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MB Sample ID: SA174241

Local Sample ID:a4
Subject ID:SU001933
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6
Age Or Age Range:8 weeks
Weight Or Weight Range:20-25 g
Gender:Male
Animal Animal Supplier:Model Animal Research Center of Nanjing University
Animal Housing:Standard specific pathogen free (SPF) conditions
Animal Light Cycle:12:12-h light/dark cycle
Animal Feed:food freely
Animal Water:water freely

Select appropriate tab below to view additional metadata details:


Subject:

Subject ID:SU001933
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6
Age Or Age Range:8 weeks
Weight Or Weight Range:20-25 g
Gender:Male
Animal Animal Supplier:Model Animal Research Center of Nanjing University
Animal Housing:Standard specific pathogen free (SPF) conditions
Animal Light Cycle:12:12-h light/dark cycle
Animal Feed:food freely
Animal Water:water freely

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
a4SA174241FL021375shamsurgery
a4SA174241FL021375-FG-4592(10 mg/kg)

Collection:

Collection ID:CO001926
Collection Summary:Non-target metabolomics analysis was carried out using the kidney tissues of mice sacrificed at day 10 after UIR.
Sample Type:Kidney

Treatment:

Treatment ID:TR001945
Treatment Summary:C57BL/6 mice were anesthetized using isoflurane. UIR was induced by clamping the right renal pedicle for 45 minutes and then releasing it to allow reperfusion, leaving the left kidney intact. Sham treated mice served as controls. Each mouse was located supine on a thermostatic pad (37 °C) to maintain its body temperature throughout the whole process. After 3 days of recovery, the mice received a daily intraperitoneal (i.p.) injection of FG4592 (10 mg/kg) or vehicle for 7 consecutive days. After treatment, the mice were sacrificed. Non-target metabolomics analysis was carried out using the kidney tissues of mice sacrificed at day 10 after UIR.
Treatment Compound:FG4592
Treatment Dosevolume:10 mg/kg

Sample Preparation:

Sampleprep ID:SP001939
Sampleprep Summary:20 mg of each tissue sample were homogenized with 0.6 mL of 2 chlorophenylalanine (4 ppm) containing methanol (−20 °C), centrifuged, and filtered through a 0.22-μm membrane.

Combined analysis:

Analysis ID AN003008 AN003009
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Ultimate 3000 system Thermo Ultimate 3000 system
Column Waters ACQUITY UPLC HSS T3 Waters ACQUITY UPLC HSS T3
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units AUC AUC

Chromatography:

Chromatography ID:CH002230
Chromatography Summary:A 2 μL aliquot of the samples was injected into a Thermo Ultimate 3000 system equipped with an ACQUITY UPLC® HSS T3 column (150 × 2.1 mm, 1.8 μm, Waters, Milford, MA, USA) maintained at 40 °C. The mobile phase for positive mode analysis consists of 0.1% formic acid in water (phase C) and 0.1% formic acid in acetonitrile (phase D), while the mobile phase for negative mode analysis consists of 5 mM ammonium formate in water (phase A) and acetonitrile (phase B). Phase C and D, as well as phase A and B, were mixed and delivered at a flow rate of 0.25 mL/min with a gradient program as follows: 0~1 min; 2% B/D; 1~9 min, 2%~50% B/D; 9~12 min, 50%~98% B/D; 12~13.5 min, 98% B/D; 13.5~14 min, 98%~2% B/D; 14~20 min, 2% D-positive model (14~17 min, 2% B-negative model).
Instrument Name:Thermo Ultimate 3000 system
Column Name:Waters ACQUITY UPLC HSS T3
Column Temperature:40
Flow Gradient:phase A and B were mixed and delivered at a flow rate of 0.25 mL/min with a gradient program as follows: 0-1 min; 2% A/B; 1-9 min, 2%-50% A/B; 9-12 min, 50%-98% A/B; 12-13.5 min, 98% A/B; 13.5-14 min, 98%-2% A/B; 14-20 min, 2% B-positive model (14-17 min, 2% B-negative model)
Flow Rate:0.25ml/min
Solvent A:100% water; 5mM ammonium formate
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS002797
Analysis ID:AN003008
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Proteowizard software (v3.0.8789)
Ion Mode:POSITIVE
  
MS ID:MS002798
Analysis ID:AN003009
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Proteowizard software (v3.0.8789)
Ion Mode:NEGATIVE
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