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MB Sample ID: SA028731
| Local Sample ID: | Urine_UT_Rep6 |
| Subject ID: | SU000575 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000845 | AN000846 | AN000847 | AN000848 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
| Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) | EMD Millipore ZIC-HILIC (100 x 2.1mm,3.5um) | EMD Millipore ZIC-HILIC (100 x 2.1mm,3.5um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | QTOF | QTOF |
| MS instrument name | Agilent 6550 QTOF | Agilent 6550 QTOF | Agilent 6550 QTOF | Agilent 6550 QTOF |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Peak Area | Peak Area | Peak Area | Peak Area |
Chromatography:
| Chromatography ID: | CH000604 |
| Chromatography Summary: | Metabolites were separated using a C18 column, Zorbax Eclipse Plus C18 (2.1 x 100 mm, 1.8μm particle size), mobile phase A: water and mobile phase B: IPA/ACN(90:10) both with 0.1% HCOOH at a flow rate of 400μL/min injecting 2μL of extracts on a 1290 Infinity II LC System (Agilent Technologies). |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) |
| Flow Rate: | 400µL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000605 |
| Chromatography Summary: | Polar metabolites were analyzed using a EMD Millipore ZIC-HILIC (100 mm x 2.1 mm ID, 3.5μm) column, mobile phase A: water with 0.1% formic acid and B: Acetonitrile with 0.1% formic acid at a flow rate 300μL/min. 2μL of extract were injected on a 1290 Infinity II LC System (Agilent Technologies). For the negative ionization mode, mobile phase A was 10mM ammonium acetate and B was acetonitrile. |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | EMD Millipore ZIC-HILIC (100 x 2.1mm,3.5um) |
| Flow Rate: | 300µL/min |
| Solvent A: | Pos mode:100% water; 0.1% formic acid, Neg mode:100% water; 10 mM ammonium acetate |
| Solvent B: | Pos mode:100% acetonitrile; 0.1% formic acid, Neg mode:100% acetonitrile |
| Chromatography Type: | HILIC |
