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MB Sample ID: SA075525
| Local Sample ID: | F_728_138 |
| Subject ID: | SU001154 |
| Subject Type: | Mammal |
| Subject Species: | Ovis aries |
| Taxonomy ID: | 9940 |
| Age Or Age Range: | Gestational days 125-birth |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001154 |
| Subject Type: | Mammal |
| Subject Species: | Ovis aries |
| Taxonomy ID: | 9940 |
| Age Or Age Range: | Gestational days 125-birth |
| Species Group: | Mammals |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| F_728_138 | SA075525 | FL011653 | Control | Treatment |
| F_728_138 | SA075525 | FL011653 | Fetal | Source |
Collection:
| Collection ID: | CO001148 |
| Collection Summary: | Blood samples were collected from ewe and fetus on gestational days 125, 130, 135, 138, 140, and every subsequent day until birth (control: 144±1; CORT: 142±2 days gestational age); |
| Sample Type: | Blood (serum) |
| Collection Method: | samples for metabolomics analysis were collected in glass tubes on ice with no anticoagulant and spun for 20 min at 4 °C; the resulting serum was stored at -20 °C until metabolomic analysis. |
| Collection Location: | University of Florida |
| Storage Conditions: | -20℃ |
| Collection Vials: | Eppendorf tubes |
| Storage Vials: | Eppendorf tubes |
Treatment:
| Treatment ID: | TR001168 |
| Treatment Summary: | The treatment protocol for this study was previous published in Antolic, A., et al. (2018). Chronic maternal hypercortisolemia in late gestation alters fetal cardiac function at birth. Am J Physiol Regul Integr Comp Physiol 314(3): R342-R352. |
| Treatment Compound: | Hydrocortisone sodium succinate in sodium phosphate (Solu-Cortef; Pfizer, New York, NY, USA) |
| Treatment Dose: | 1 mg/kg/day |
Sample Preparation:
| Sampleprep ID: | SP001161 |
| Sampleprep Summary: | Saline buffer was created by weighing 9 g of NaCl into a 1 L volumetric flask. Next, 100 mL of D2O was added and filled up to 1 L with water and shaken until dissolved. Samples were thawed on ice and centrifuged at 3,000 g for 20 min at 4 °C. Two hundred mL of serum was mixed with 400 mL of Saline buffer and centrifuged for 10 min at 3000 g at 4 °C. For each sample, 500 mL of the resulting supernatant was transferred into a 5 mm SampleJet NMR tube (Bruker Biospin, Billerica, MA, USA). |
Analysis:
| MB Sample ID: | SA075525 |
| Analysis ID: | AN001803 |
| Laboratory Name: | Advanced Magnetic Resonance Imaging and Spectroscopy Facility-University of Florida |
| Analysis Type: | NMR |
| Analysis Protocol File: | Serum_CORT_raw_data_cpmg.zip |
| Acquisition Parameters File: | Serum_CORT_raw_data_cpmg.zip |
| Software Version: | Topspin 3.6 |
| Operator Name: | Jacquelyn Walejko |
| Processing Parameters File: | Serum_CORT_raw_data_cpmg.zip |
| Num Factors: | 4 |
| Num Metabolites: | 29 |
| Units: | Area under curve |
NMR:
| NMR ID: | NM000139 |
| Analysis ID: | AN001803 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Field Frequency Lock: | H2O+D2O |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | 5mm Cryoprobe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5mm |
| Shimming Method: | Topshim |
| Pulse Sequence: | cpmgpr1d |
| Water Suppression: | Presaturation |
| Power Level: | 7.37 W |
| Receiver Gain: | 64 |
| Offset Frequency: | 2824.2 |
| Presaturation Power Level: | 6.41E-05 |
| Temperature: | 4C |
| Number Of Scans: | 128 |
| Dummy Scans: | 16 |
| Acquisition Time: | 1.8173 |
| Relaxation Delay: | 4 sec |
| Spectral Width: | 15.0182 ppm |
| Num Data Points Acquired: | 32764 |
| Real Data Points: | 65536 |
| Line Broadening: | 1 |
