Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA025991

Local Sample ID:	G341
Subject ID:	SU000523
Subject Type:	Animal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammals

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Subject:

Subject ID:	SU000523
Subject Type:	Animal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammals

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
G341	SA025991	FL006467	3.0	Radiation dose

Collection:

Collection ID:	CO000517
Collection Summary:	Plasma was prepared and snap-frozen at -80°C before NMR spectroscopy and morphology analysis.
Sample Type:	Blood

Treatment:

Treatment ID:	TR000537
Treatment Summary:	A total of 13 seven-week-old C57BL/6 female mice randomly into 3 groups, and then whole body being exposed to gamma radiation. The individually housed animals were fed a standard diet and maintained in an environment controlled facility with an ambient temperature of 22-25°C and 45% relative humidity on 12 h light-dark cycle. Mice were exposed to whole body gamma radiation using a high-activity source (1250 keV 60Co) with the linear energy transfer (LET) associated with these fields in the range of 0.2-2 keV/μm. The mice were isolated in the corner of their polymer cages and placed a minimum of 100 cm from the collimated 6000 Ci (222 TBq) 60Co source, and then irradiation to the proposed dosage, respectively. The result of absorbed dose rate at a depth of approximately 600 mg/cm2 were ~0.83 Gy/min relative to tissue. After irradiation the isolation barrier was removed and mice were transferred to Animal Facility. All procedures (i.e., placement in aerated boxes and subsequent tissue harvesting) for the control mice were identical to those of irradiated animals, except that they received no radiation.

Treatment ID:

TR000537

Treatment Summary:

A total of 13 seven-week-old C57BL/6 female mice randomly into 3 groups, and then whole body being exposed to gamma radiation. The individually housed animals were fed a standard diet and maintained in an environment controlled facility with an ambient temperature of 22-25°C and 45% relative humidity on 12 h light-dark cycle. Mice were exposed to whole body gamma radiation using a high-activity source (1250 keV 60Co) with the linear energy transfer (LET) associated with these fields in the range of 0.2-2 keV/μm. The mice were isolated in the corner of their polymer cages and placed a minimum of 100 cm from the collimated 6000 Ci (222 TBq) 60Co source, and then irradiation to the proposed dosage, respectively. The result of absorbed dose rate at a depth of approximately 600 mg/cm2 were ~0.83 Gy/min relative to tissue. After irradiation the isolation barrier was removed and mice were transferred to Animal Facility. All procedures (i.e., placement in aerated boxes and subsequent tissue harvesting) for the control mice were identical to those of irradiated animals, except that they received no radiation.

Sample Preparation:

Sampleprep ID:	SP000530
Sampleprep Summary:	Step 1: After randomization of the samples, every preweighted intact frozen plasma was added into MeOH and then vortex 2 mins. After centrifuge at 7200rpm for 5 minutes at 4C, the liqiuds layer in translted into a glass vial. Add 4 ml MeOH and 0.85 ml deionized H2O per gram of liver tissue, followed by vortexing the mixture and then 2 ml chloroform per gram of tissue was added and vortexed again. This process took 6 minutes and kept exactly the same for each sample. Step 2: 2 ml chloroform and 2 ml deionized H2O per gram of tissue were added in the mixture then vortexed again, followed by transferring the different layers into glass vials separately with syringes after the mixture (in ice bath) centrifuged. Finally, the solvents of hydrophilic metabolites were removed by employing lyophilizer and then stored at -80oC freezer before NMR spectral measurements.

Sampleprep ID:

SP000530

Sampleprep Summary:

Step 1: After randomization of the samples, every preweighted intact frozen plasma was added into MeOH and then vortex 2 mins. After centrifuge at 7200rpm for 5 minutes at 4C, the liqiuds layer in translted into a glass vial. Add 4 ml MeOH and 0.85 ml deionized H2O per gram of liver tissue, followed by vortexing the mixture and then 2 ml chloroform per gram of tissue was added and vortexed again. This process took 6 minutes and kept exactly the same for each sample. Step 2: 2 ml chloroform and 2 ml deionized H2O per gram of tissue were added in the mixture then vortexed again, followed by transferring the different layers into glass vials separately with syringes after the mixture (in ice bath) centrifuged. Finally, the solvents of hydrophilic metabolites were removed by employing lyophilizer and then stored at -80oC freezer before NMR spectral measurements.

Analysis:

MB Sample ID:	SA025991
Analysis ID:	AN000770
Analysis Type:	NMR
Num Factors:	3
Num Metabolites:	42
Units:	µM/mg

NMR:

NMR ID:	NM000090
Analysis ID:	AN000770
Instrument Name:	Varian INOVA 800
Instrument Type:	FT-NMR
NMR Experiment Type:	1D 1H
Spectrometer Frequency:	800 MHz