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MB Sample ID: SA136085

Local Sample ID:P 204
Subject ID:SU001684
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:22 strains from the Collaborative Cross (CC) mouse panel
Gender:Female

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Combined analysis:

Analysis ID AN002640
Analysis type MS
Chromatography type Normal phase
Chromatography system Waters Acquity UPLC
Column Luna Silica (150 x 2mm,3┬Ám)
MS Type ESI
MS instrument type QTRAP
MS instrument name ABI Sciex API 4000 QTrap
Ion Mode POSITIVE
Units micromolar

MS:

MS ID:MS002452
Analysis ID:AN002640
Instrument Name:ABI Sciex API 4000 QTrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:Analytes were monitored using electrospray ionization in positive-ion mode with multiple reaction monitoring (MRM) of precursor and characteristic production transitions as shown in MS_protocol.pdf. The parameters for the ion monitoring were as follows: spray voltage, 4.5 kV; curtain gas, 15; GS1, 60; GS2, 50; CAD gas, medium; Nitrogen (99.95% purity) was used as the source and collision gas. Integration and quantification of values was done using Analyst 1.6.2 software (AB SCIEX, Singapore). Standard linearity was calculated using linear regression model. Please see LC_protocol.pdf and MS_protocol.pdf for additional details.
Ion Mode:POSITIVE
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