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MB Sample ID: SA100959
Local Sample ID: | WG350-4 |
Subject ID: | SU001456 |
Subject Type: | Bacteria |
Subject Species: | Escherichia coli |
Taxonomy ID: | 562 |
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Combined analysis:
Analysis ID | AN002303 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Accela 1250 |
Column | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | NEGATIVE |
Units | Peak Intensity |
Chromatography:
Chromatography ID: | CH001692 |
Chromatography Summary: | The acetonitrile:water extracts from parasites were dried under nitrogen flow and resuspended in 200 µls of water:methanol (97:3) containing 10 mM tributylamine and 15 mM acetic acid. A Thermo Accucore C18 column with a bed volume of 150 mm x 2.1 mm and 2.6 µ particle size was used. A solvent system composed of water buffered with 0.1 % formic acid (buffer A) and acetonitrile (buffer B), was used on a 20 minute gradient run with a flow rate of 200 µl/min as follows- hold at 10% acetonitrile for 30 seconds and gradually ramp up to 15%, 20%, 50%, 60% and 90% acetonitrile by 3, 6, 10, 12, 13 minutes, hold at 90% acetonitrile till 15 minutes, ramp down to 10% acetonitrile by 15.5 minutes and hold till 20 minutes. |
Instrument Name: | Thermo Accela 1250 |
Column Name: | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
Flow Gradient: | hold at 10% acetonitrile for 30 seconds and gradually ramp up to 15%, 20%, 50%, 60% and 90% acetonitrile by 3, 6, 10, 12, 13 minutes, hold at 90% acetonitrile till 15 minutes, ramp down to 10% acetonitrile by 15.5 minutes and hold till 20 minutes. |
Flow Rate: | 200 µl/min |
Solvent A: | 100% water; 0.1 % formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |