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MB Sample ID: SA366553
| Local Sample ID: | 10 C75 100 uM cells |
| Subject ID: | SU003488 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 60 |
| Gender: | Female |
| Cell Strain Details: | BT-474, breast cancer cell line |
| Species Group: | Mammals |
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Collection:
| Collection ID: | CO003481 |
| Collection Summary: | For medium metabolites, 100 µl of medium was mixed with 400 µl of 100% methanol (80% final). For intracellular metabolite samples, the medium was aspirated, and cells were washed with PBS volume matching the volume of the medium. Metabolites were extracted with ice-cold 80% methanol. The volume of the solvent was 500 µl per 6-cm Petri dish (scaled according to the ratio of surface areas for other cell containers). After adding the methanol solution, cells were scraped from the plates, and all the content was transferred to Eppendorf tubes. |
| Collection Protocol Filename: | DM_metabolomics_samples.txt |
| Sample Type: | Breast cancer cells |
| Collection Method: | 80% methanol extraction |
| Storage Conditions: | -80℃ |
| Collection Vials: | 1.5 ml plastic centrifuge tubes |
| Storage Vials: | 1.5 ml plastic centrifuge tubes |
