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MB Sample ID: SA095501
Local Sample ID: | HeLa-wnt-60min-01 |
Subject ID: | SU001394 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002196 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | MS1 AUC integration |
Chromatography:
Chromatography ID: | CH001610 |
Chromatography Summary: | Samples were run on a Vanquish (Thermo Scientific) UHPLC system with mobile phase A (5mM NH4AcO, pH 9.9) and mobile phase B (ACN) at a flow rate of 200 µL/min on a Luna 3 um NH2 100A (150 × 2.0mm) at 40°C with a gradient going from 15% A to 95% A in 18 min followed by an 11 minute isocratic step. |
Instrument Name: | Thermo Vanquish |
Column Name: | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
Column Temperature: | 40 |
Flow Gradient: | 15% A to 95% A in 18 min followed by an 11 minute isocratic step |
Flow Rate: | 200 µL/min |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |