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MB Sample ID: SA078180
| Local Sample ID: | C2 |
| Subject ID: | SU001195 |
| Subject Type: | Plant |
| Subject Species: | Brassica nigra |
| Taxonomy ID: | 3710 |
| Age Or Age Range: | 4 weeks old |
| Height Or Height Range: | 15-20 cm |
| Species Group: | Plants |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001859 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890 |
| Column | DB5-MS |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Agilent 6890 |
| Ion Mode | POSITIVE |
| Units | Normalized peak area |
Chromatography:
| Chromatography ID: | CH001346 |
| Chromatography Summary: | Samples were analysed by GC-TOF-MS (on an Agilent 6890 gas chromatograph equipped with a 10 m x 0.18 mm silica capillary column with a 0.18 µm DB 5-MS UI stationary phase, J&W Scientific; connected to a LECO Pegasus III TOF-MS) operated using the LECO ChromaTOF® software package (Leco Corp., St Joseph, MI, USA). Retention indices (RIs) were calculated relative to those of a C8-C40 alkane series. Deconvolution, feature extraction and peak integration were performed using a Matlab® script developed in-house. Identification was achieved by comparing compounds' RIs and mass spectra to the SMC in-house mass spectra library and to the public Golm Metabolome Database developed by the Max Planck Institute (GMD-MPI; http://gmd.mpimp-golm.mpg.de). Samples were normalized with respect to the peak areas of the internal standards (methyl stearate and SA-D4). |
| Instrument Name: | Agilent 6890 |
| Column Name: | DB5-MS |
| Flow Rate: | 1mL/min |
| Injection Temperature: | 270°C |
| Internal Standard: | salicylic acid |
| Internal Standard Mt: | stearic acid |
| Capillary Voltage: | 70-eV |
| Oven Temperature: | 70°C to 320°C (40 °C /min) |
| Running Voltage: | detector voltage 1,700V. |
| Transferline Temperature: | 250°C |
| Chromatography Type: | GC |
