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MB Sample ID: SA174236

Local Sample ID:a10
Subject ID:SU001933
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6
Age Or Age Range:8 weeks
Weight Or Weight Range:20-25 g
Gender:Male
Animal Animal Supplier:Model Animal Research Center of Nanjing University
Animal Housing:Standard specific pathogen free (SPF) conditions
Animal Light Cycle:12:12-h light/dark cycle
Animal Feed:food freely
Animal Water:water freely

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Combined analysis:

Analysis ID AN003008 AN003009
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Ultimate 3000 system Thermo Ultimate 3000 system
Column Waters ACQUITY UPLC HSS T3 Waters ACQUITY UPLC HSS T3
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units AUC AUC

Chromatography:

Chromatography ID:CH002230
Chromatography Summary:A 2 μL aliquot of the samples was injected into a Thermo Ultimate 3000 system equipped with an ACQUITY UPLC® HSS T3 column (150 × 2.1 mm, 1.8 μm, Waters, Milford, MA, USA) maintained at 40 °C. The mobile phase for positive mode analysis consists of 0.1% formic acid in water (phase C) and 0.1% formic acid in acetonitrile (phase D), while the mobile phase for negative mode analysis consists of 5 mM ammonium formate in water (phase A) and acetonitrile (phase B). Phase C and D, as well as phase A and B, were mixed and delivered at a flow rate of 0.25 mL/min with a gradient program as follows: 0~1 min; 2% B/D; 1~9 min, 2%~50% B/D; 9~12 min, 50%~98% B/D; 12~13.5 min, 98% B/D; 13.5~14 min, 98%~2% B/D; 14~20 min, 2% D-positive model (14~17 min, 2% B-negative model).
Instrument Name:Thermo Ultimate 3000 system
Column Name:Waters ACQUITY UPLC HSS T3
Column Temperature:40
Flow Gradient:phase A and B were mixed and delivered at a flow rate of 0.25 mL/min with a gradient program as follows: 0-1 min; 2% A/B; 1-9 min, 2%-50% A/B; 9-12 min, 50%-98% A/B; 12-13.5 min, 98% A/B; 13.5-14 min, 98%-2% A/B; 14-20 min, 2% B-positive model (14-17 min, 2% B-negative model)
Flow Rate:0.25ml/min
Solvent A:100% water; 5mM ammonium formate
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase
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