Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA075620

Local Sample ID:	M_1576_120
Subject ID:	SU001154
Subject Type:	Mammal
Subject Species:	Ovis aries
Taxonomy ID:	9940
Age Or Age Range:	Gestational days 125-birth
Species Group:	Mammals

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Sample Preparation
Analysis
NMR

Sample Preparation:

Sampleprep ID:	SP001161
Sampleprep Summary:	Saline buffer was created by weighing 9 g of NaCl into a 1 L volumetric flask. Next, 100 mL of D2O was added and filled up to 1 L with water and shaken until dissolved. Samples were thawed on ice and centrifuged at 3,000 g for 20 min at 4 °C. Two hundred mL of serum was mixed with 400 mL of Saline buffer and centrifuged for 10 min at 3000 g at 4 °C. For each sample, 500 mL of the resulting supernatant was transferred into a 5 mm SampleJet NMR tube (Bruker Biospin, Billerica, MA, USA).

Sampleprep ID:

SP001161

Sampleprep Summary:

Saline buffer was created by weighing 9 g of NaCl into a 1 L volumetric flask. Next, 100 mL of D2O was added and filled up to 1 L with water and shaken until dissolved. Samples were thawed on ice and centrifuged at 3,000 g for 20 min at 4 °C. Two hundred mL of serum was mixed with 400 mL of Saline buffer and centrifuged for 10 min at 3000 g at 4 °C. For each sample, 500 mL of the resulting supernatant was transferred into a 5 mm SampleJet NMR tube (Bruker Biospin, Billerica, MA, USA).