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MB Sample ID: SA362652
| Local Sample ID: | UNMN-01562 |
| Subject ID: | SU003456 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL6/J; C57BL10/J; C57BL/10ScSn-Dmdmdx/J |
| Age Or Age Range: | 120-150 days |
| Weight Or Weight Range: | 25-35g |
| Height Or Height Range: | N/A |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratories |
| Animal Housing: | Research Animal Resources campus facilities |
| Animal Light Cycle: | 12h light/dark cycle |
| Animal Feed: | Standard chow |
| Animal Water: | Water bottle |
| Animal Inclusion Criteria: | Age; gender |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003456 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL6/J; C57BL10/J; C57BL/10ScSn-Dmdmdx/J |
| Age Or Age Range: | 120-150 days |
| Weight Or Weight Range: | 25-35g |
| Height Or Height Range: | N/A |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratories |
| Animal Housing: | Research Animal Resources campus facilities |
| Animal Light Cycle: | 12h light/dark cycle |
| Animal Feed: | Standard chow |
| Animal Water: | Water bottle |
| Animal Inclusion Criteria: | Age; gender |
| Species Group: | Mammals |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| UNMN-01562 | SA362652 | FL042224 | MTBD | GENOTYPE |
| UNMN-01562 | SA362652 | FL042224 | no stress | TREATMENT |
| UNMN-01562 | SA362652 | FL042224 | Blood plasma | Sample source |
Collection:
| Collection ID: | CO003449 |
| Collection Summary: | Blood was collected after euthanasia via cardiac puncture and plasma was extracted and stored at -80 degrees Celsius until sample submission to Metabolon. |
| Sample Type: | Blood (plasma) |
| Collection Method: | Blood was collected after euthanasia via cardiac puncture and was rotated end-over-end in EDTA-coated tubes for 15 minutes at room temperature. Samples were centrifuged for 10 minutes at 2,000xg at 4 degrees Celsius, then plasma supernatant was collected and stored at -80 degrees Celsius until sample submission to Metabolon. |
| Collection Location: | -80 degree freezer |
| Collection Frequency: | Single collection |
| Collection Duration: | 30 minutes |
| Volumeoramount Collected: | 200 microliters |
| Storage Conditions: | -80℃ |
| Collection Vials: | EDTA-coated centrifuge tubes |
| Storage Vials: | Centrifuge tubes |
| Collection Tube Temp: | 20-25 degrees Celsius |
| Additives: | EDTA |
Treatment:
| Treatment ID: | TR003465 |
| Treatment Summary: | Mice in the "no stress" group were euthanized and blood samples were collected following euthanasia. Mice in the "stress" group were monitored in open-field activity cages to measure total locomotion distance for 30 minutes, then were briefly handled by a standard scruff method for 30 seconds, followed by an additional 30 minute activity cage monitoring session and euthanasia/sample collection. |
| Treatment: | Scruff handling stress |
| Treatment Compound: | N/A |
| Treatment Route: | N/A |
| Treatment Dose: | N/A |
| Treatment Dosevolume: | N/A |
| Treatment Doseduration: | N/A |
| Treatment Vehicle: | N/A |
| Animal Vet Treatments: | N/A |
| Animal Anesthesia: | N/A |
| Animal Acclimation Duration: | N/A |
| Animal Fasting: | N/A |
| Animal Endp Euthanasia: | Tribromoethanol (Avertin) |
| Animal Endp Tissue Coll List: | Blood |
| Animal Endp Clinical Signs: | N/A |
Sample Preparation:
| Sampleprep ID: | SP003463 |
| Sampleprep Summary: | Plasma samples were pipetted in 200 microliter quantities into barcoded sample tubes and submitted to Metabolon for additional sample preparation and analysis. Sample preparation details are proprietary information held by Metabolon. |
| Processing Method: | N/A |
| Processing Storage Conditions: | On ice |
| Extraction Method: | N/A |
| Extract Enrichment: | N/A |
| Extract Cleanup: | N/A |
| Sample Resuspension: | N/A |
| Sample Derivatization: | N/A |
| Sample Spiking: | N/A |
| Subcellular Location: | N/A |
Combined analysis:
| Analysis ID | AN005464 | AN005465 | AN005466 | AN005467 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | HILIC |
| Chromatography system | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity |
| Column | Waters Acquity BEH C18 (100 x 2mm, 1.7um) | Waters Acquity BEH C18 (100 x 2mm, 1.7um) | Waters Acquity BEH C18 (100 x 2mm, 1.7um) | Waters Acquity BEH Amide (150 x 2.1mm, 1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | POSITIVE | NEGATIVE | NEGATIVE |
| Units | Peak Area | Peak Area | Peak Area | Peak Area |
Chromatography:
| Chromatography ID: | CH004149 |
| Chromatography Summary: | Low pH polar (LC/MS Pos early) |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm, 1.7um) |
| Column Temperature: | 40-50 |
| Flow Gradient: | Linear gradient from 5% B to 80% B over 3.35 minutes |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | 100% water; 0.1% formic acid; 0.05% PFPA, pH ~2.5 |
| Solvent B: | 100% methanol; 0.1% formic acid; 0.05% PFPA, pH ~2.5 |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH004150 |
| Chromatography Summary: | Low pH Lipophilic (LC/MS Pos late) |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm, 1.7um) |
| Column Temperature: | 40-50 |
| Flow Gradient: | Linear gradient from 40% B to 99.5% B over 1.0 minute, hold 99.5% B for 2.4 minutes. |
| Flow Rate: | 0.60 mL/min |
| Solvent A: | 100% water; 0.1% formic acid; 0.05% PFPA, pH ~2.5 |
| Solvent B: | 50% methanol/50% acetonitrile; 0.1% formic acid; 0.05% PFPA, pH ~2.5 |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH004151 |
| Chromatography Summary: | High pH (LC/MS Neg) |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm, 1.7um) |
| Column Temperature: | 40-50 |
| Flow Gradient: | Linear gradient from 0.5 to 70% B over 4.0 minutes, then rapid gradient to 99% B in 0.5 minutes. |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | 100% water; 6.5 mM ammonium bicarbonate, pH 8 |
| Solvent B: | 95% methanol/5% water; 6.5 mM ammonium bicarbonate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH004152 |
| Chromatography Summary: | HILIC (LC/MS Polar Neg) |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm, 1.7um) |
| Column Temperature: | 40-50 |
| Flow Gradient: | Linear gradient from 5% B to 50% B in 3.5 minutes, then linear gradient from 50% B to 95% B in 2 minutes. |
| Flow Rate: | 0.50 mL/min |
| Solvent A: | 15% water/5% methanol/80% acetonitrile; 10 mM ammonium formate, (effective pH 10.16 with NH4OH) |
| Solvent B: | 50% water/50% acetonitrile; 10 mM ammonium formate, (effective pH 10.60 with NH4OH) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS005190 |
| Analysis ID: | AN005464 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Metabolon (LC/MS Pos early) |
| Ion Mode: | POSITIVE |
| MS ID: | MS005191 |
| Analysis ID: | AN005465 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Metabolon (LC/MS Pos late) |
| Ion Mode: | POSITIVE |
| MS ID: | MS005192 |
| Analysis ID: | AN005466 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Metabolon (LC/MS Neg) |
| Ion Mode: | NEGATIVE |
| MS ID: | MS005193 |
| Analysis ID: | AN005467 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Metabolon (LC/MS Polar) |
| Ion Mode: | NEGATIVE |
