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MB Sample ID: SA095237
| Local Sample ID: | datg3_30 Rep 3_008 |
| Subject ID: | SU001392 |
| Subject Type: | Other |
| Subject Species: | Saccharomyces cerevisiae |
| Taxonomy ID: | 4932 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001392 |
| Subject Type: | Other |
| Subject Species: | Saccharomyces cerevisiae |
| Taxonomy ID: | 4932 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| datg3_30 Rep 3_008 | SA095237 | FL013539 | 2 - datg3_30 | Treatment |
Collection:
| Collection ID: | CO001387 |
| Collection Summary: | Yeast cells (WT and mutants of datg3 and datg32 were grown at 30 or 37 degrees in YPGE media to logarithmic phase, quenched with -20 chilled metanol:water (3:2), spun at 1000g for 5 minutes and the supernatant was discarded. Pellets were then saved at -80. |
| Sample Type: | Yeast cells |
Treatment:
| Treatment ID: | TR001407 |
| Treatment Summary: | Three genotypes- WT, atg3 null and atg32 null cells were grown at 30 or 37 degree for each group. (Total 6 samples per treatment). |
Sample Preparation:
| Sampleprep ID: | SP001400 |
| Sampleprep Summary: | Added 500 uL 3:3:2 and vortex before transferring to 2 mL eppendorf tube. Added another 500 uL 3:3:2 to sample tubes and vortexed before transferring to 2 mL eppendorf tube. |
Combined analysis:
| Analysis ID | AN002194 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus III GC TOF |
| Ion Mode | POSITIVE |
| Units | normalized peak height |
Chromatography:
| Chromatography ID: | CH001609 |
| Instrument Name: | Agilent 6890N |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002041 |
| Analysis ID: | AN002194 |
| Instrument Name: | Leco Pegasus III GC TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| MS Comments: | Raw files are processed directly after data acquisition and stored as ChromaTOF-specific. |
| Ion Mode: | POSITIVE |
