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MB Sample ID: SA032599

Local Sample ID:ACC1-7
Subject ID:SU000614
Subject Type:Plant
Subject Species:Arabidopsis thaliana
Taxonomy ID:3702
Genotype Strain:Col-0
Age Or Age Range:12 Days after germination
Weight Or Weight Range:50 mg
Species Group:Plant

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Combined analysis:

Analysis ID AN000906
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1290
Column SeQuant ZIC-pHILIC (100 x 2.1mm,3.5um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH000643
Chromatography Summary:Samples were resolved using HILIC column
Instrument Name:Agilent 1290
Column Name:SeQuant ZIC-pHILIC (100 x 2.1mm,3.5um)
Column Temperature:30C
Flow Gradient:87% B for 5 minutes, decreased to 55% over 8 minutes and held for 4 minutes before returning to 87% over 3 minute
Flow Rate:0.3 ml min-1
Internal Standard:13C6 phenylalanine internal standard
Solvent A:100% water; 5 mM ammonium acetate, pH 4
Solvent B:90% acetonitrile/10% water; 0.1% formic acid
Chromatography Type:HILIC
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