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MB Sample ID: SA014077

Local Sample ID:B6_F4
Subject ID:SU000331
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:[C57BL-6J (B6)]/[B6.Sle1.Sle2.Sle3 (TC)]
Age Or Age Range:99 days/93-99 days
Gender:female
Animal Animal Supplier:in house/in house
Animal Housing:SPF/SPF
Animal Light Cycle:standard|standard
Animal Feed:standard|standard
Animal Water:standard|standard
Species Group:Mammal

Select appropriate tab below to view additional metadata details:

Subject:

Subject ID:SU000331
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:[C57BL-6J (B6)]/[B6.Sle1.Sle2.Sle3 (TC)]
Age Or Age Range:99 days/93-99 days
Gender:female
Animal Animal Supplier:in house/in house
Animal Housing:SPF/SPF
Animal Light Cycle:standard|standard
Animal Feed:standard|standard
Animal Water:standard|standard
Species Group:Mammal

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
B6_F4SA014077FL003250C57BL/6JStrain
B6_F4SA014077FL003250controlTreatment

Collection:

Collection ID:CO000325
Collection Summary:None
Collection Protocol ID:IACUC 201308189
Collection Protocol Filename:Metabolomics_LCMSProtocol_plasma.pdf
Sample Type:blood
Collection Method:heart puncture
Collection Time:terminal
Storage Conditions:-20C
Collection Vials:heparinized tubes
Blood Serum Or Plasma:plasma

Treatment:

Treatment ID:TR000345
Treatment Summary:no treatement (controls for KD diet study)

Sample Preparation:

Sampleprep ID:SP000339
Sampleprep Summary:None
Sampleprep Protocol Filename:Metabolomics_LCMSProtocol_plasma.pdf
Appendix A - Internal Standard Prep GLCMS.pdf

Combined analysis:

Analysis ID AN000494 AN000495 AN000496 AN000497
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Peak Area Peak Area Peak height Peak height

Chromatography:

Chromatography ID:CH000351
Chromatography Summary:None
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS000432
Analysis ID:AN000494
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Positive Peak Area
Ion Mode:POSITIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000433
Analysis ID:AN000495
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Negative Peak Area
Ion Mode:NEGATIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000434
Analysis ID:AN000496
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Positive Peak Height
Ion Mode:POSITIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
  
MS ID:MS000435
Analysis ID:AN000497
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI Negative Peak Height
Ion Mode:NEGATIVE
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
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