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MB Sample ID: SA416006
| Local Sample ID: | Titration-10 |
| Subject ID: | SU003933 |
| Subject Type: | Bacteria |
| Subject Species: | Dorea longicatena |
| Taxonomy ID: | 88431 |
| Genotype Strain: | Flavonifractor plautii, Dorea longicatena, Turicibacter sanguinis, Clostridium bolteae, Clostridium symbiosum, Clostridium innocuum, Clostridium clostridioforme, Streptococcus parasanguinis |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN006244 | AN006245 | AN006246 | AN006247 |
|---|---|---|---|---|
| Chromatography ID | CH004735 | CH004736 | CH004737 | CH004738 |
| MS ID | MS005947 | MS005948 | MS005949 | MS005950 |
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | Reversed phase | HILIC | Reversed phase |
| Chromatography system | Shimadzu Nexera X2 | Shimadzu Nexera X2 | Shimadzu Nexera X2 | Shimadzu Nexera X2 |
| Column | Waters Atlantis HILIC (150 x 2 mm, 3 µm) | Waters Acquity BEH C8 (100 x 2.1 mm, 1.7 µm) | Phenomenex Luna NH2 (150 x 2.1 mm, 3 µm) | Waters ACQUITY UPLC BEH C18 (150 x 1.7 mm,2.1 µm) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | IDX | IDX | Orbitrap | IDX |
| MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | POSITIVE | NEGATIVE | NEGATIVE |
| Units | Abudances | Abudances | A | Abudances |
Chromatography:
| Chromatography ID: | CH004735 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Waters Atlantis HILIC (150 x 2 mm, 3 µm) |
| Column Temperature: | 30°C |
| Flow Gradient: | Isocratically with 5% mobile phase A for 1 minute followed by a linear gradient to 40% mobile phase B over 10 minutes |
| Flow Rate: | 250 µL/min |
| Solvent A: | 100% Water; 10 mM Ammonium formate; 0.1% Formic acid |
| Solvent B: | 100% Acetonitrile; 0.1% Formic acid |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH004736 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Waters Acquity BEH C8 (100 x 2.1 mm, 1.7 µm) |
| Column Temperature: | 40°C |
| Flow Gradient: | The column was eluted at a flow rate of 450 µL/min isocratically for 1 minute at 80% mobile phase A, followed by a linear gradient to 80% mobile-phase B over 2 minutes, a linear gradient to 100% mobile phase B over 7 minutes, and then 3 minutes at 100% mobile-phase B. |
| Flow Rate: | 450 µL/min |
| Solvent A: | 95% Water/5% Methanol; 10 mM Ammonium acetate; 0.1% Acetic acid |
| Solvent B: | 100% Methanol; 0.1% Acetic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH004737 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Phenomenex Luna NH2 (150 x 2.1 mm, 3 µm) |
| Column Temperature: | 30°C |
| Flow Gradient: | The column was eluted with initial conditions of 10% mobile phase A and 90% mobile phase B followed by a 10 min linear gradient to 100% mobile phase A. |
| Flow Rate: | 400 µL/min |
| Solvent A: | 100% water; 20 mM ammonium acetate; 20 mM ammonium hydroxide |
| Solvent B: | 75% acetonitrile/25% methanol; 10 mM ammonium hydroxide |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH004738 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Waters ACQUITY UPLC BEH C18 (150 x 1.7 mm,2.1 µm) |
| Column Temperature: | 45°C |
| Flow Gradient: | The column was eluted isocratically at a flow rate of 450 µL/min with 20% mobile phase A for 3 minutes followed by a linear gradient to 100% mobile phase B over 12 minutes. |
| Flow Rate: | 450 µL/min |
| Solvent A: | 100% Water; 0.01% Formic acid |
| Solvent B: | 100% Acetonitrile; 0.01% Acetic acid |
| Chromatography Type: | Reversed phase |
