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MB Sample ID: SA305625
| Local Sample ID: | Serum_Tglif-S_2m |
| Subject ID: | SU002933 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002933 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Serum_Tglif-S_2m | SA305625 | FL036716 | TgLC | Genotype |
| Serum_Tglif-S_2m | SA305625 | FL036716 | Fasted | Condition |
| Serum_Tglif-S_2m | SA305625 | FL036716 | polar | Metabolites |
Collection:
| Collection ID: | CO002926 |
| Collection Summary: | Whole blood was collected and centrifuged at 9000 rpm for 10 minutes. The supernatant is serum. Serum was then stored at -80 °C. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR002942 |
| Treatment Summary: | TgL and TgLC mice were injected with Tamoxifen under the fed and fasted conditions. |
Sample Preparation:
| Sampleprep ID: | SP002939 |
| Sampleprep Summary: | For lipidomic metabolites extraction, 10 μL serum were mixed with 75 μL methanol and 250 μL Methyl tert-butyl ether (MTBE). After shaking for 6 minutes at 4 °C, 62 μL H2O was added and followed by centrifugation for 2 minutes. Supernatants were dried down for further analysis. Polar metabolites were extracted from serum using the extraction buffer containing methanol: acetonitrile: H2O (40:40:20). |
Combined analysis:
| Analysis ID | AN004605 | AN004606 | AN004607 | AN004608 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | NEGATIVE | POSITIVE | NEGATIVE | POSITIVE |
| Units | Ion counts | Ion counts | Ion counts | Ion counts |
Chromatography:
| Chromatography ID: | CH003465 |
| Chromatography Summary: | Polar metabolite analysis |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 25C |
| Flow Gradient: | 0 min, 100% B; 3 min, 100% B; 3.2 min, 90% B; 6.2 min, 90% B; 6.5 min, 80% B; 10.5 min, 80% B; 10.7 min, 70% B; 13.5 min, 70% B; 13.7 min, 45% B; 16 min, 45% B; 16.5 min, 100% B; and 22 min, 100% B |
| Flow Rate: | 0.3mL/min |
| Solvent A: | 95% water/5% acetonitrile; 20 mM acetic acid; 40 mM ammonium hydroxide, pH 9.4 |
| Solvent B: | 20% water/80% acetonitrile; 20 mM acetic acid; 40 mM ammonium hydroxide, pH 9.4 |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH003466 |
| Chromatography Summary: | Lipidomics method |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) |
| Column Temperature: | 55C |
| Flow Gradient: | 0 min, 25% B; 2 min, 25% B; 5.5 min, 65% B; 12.5 min, 100% B; 19.5 min, 100% B; 20.0 min, 25% B; 30 min, 25% B |
| Flow Rate: | 0.2mL/min |
| Solvent A: | 90% water/10% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate |
| Solvent B: | 75% isopropanol/25% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004351 |
| Analysis ID: | AN004605 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Negative Ionization mode |
| Ion Mode: | NEGATIVE |
| Capillary Temperature: | 300 |
| Ion Source Temperature: | 360 |
| Spray Voltage: | 2700 |
| Automatic Gain Control: | 3000000 |
| Acquisition Parameters File: | LCMS_Neg_22min_B_Vanquish |
| MS ID: | MS004352 |
| Analysis ID: | AN004606 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Positive Ionization mode |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 300 |
| Ion Source Temperature: | 360 |
| Spray Voltage: | 3500 |
| Automatic Gain Control: | 3000000 |
| Acquisition Parameters File: | LCMS_Pos_22min_B_Vanquish |
| MS ID: | MS004353 |
| Analysis ID: | AN004607 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Negative Ionization mode |
| Ion Mode: | NEGATIVE |
| Capillary Temperature: | 300 |
| Ion Source Temperature: | 360 |
| Spray Voltage: | 2700 |
| Automatic Gain Control: | 3000000 |
| Acquisition Parameters File: | LCMS_Neg_30min_55C_LongElution |
| MS ID: | MS004354 |
| Analysis ID: | AN004608 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Positive Ionization mode |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 300 |
| Ion Source Temperature: | 360 |
| Spray Voltage: | 3500 |
| Automatic Gain Control: | 3000000 |
| Acquisition Parameters File: | LCMS_Pos_30min_55C_LongElution |
