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MB Sample ID: SA203024

Local Sample ID:SN-CIS-48h-3
Subject ID:SU002201
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Cell Strain Details:A2780 ovarian cancer cells

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Subject:

Subject ID:SU002201
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Cell Strain Details:A2780 ovarian cancer cells

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
SN-CIS-48h-3SA203024FL024990mediumsample_type
SN-CIS-48h-3SA203024FL024990cisplatintreatment_type
SN-CIS-48h-3SA203024FL02499048treatment_time
SN-CIS-48h-3SA203024FL024990A2780cell_line

Collection:

Collection ID:CO002194
Collection Summary:A2780 cells were purchase from Sigma-Aldrich and derived from the European Collection of Authenticated Cell Culture (human ovarian carcinoma; catalogue no.: 93112519; lot no.13J012, passage no.: P + 9). A2780cp cells were kindly provided by Dr Simona Bracini and were obtained via prolonged exposition to sub-lethal dose of cisplatin. Cells were maintained in RPMI1640 medium supplemented with 2 mM glutamine, 10% of FCS and antibiotics at 37 °C in a 5% CO2 atmosphere and sub-cultured twice weekly. Split 1:5 (3–6 × 10^4 cells per mL).
Sample Type:Cultured cells
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002213
Treatment Summary:For drug treatment, 2.7 *10^6 A2780 cells were seeded in p100 plate and after 24 hours were exposed to a concentration of the three Pt-drugs, i.e. CIS, OXA and CARBO equal to their 72 h-exposure IC50 value (2.5, 55 and 0.46 uM, respectively). The drug incubations were stopped at two different time points, 24 h and 48 h. The A2780cp cells were exposed to a concentration of cisplatin equal to their 72 h-exposure IC50 value (15 uM). The drug incubation was stopped after 48 h of treatment.
Treatment:Cisplatin, carboplatin and oxaliplatin
Cell Media:RPMI1640

Sample Preparation:

Sampleprep ID:SP002207
Sampleprep Summary:Frozen samples were thawed at room temperature and shaken before use. For cell lysates, 50 μl of 2H2O were added to 450 μl of each lysate sample. In the case of cell culture media, 300 μl of sodium phosphate buffer (70 mM Na2HPO4; 20% v/v 2H2O; 4.6 mM TMSP, pH 7.4) was added to 300 μl of each medium sample. The mixtures were homogenized by vortexing for 30 s and transferred into 5 mm NMR tubes (Bruker BioSpin srl) for analysis.

Analysis:

MB Sample ID:SA203024
Analysis ID:AN003464
Laboratory Name:Metabolomics
Analysis Type:NMR
Results File:ST002116_AN003464_Results.txt
Units:ppm

NMR:

NMR ID:NM000235
Analysis ID:AN003464
Instrument Name:Bruker Advance 600 MHZ
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:600 MHz
NMR Tube Size:5,00 mm
Water Suppression:water presaturation
Temperature:300 K for lysates and 31o for media
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