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MB Sample ID: SA211590
| Local Sample ID: | Saliva C12-01-5569 |
| Subject ID: | SU002297 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 17-25 |
| Gender: | Male |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002297 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 17-25 |
| Gender: | Male |
| Species Group: | Mammals |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Saliva C12-01-5569 | SA211590 | FL025804 | Non-smoker | Treatment |
Collection:
| Collection ID: | CO002290 |
| Collection Summary: | Saliva samples were collected from 30 non smokers and 30 smokers. Participants were instructed to observe a two-hour fast without eating, smoking or drinking followed by a thorough rinsing of the mouth to presvent any sample contamination. Subjects were instructed to allow saliva to pool in the bottom of the mouth and drain it into sterile collection tubes. The samples were transferred to the lab in ice containers and centrifuged at 2500 rpm for 5 minutes at 4°C to remove cell debris, and the supernatants stored at 80°C until analysis. |
| Sample Type: | Saliva |
Treatment:
| Treatment ID: | TR002309 |
| Treatment Summary: | Saliva samples were collected from non smokers and smokers. Participants were instructed to observe a two-hour fast without eating, smoking or drinking followed by a thorough rinsing of the mouth to presvent any sample contamination. Subjects were instructed to allow saliva to pool in the bottom of the mouth and drain it into sterile collection tubes. The samples were transferred to the lab in ice containers and centrifuged at 2500 rpm for 5 minutes at 4°C to remove cell debris, and the supernatants stored at 80°C until analysis. |
Sample Preparation:
| Sampleprep ID: | SP002303 |
| Sampleprep Summary: | Protein was precipitated out via chloroform methanol precipitation and the metabolite containing supernatants taken forward for analysis. |
Combined analysis:
| Analysis ID | AN003615 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Bruker Elute |
| Column | Hamilton Intensity Solo 2 C18 |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | timsTOF |
| Ion Mode | POSITIVE |
| Units | AU |
Chromatography:
| Chromatography ID: | CH002670 |
| Chromatography Summary: | uHPLC C18 Reversed phase |
| Instrument Name: | Bruker Elute |
| Column Name: | Hamilton Intensity Solo 2 C18 |
| Column Temperature: | 35 |
| Flow Gradient: | 1%B to 99%B in 15 min |
| Flow Rate: | 250 uL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003366 |
| Analysis ID: | AN003615 |
| Instrument Name: | timsTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MS2 data were acquired in data-dependent acquisition using a fixed cycle time of 3s and using a scan range of 20 - 1,300 Metaboscape 4.0 was used for feature detection, retention time alignment, deisotoping and metabolite identification. |
| Ion Mode: | POSITIVE |
