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MB Sample ID: SA100901

Local Sample ID:Sample_3
Subject ID:SU001454
Subject Type:Other
Subject Species:Homo sapiens
Taxonomy ID:9606

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Subject:

Subject ID:SU001454
Subject Type:Other
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
Sample_3SA100901FL014195SerumTreatment

Collection:

Collection ID:CO001449
Collection Summary:FI-MS method optimization was performed with commercially available serum, Human AB Serum (Biological Industries USA, Inc., USA). Matrix effect experiments and biological applications were performed with 98 serum samples of healthy individuals obtained from Rambam Hospital, Haifa, Israel (IRB 0481-18-RMB).
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR001469
Treatment Summary:To extract metabolites and lipids from serum samples, we mixed 20 µL of serum with an extraction solution for metabolomics analysis and 10 µL for lipidomics in 96-deep well plates. For lipidomics analysis, we utilized 100 µL of 2-propanol/methanol (6:1, v/v); and for metabolomics analysis, 100 µL of methanol/acetonitrile/water (5:3:1, v/v/v). After 10 min of vortexing, 800 rpm, precipitated proteins were separated by centrifugation for 20 min at 4 °C and 4000 rcf; supernatants were stored at -80 °C prior the analysis.

Sample Preparation:

Sampleprep ID:SP001462
Sampleprep Summary:To extract metabolites and lipids from serum samples, we mixed 20 µL of serum with an extraction solution for metabolomics analysis and 10 µL for lipidomics in 96-deep well plates. For lipidomics analysis, we utilized 100 µL of 2-propanol/methanol (6:1, v/v); and for metabolomics analysis, 100 µL of methanol/acetonitrile/water (5:3:1, v/v/v). After 10 min of vortexing, 800 rpm, precipitated proteins were separated by centrifugation for 20 min at 4 °C and 4000 rcf; supernatants were stored at -80 °C prior the analysis.

Combined analysis:

Analysis ID AN002300
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system none
Column none
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode UNSPECIFIED
Units intensity

Chromatography:

Chromatography ID:CH001690
Instrument Name:none
Column Name:none
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS002143
Analysis ID:AN002300
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:To determine the number of reproducibly detected m/z features by a specific FI-MS method configuration, we performed 6 repeated injections of the biological sample from the same vial followed by the injection of 6 blank samples (i.e. sample preparation protocol applied to a water sample) and identified reproducibly detected features based on low RSD (<30%) and high SNR (>4)
Ion Mode:UNSPECIFIED
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