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MB Sample ID: SA250386
| Local Sample ID: | 1-4 |
| Subject ID: | SU002596 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO002589 |
| Collection Summary: | Murine spleens were harvested and disassociated to single cell suspension at a concentration of 1 x 10^8 cells/mL. Labeling reagent was added at a concentation of 50 μL/mL of sample. Cells were mixed and incubated at RT for 15 mins. Selection cocktail was added to sample at a 70 μL/mL of sample. Cells were mixed and incubated at RT for 15 mins. RapidSpheres were added to sample at a 50 μL/mL of sample. Cells were mixed and incubated at RT for 10 mins. Cells were washed with FACS buffer for 4 times, and were resuspended for downstream metabolomics analysis. |
| Sample Type: | Stem cells |
