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MB Sample ID: SA006164
| Local Sample ID: | 140826bmssa09_1 |
| Subject ID: | SU000136 |
| Subject Type: | Bacterial cells |
| Subject Species: | Salmonella enterica;Escherichia coli |
| Taxonomy ID: | 28901;562 |
| Genotype Strain: | DM3480|DM14100|Salmonella enterica subsp.enterica serovar Typhimurium str.LT2|K12MG1655| ridA3::MudJ|ridA3::tn10ΔyoaB624::catSTM1549-26::kan| | |yjeF::kan |
| Species Group: | Microorganism |
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Sample Preparation:
| Sampleprep ID: | SP000133 |
| Sampleprep Summary: | - |
| Extraction Method: | 1 mL of cold, degassed extraction solvent (3:3:2 acetonitrile:isopropanol:water v/v/v) was added to each sample, vortexed for 10 seconds, then shaken at 4C for 4 min. Samples were spun down using a centrifuge at 14 rcf for 2 min. Aliquots (450 uL) were transferred to 1.5 mL eppendorf tubes and dried down using a Labconco centrivap evaporator. |
| Extract Storage: | -80C |
| Sample Derivatization: | 10 uL of 40 mg/mL methoxyamine hydrochloride in pyridine was added to room temperature samples and shaken for 90 min at 30C. 91 uL of silyating agent (MSTFA) containing a set of 13 C8 - C30 fatty acid methyl ester internal standards was added and samples were shaken for 30 min at 37C. Derivatized samples were transferred to glass vials with inserts for analysis. |
| Sample Spiking: | Set of 13 C8 - C30 fatty acid methyl esters (FAMES) |
