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MB Sample ID: SA298973
| Local Sample ID: | 70_T4 |
| Subject ID: | SU002893 |
| Subject Type: | Mammal |
| Subject Species: | Sus scrofa |
| Taxonomy ID: | 9823 |
| Age Or Age Range: | ~4 months |
| Weight Or Weight Range: | 35-45 kg |
| Gender: | Male |
| Animal Animal Supplier: | TOPIGS TN70, Tojapigs, the Netherlands |
| Animal Light Cycle: | 12 hours |
| Animal Water: | ad libitum |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004533 |
|---|---|
| Chromatography ID | CH003406 |
| MS ID | MS004280 |
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters ACQUITY UPLC BEH C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Focus |
| Ion Mode | NEGATIVE |
| Units | Peak area |
MS:
| MS ID: | MS004280 |
| Analysis ID: | AN004533 |
| Instrument Name: | Thermo Q Exactive Focus |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Raw MS data were converted into mzML using the MSConvert tool of Proteowizard (version 3.0.20247). Peak picking was performed with El_Maven (El_Maven, Elucidata, Massachusetts, USA, version 0.12.0). Metabolites were identified using an in-house library containing exact mass and retention time. The mass accuracy during data processing in El Maven was set at 10 ppm. Calculation of abundances was done in the LC-MS Workflow of El_Maven. Raw abundances (peak area values) for each metabolite were corrected for internal standard (Myristic acid d27). These were corrected for urine volume. |
| Ion Mode: | NEGATIVE |
