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MB Sample ID: SA385752
Local Sample ID: | HSA-000019576 |
Subject ID: | SU003630 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | 15 mo |
Gender: | Male |
Species Group: | Mammals |
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Collection:
Collection ID: | CO003623 |
Collection Summary: | Microglia isolation was performed using the Magnetic-activated cell sorting (MACS) system (Miltenyi Biotec). No CD11b enrichment step was performed as the intrinsic mKate2 reporter is only expressed in TREM2 expressing (microglia) cells. Hence, fluorescence-activated cell sorting was performed based on the mKate2 fluorescence signal and sorted into low, mid and high expressing subpopulations, as a proxy for the TREM2 expression of individual cells. |
Sample Type: | microglia |
Storage Conditions: | -80℃ |