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MB Sample ID: SA078245
| Local Sample ID: | Vehicle-5 |
| Subject ID: | SU001197 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Genotype Strain: | ZDF rats |
| Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001197 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Genotype Strain: | ZDF rats |
| Gender: | Not applicable |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Vehicle-5 | SA078245 | FL011921 | 17-week-old ZDF rat | Genotype |
| Vehicle-5 | SA078245 | FL011921 | - | Treatment |
| Vehicle-5 | SA078245 | FL011921 | 6 | Duration (weeks) |
Collection:
| Collection ID: | CO001191 |
| Collection Summary: | After euthanasia by exsanguination under isoflurane anesthesia, a piece of liver was taken from the left lateral lobe on the treated rats, weighed at approximately 50mg, and immediately frozen in liquid nitrogen. |
| Sample Type: | Liver |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001212 |
| Treatment Summary: | The baseline control, the liver samples of ZDF rats were taken from the baseline control group of 11-week-old ZDF rats. And at the same time, the other 2 comparing groups were started on oral administration of OPC-163493 or vehicle solution for 6-weeks. After 6-week dosing, liver samples were taken from both groups, and the liver metabolites of all three groups including the baseline controls were analyzed. |
| Treatment Dose: | 0mg/kg/day, 6mg/kg/day |
Sample Preparation:
| Sampleprep ID: | SP001205 |
| Sampleprep Summary: | The frozen liver samples were plunged into 50% acetonitrile/Milli-Q water containing internal standard. The sample was homogenized and then centrifuged. Subsequently, 800 uL of upper aqueous layer was filtered through a 5-kDa cutoff filter. The filtrate was centrifugally concentrated and re-suspended in 50 uL of Milli-Q water. |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 50 uL Mili-Q |
Combined analysis:
| Analysis ID | AN001862 | AN001863 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent 7100 CE | Agilent 7100 CE |
| Column | Fused silica capillary, i.d. 50 μm × 80 cm | Fused silica capillary, i.d. 50 μm × 80 cm |
| MS Type | ESI | ESI |
| MS instrument type | Other | Triple quadrupole |
| MS instrument name | Agilent 6210 TOF | Agilent 6460 QQQ |
| Ion Mode | UNSPECIFIED | UNSPECIFIED |
| Units | Concentration (nmol/g tissue) | Concentration (nmol/g tissue) |
Chromatography:
| Chromatography ID: | CH001348 |
| Chromatography Summary: | capillary electrophoresis was connected with time-of-flight mass spectrometry (CE-TOFMS) for cation analysis and tandem mass spectrometry (CE-MS/MS) for anion. |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | Fused silica capillary, i.d. 50 μm × 80 cm |
| Chromatography Type: | CE |
MS:
| MS ID: | MS001722 |
| Analysis ID: | AN001862 |
| Instrument Name: | Agilent 6210 TOF |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | The spectrometer was scanned from m/z 50 to 1,000. Peaks were extracted using automatic integration software MasterHands (Keio University, Tsuruoka, Japan) in order to obtain peak information including m/z, migration time for CE-TOFMS measurement (MT) and peak area. Signal peaks corresponding to isotopomers, adduct ions, and other product ions of known metabolites were excluded, and remaining peaks were annotated with putative metabolites from the HMT metabolite database based on their MTs and m/z values determined by TOFMS. The tolerance range for the peak annotation was configured at ±0.5 min for MT and ±10 ppm for m/z. In addition, peak areas were normalized against those of the internal standards and then the resultant relative area values were further normalized by sample amount. |
| Ion Mode: | UNSPECIFIED |
| MS ID: | MS001723 |
| Analysis ID: | AN001863 |
| Instrument Name: | Agilent 6460 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | The spectrometer was scanned from m/z 50 to 1,000. Peaks were extracted using MasterHands, automatic integration software (Keio University, Tsuruoka, Yamagata, Japan) and MassHunter Quantitative Analysis B.04.00 (Agilent Technologies) in order to obtain peak information including m/z, peak area, and migration time (MT). Signal peaks were annotated according to the HMT metabolite database based on their m/z values with the MTs. The peak area of each metabolite was normalized with respect to the area of the internal standard and metabolite concentration was evaluated by standard curves with three-point calibrations using each standard compound. |
| Ion Mode: | UNSPECIFIED |
