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MB Sample ID: SA011797

Local Sample ID:A_M3e
Subject ID:SU000278
Subject Type:Bacterial cells
Subject Species:Sinorhizobium meliloti
Taxonomy ID:382
Genotype Strain:Rm2011 (SU47 str-3)|SmA818|RmP3009|RmP2917
Human Ethnicity:wild type|Rm2011 lack pSymA|Rm2011 lack pSymB|Rm2011 lack pSymA and pSymB | a tRNAarg and the smb20996-engA operon from pSymB were integrated to chromosome in strains RmP3009 and RmP2917
Species Group:Bacteria

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Sample Preparation:

Sampleprep ID:SP000285
Sampleprep Summary:-
Sampleprep Protocol Filename:Fei,_F.,_Bowdish,_D._M._E.,_&_McCarry,_B._E._(2014)._Analytical_and_Bioanalytical_Chemistry,_406,_3723–3733.
Extraction Method:cell pellet extracted with 2:2:1 MeOH/EtOH/H2O with beadbeating
Extract Storage:-80ºC freezer
Sample Resuspension:cell extracts suspended 60%v/v acetonitrile/water
Sample Spiking:L-methionine-d3, L-tryptophan-d5 as standards for recovery determination ; L-phenylalanine-d8, diphenylalanine and glycine-phenylalanine as internal standards for peak area normalization
Cell Type:Gram negative bacterium
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