Return to study ST001927 main page
MB Sample ID: SA178417
| Local Sample ID: | Consortium_3 |
| Subject ID: | SU002005 |
| Subject Type: | Fungi |
| Subject Species: | Beauveria bassiana |
| Genotype Strain: | GenBank: KU751847; KU751848 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002005 |
| Subject Type: | Fungi |
| Subject Species: | Beauveria bassiana |
| Genotype Strain: | GenBank: KU751847; KU751848 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Consortium_3 | SA178417 | FL022206 | Consortium formed by Bov2 and Bov3 strains | Fungal species |
Collection:
| Collection ID: | CO001998 |
| Collection Summary: | Two genetically distinct strains of Beauveria bassiana (Bov 3 and Bov 2) were cultivated both separately and co-cultivated to form a fungal consortium. After the colonies had grown, the mycelium of each treatment (Bov 2, Bov 3, and the fungal consortium) was scraped from the culture medium with a spatula and then macerated separately in liquid nitrogen (N2) |
| Collection Protocol Filename: | MetaboliteExtraction |
| Sample Type: | Fungal mycelium |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002017 |
| Treatment Summary: | Two genetically distinct strains of B. bassiana (Bov 3 and Bov 2) were cultivated in Petri dishes containing Agar Sabouraud culture medium, both separately and co-cultivated to form a fungal consortium. The cultures were incubated in the dark in a biological oxygen demand (BOD) oven for 14 days at 28°C. |
Sample Preparation:
| Sampleprep ID: | SP002011 |
| Sampleprep Summary: | Extraction was performed in microtubes, from 200 mg of fungal macerate to which 1 mL of 6:2:2 methanol:chloroform:water ice-cold extraction solution was added. These extraction microtubes were vigorously vortexed and placed in an ultrasonic low-temperature bath at 20 Hz s-1 for 15 min. The samples were then centrifuged (Eppendorf, Germany) at 4°C for 10 min at 14,000 rpm. Then, the supernatant was filtered using a 0.22 μm Whatman® filter (Merck, Germany) and transferred to a chromatographic vial where the extracts were lyophilized (Thermo Fischer Scientific, MA, USA) until completely dry. Finally, the lyophilized samples were resuspended in 200 μL of extraction solution and aliquoted for use in the GC-MS and LC-MS/MS. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN003133 | AN003134 | AN003135 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | GC | Reversed phase | Reversed phase |
| Chromatography system | Agilent 7890A | Waters Acquity UPLC | Waters Acquity UPLC |
| Column | Agilent DB-5 (20m x 0.18mm, 0.18um); Restek RX-T 17 (0.9m x 0.10mm, 0.10um) | Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) | Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) |
| MS Type | EI | ESI | ESI |
| MS instrument type | GC x GC-TOF | QTOF | QTOF |
| MS instrument name | Leco Pegasus 4D GCxGC TOF | Waters Ultima QTOF | Waters Ultima QTOF |
| Ion Mode | UNSPECIFIED | POSITIVE | NEGATIVE |
| Units | Relative intensity | Relative intensity |
Chromatography:
| Chromatography ID: | CH002315 |
| Chromatography Summary: | (.cdf) |
| Methods Filename: | LCMSMS |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB-5 (20m x 0.18mm, 0.18um); Restek RX-T 17 (0.9m x 0.10mm, 0.10um) |
| Column Temperature: | 70 - 320 ºC |
| Flow Rate: | 1 mL.min-1 |
| Injection Temperature: | 280 ºC |
| Sample Injection: | 1 uL |
| Oven Temperature: | 70°C for 2 min, increasing by 15°C·min-1 until it reached 320°C and then held at this temperature for 4 min. |
| Chromatography Type: | GC |
| Chromatography ID: | CH002316 |
| Chromatography Summary: | Positive Mode (.raw) |
| Methods Filename: | LCMSMS |
| Instrument Name: | Waters Acquity UPLC |
| Column Name: | Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) |
| Column Temperature: | 35 ºC |
| Flow Gradient: | 95% solvent A and 5% B. The gradient increased linearly to 75% A and 25% B over the next 6 min. The polarity was reversed to 25% A and 75% B for 6 min, and finally 5% A and 95% B for 1 min |
| Flow Rate: | 0.5 mL·min-1 |
| Solvent A: | Water; formic acid |
| Solvent B: | 100% acetonitrile; formic acid. |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002317 |
| Chromatography Summary: | Negative mode (.raw) |
| Methods Filename: | LCMSMS |
| Instrument Name: | Waters Acquity UPLC |
| Column Name: | Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) |
| Column Temperature: | 35 ºC |
| Flow Gradient: | 95% solvent A and 5% B. The gradient increased linearly to 75% A and 25% B over the next 6 min. The polarity was reversed to 25% A and 75% B for 6 min, and finally 5% A and 95% B for 1 min |
| Flow Rate: | 0.5 mL·min-1 |
| Solvent A: | Water; formic acid |
| Solvent B: | 100% acetonitrile; formic acid. |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002913 |
| Analysis ID: | AN003133 |
| Instrument Name: | Leco Pegasus 4D GCxGC TOF |
| Instrument Type: | GC x GC-TOF |
| MS Type: | EI |
| MS Comments: | Data from GC-MS was processed using ChromaTOF 4.32 software. NIST library version 11 was used for the identification of metabolites. |
| Ion Mode: | UNSPECIFIED |
| Ion Source Temperature: | 250 ºC |
| Ionization Energy: | 70 eV |
| MS ID: | MS002914 |
| Analysis ID: | AN003134 |
| Instrument Name: | Waters Ultima QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Generated data were pre-processed using MassLynx 4.1 software |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 3 kV |
| Source Temperature: | 150 ºC |
| Desolvation Gas Flow: | 550 L/hr. |
| MS ID: | MS002915 |
| Analysis ID: | AN003135 |
| Instrument Name: | Waters Ultima QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Generated data were pre-processed using MassLynx 4.1 software |
| Ion Mode: | NEGATIVE |
| Capillary Voltage: | 3 kV |
| Source Temperature: | 150 ºC |
| Desolvation Gas Flow: | 550 L/hr. |
