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MB Sample ID: SA151747

Local Sample ID:11_D6_DT1
Subject ID:SU001729
Subject Type:Cultured cells
Subject Species:Plasmodium falciparum
Taxonomy ID:5833

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Subject:

Subject ID:SU001729
Subject Type:Cultured cells
Subject Species:Plasmodium falciparum
Taxonomy ID:5833

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
11_D6_DT1SA151747FL017816Wild-typeGenotype
11_D6_DT1SA151747FL017816DSM-1Treatment

Collection:

Collection ID:CO001722
Collection Summary:Cells were pelleted and the media aspirated prior to methanol extraction
Sample Type:Plasmodium cells

Treatment:

Treatment ID:TR001742
Treatment Summary:Trophozoite-stage Plasmodium infected RBCs were treated with 10x IC50 of either drug for 2.5 hours

Sample Preparation:

Sampleprep ID:SP001735
Sampleprep Summary:90% Methanol/10% water at 50:1 solvent/cell pellet followed by vortexing and clarification via centrifugation
Processing Method:Lysis
Processing Storage Conditions:4℃
Extract Cleanup:Clarification via centrifugation followed by nitrogen drying
Extract Storage:-80℃
Sample Resuspension:6X pellet volume of HPLC H2O
Sample Spiking:Internal Standard 13C4, 15N1-Aspartate used for sample preparation normalizer

Combined analysis:

Analysis ID AN002699
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Exactive Orbitrap Plus
Column Phenomenex Synergi Hydro RP (100 x 2mm,2.5um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode NEGATIVE
Units intensity

Chromatography:

Chromatography ID:CH001991
Chromatography Summary:Low pH polar
Instrument Name:Thermo Exactive Orbitrap Plus
Column Name:Phenomenex Synergi Hydro RP (100 x 2mm,2.5um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002497
Analysis ID:AN002699
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Proprietary analytical software for integration and peak picking
Ion Mode:NEGATIVE
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