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MB Sample ID: SA385794
| Local Sample ID: | XiaChaoYi_A6_RP pos |
| Subject ID: | SU003633 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003633 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male |
| Species Group: | Mammals |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| XiaChaoYi_A6_RP pos | SA385794 | FL044542 | HT1080 human epithelial cancer cells | Sample source |
| XiaChaoYi_A6_RP pos | SA385794 | FL044542 | -CC-Met | Treatment |
Collection:
| Collection ID: | CO003626 |
| Collection Summary: | Cells were seeded at a density of 2×106 per well in a 10 cm glass bottom cell culture dish and grown for 12 h in DMEM supplemented with 10% Fetal Bovine Serum（FBS）, 1% Penicillin-Streptomycin(P/S). |
| Collection Protocol Filename: | 2024-NC.pdf |
| Sample Type: | HT1080 Human epithelial cancer cells |
Treatment:
| Treatment ID: | TR003642 |
| Treatment Summary: | HT1080 cells were cultured in the medium ± cystine or ± methionine as indicated for 10 h. The metabolites levels were measured by UHPLC-HRMS. |
Sample Preparation:
| Sampleprep ID: | SP003640 |
| Sampleprep Summary: | Cells were trypsinized, washed and resuspended in methanol: acetonitrile: ddH2O2 (2:2:1, v/v) after indicated treatment. Then subjected to LC/MS analysis at LipidALL Technologies (Changzhou, China). Briefly, polar metabolites were extracted using 1000 µl of ice-cold methanol: H2O (4:1, v/v), and incubated at 2000 × g for 30 min at 4oC. At the end of the incubation, samples were centrifuged for 10 min at 13,000 × g at 4oC. Clean supernatant was transferred to a new tube. Extracts were dried in a centrifugal concentrator. |
| Sampleprep Protocol Filename: | 2024-NC.pdf |
Combined analysis:
| Analysis ID | AN005752 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity II |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Triple TOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH004366 |
| Methods Filename: | 2024-NC.pdf |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0 - 1.0 min with 2% B, 1.0 - 6.0 min with 2% - 42% B, 6.0 - 8.0 min with 42% - 65% B, 8.0 - 10.0 min with 65% - 76% B, 10.0 - 11.0 min with 76% - 100% B, 11.0 - 14.0 min with 100% - 100% B. |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS005475 |
| Analysis ID: | AN005752 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| MS Comments: | MS parameters for detection were: ESI source voltage positive ion mode 5.5 kV, negative ion mode -4.5 kV; vaporizer temperature, 450°C; drying gas (N2) pressure, 50 psi; nebulizer gas (N2) pressure, 50 psi; curtain gas (N2) pressure, 35 psi; The scan range was m/z 60-800. Information-dependent acquisition mode was used for MS/MS analyses of the metabolites. Collision energy was set at (±) 35 ± 15 eV. Data acquisition and processing were performed using Analyst® TF 1.7.1 Software (AB Sciex, Concord, ON, Canada). |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | 2024-NC.pdf |
