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MB Sample ID: SA006152

Local Sample ID:Stationary phase-S2
Subject ID:SU000135
Subject Type:Bacterial cells
Subject Species:Acidipropionibacterium acidipropionici
Taxonomy ID:1748
Genotype Strain:GCMCC1.2230/WSH1105
Species Group:Microorganism

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Sample Preparation:

Sampleprep ID:SP000132
Sampleprep Summary:After quenching at –40°C, the cells were pelleted in a centrifuge (4,000 × g, ?4°C, 10 min). The pellets were washed with methanol solution and resuspended in 1 mL 35% perchloric acid to extract the metabolites from the cells. The mixture was frozen in liquid nitrogen and thawed three times. The supernatant was neutralized by adding K2CO3 solution with an initial concentration of 5 M, and an extract containing all metabolites was collected via centrifugation at 10,000 × g for 5 min at –4°C
Processing Method:Homogenization and solvent removal
Processing Storage Conditions:on ice
Extraction Method:35% perchloric acid and 5 M K2CO3
Extract Storage:-80°C
Cell Type:wildtype cells, genome shffled cells