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MB Sample ID: SA300850
| Local Sample ID: | 62122_c |
| Subject ID: | SU002912 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 63 - 73 |
| Gender: | Male and female |
| Species Group: | Mammals |
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Collection:
| Collection ID: | CO002905 |
| Collection Summary: | Metabolomics of conditioned media was performed at the Beth Israel Deaconess Medical Center Mass Spectrometry Core according to published protocols (M. Yuan, S. B. Breitkopf, X. Yang, J. M. Asara, A positive/negative ion-switching, targeted mass spectrometry-based metabolomics platform for bodily fluids, cells, and fresh and fixed tissue. Nat Protoc 7, 872-881 (2012)). hVCF (2 x 106) were seeded and grown to confluence and treated with IL-1β (10 ng/ml) for 24h. Conditioned media (1ml) from the vehicle or IL-1β treated hVCF cells were used for the metabolomics analysis. |
| Sample Type: | Cultured fibroblasts |
