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MB Sample ID: SA027995

Local Sample ID:213269
Subject ID:SU000563
Subject Type:Animal
Subject Species:Canis lupus familiaris
Taxonomy ID:9615
Age Or Age Range:1 year - 15 years
Weight Or Weight Range:5 kg - 40 kg
Gender:Male Neutered or Female Spayed
Species Group:Mammal

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Subject:

Subject ID:SU000563
Subject Type:Animal
Subject Species:Canis lupus familiaris
Taxonomy ID:9615
Age Or Age Range:1 year - 15 years
Weight Or Weight Range:5 kg - 40 kg
Gender:Male Neutered or Female Spayed
Species Group:Mammal

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
213269SA027995FL006937DiabeticHealth Status
213269SA027995FL006937Neutered maleGender
213269SA027995FL006937FastedFasting Status
213269SA027995FL006937Labrador retrieverBreed
213269SA027995FL006937LargeSize (S/M/L)

Collection:

Collection ID:CO000557
Collection Summary:Blood collected by peripheral venipuncture into red top tube (containing clot activator). Blood was allowed to clot, then centrifuged at 2000 g x 7 minutes between 20-30 minutes after sample collection. Serum was frozen immediately after separation (always within 1 hour of collection) at -80 C.
Sample Type:Blood (serum)
Collection Method:Peripheral venipuncture
Collection Location:Jugular, saphenous, or cephalic vein
Collection Frequency:Once
Storage Conditions:Room temperature until centrifugation. Then -80 C immediately after separation from red cells. Serum was thawed once on ice, 100 uL aliquots taken, and aliquots re-frozen (at -80C) until use for metabolomics.
Collection Vials:Red top with clot activator
Storage Vials:Cryovials (polypropylene with silicone gasket - Fisher Brand)
Collection Tube Temp:Room temperature
Blood Serum Or Plasma:Serum

Treatment:

Treatment ID:TR000577
Treatment Summary:None

Sample Preparation:

Sampleprep ID:SP000570
Sampleprep Summary:See protocol
Sampleprep Protocol Filename:Metabolomics_LCMSProtocol_plasma.pdf

Combined analysis:

Analysis ID AN000822 AN000823
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific Q Exactive Thermo Scientific Q Exactive
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak Area Peak Area

Chromatography:

Chromatography ID:CH000587
Chromatography Summary:see protocol
Instrument Name:Thermo Scientific Q Exactive
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000723
Analysis ID:AN000822
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000724
Analysis ID:AN000823
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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