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MB Sample ID: SA262343
| Local Sample ID: | 90410015207 |
| Subject ID: | SU002738 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004308 |
|---|---|
| Chromatography ID | CH003214 |
| MS ID | MS004055 |
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Vanquish horizon LC |
| Column | Thermo Accucore C30 (100 x 2.1mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | NEGATIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH003214 |
| Chromatography Summary: | global lipid separation |
| Methods Filename: | pass1b_lrpneg_lrppos_methods.pdf |
| Instrument Name: | Vanquish horizon LC |
| Column Name: | Thermo Accucore C30 (100 x 2.1mm,2.6um) |
| Column Temperature: | 50C |
| Flow Gradient: | t=0 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=1 min: 40%/60% (%A/%B) @ 0.4 ml/min | t=5 min: 30%/70% (%A/%B) @ 0.4 ml/min | t=8 min: 10%/90% (%A/%B) @ 0.4 ml/min | t=8.2 min: 0%/100% (%A/%B) @ 0.4 ml/min | t=10.5 min: 0%/100% (%A/%B) @ 0.4 ml/min | t=10.7 min: 80%/20% (%A/%B) @ 0.4 ml/min | t=12 min: 80%/20% (%A/%B) @ 0.4 ml/min |
| Flow Rate: | 0.4 ml/min |
| Solvent A: | 40% water/60% acetonitrile; 10 mM ammonium formate; 0.1% formic acid |
| Solvent B: | 10% acetonitrile/90% isopropanol; 10 mM ammonium formate; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
