Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA028725

Local Sample ID:	Plasma_UT_Rep5
Subject ID:	SU000575
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Mammals

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Sample Preparation:

Sampleprep ID:	SP000582
Sampleprep Summary:	Sample preparation Plasma: Plasma samples were stored at -80°C until the day of the analysis. Metabolite extraction was performed by adding 300μL of cold LC-MS grade methanol (containing ISTDs) to 100μL of plasma. Samples were centrifuged to pellet the precipitate (13,000g, 15 min, 4°C). An aliquot of 60μL of the supernatants was transferred to 2 separate LC vials for Reversed-phase chromatography and positive and negative ESI mode detection. Extracts were evaporated to dryness using a speed vac system. The extracts were reconstituted using 50μL of methanol on the day of analysis. In case of the HILIC analysis (positive and negative ESI detection), the extracts were reconstituted using 50μL acetonitrile: water in 8:2 ratio. Urine: Urine samples were stored at -80°C until the day of the analysis. Metabolite extraction was performed by adding 180μL of Acetonitrile (containing ISTDs) to 20μL of urine. Samples were centrifuged to pellet the precipitate (13,000g, 15 min, 4°C). An aliquot of 40μL of the supernatants was transferred to 4 separate LC vials (RP and HILIC mode, positive and negative ESI analysis).

Sampleprep ID:

SP000582

Sampleprep Summary:

Sample preparation Plasma: Plasma samples were stored at -80°C until the day of the analysis. Metabolite extraction was performed by adding 300μL of cold LC-MS grade methanol (containing ISTDs) to 100μL of plasma. Samples were centrifuged to pellet the precipitate (13,000g, 15 min, 4°C). An aliquot of 60μL of the supernatants was transferred to 2 separate LC vials for Reversed-phase chromatography and positive and negative ESI mode detection. Extracts were evaporated to dryness using a speed vac system. The extracts were reconstituted using 50μL of methanol on the day of analysis. In case of the HILIC analysis (positive and negative ESI detection), the extracts were reconstituted using 50μL acetonitrile: water in 8:2 ratio. Urine: Urine samples were stored at -80°C until the day of the analysis. Metabolite extraction was performed by adding 180μL of Acetonitrile (containing ISTDs) to 20μL of urine. Samples were centrifuged to pellet the precipitate (13,000g, 15 min, 4°C). An aliquot of 40μL of the supernatants was transferred to 4 separate LC vials (RP and HILIC mode, positive and negative ESI analysis).