Return to study ST003063 main page
MB Sample ID: SA331965
| Local Sample ID: | serum-Con-4 |
| Subject ID: | SU003178 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003178 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| serum-Con-4 | SA331965 | FL039267 | Control | Genotype |
Collection:
| Collection ID: | CO003171 |
| Collection Summary: | Approximately 20 μL of blood was collected from the tail into tubes containing clotting activator (Starstedt Inc, 16.440.100). The blood samples were kept on ice and the serum was separated by centrifugation at 3,000 g for 10 min at 4 ˚C. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR003187 |
| Treatment Summary: | No treatment. All animal experiments conducted were performed in compliance of protocols approved by the Institutional Animal Care and Use Committee at Beth Israel Deaconess Medical Center (028-2022). All mice were housed under a 12 h – 12 h light/dark cycle. Room-temperature mice were housed at 23˚C in ventilated cages with an ACH of 25. |
Sample Preparation:
| Sampleprep ID: | SP003184 |
| Sampleprep Summary: | Serum metabolites were extracted using a 1:4 ratio of plasma to methanol extraction buffer. After centrifugation at 16,000 g for 15 minutes at 4°C, the supernatant was collected for LC-MS analysis. |
Combined analysis:
| Analysis ID | AN005018 |
|---|---|
| Chromatography ID | CH003791 |
| MS ID | MS004757 |
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1260 Infinity HPLC |
| Column | Waters Atlantis Silica HILIC (150 x 2.1mm, 3um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 4000 QTrap |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003791 |
| Instrument Name: | Agilent 1260 Infinity HPLC |
| Column Name: | Waters Atlantis Silica HILIC (150 x 2.1mm, 3um) |
| Column Temperature: | 25 °C |
| Flow Gradient: | 5% mobile phase A (10 mM ammonium formate and 0.1% formic acid in water) for 0.5 minute followed by a linear gradient to 40% mobile phase B (acetonitrile with 0.1% formic acid) over 10 minutes. |
| Flow Rate: | 250 µL/min |
| Solvent A: | 100% water; 10 mM ammonium formate and 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS004757 |
| Analysis ID: | AN005018 |
| Instrument Name: | ABI Sciex 4000 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | MS analyses are carried out using electrospray ionization in the positive ion mode using scheduled MRM method. Multiquant software (version 3.0.3, Sciex) was used for automatic peak integration followed by manual review of all peaks for quality of integration. |
| Ion Mode: | POSITIVE |
