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MB Sample ID: SA130690

Local Sample ID:M_isoprenaline_4
Subject ID:SU001624
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606

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Collection:

Collection ID:CO001617
Collection Summary:500 μL of culture medium was collected and centrifuged for 5 minutes at 200 x g at 4°C. 20 μL of the supernatant is transferred to a new Eppendorf tube where 180 μL of 50:50, methanol: acetonitrile (ACN) containing 1 μM internal standards (CHAPS, CAPS, PIPES and TRIS) is added. The samples are placed on a shaker for 30 minutes. Samples were then vortexed for 30 seconds before centrifuging at 20,000 x g for 10 minutes at 4°C. 160 μL of supernatant was transferred to a plastic LC-MS vial and stored at -80°C until LC-MS analysis.
Sample Type:Macrophages
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