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