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MB Sample ID: SA078220
| Local Sample ID: | P10 |
| Subject ID: | SU001195 |
| Subject Type: | Plant |
| Subject Species: | Brassica nigra |
| Taxonomy ID: | 3710 |
| Age Or Age Range: | 4 weeks old |
| Height Or Height Range: | 15-20 cm |
| Species Group: | Plants |
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Sample Preparation:
| Sampleprep ID: | SP001203 |
| Sampleprep Summary: | Each sample (10-12 mg) was extracted in 1 ml of cold chloroform:methanol:H2O (20:60:20 v/v), including 7.5 ng/µl of the isotope-labelled internal standard, SA-D4. Extracts were agitated with a 3 mm tungsten carbide bead for 3 min and centrifuged at 20,800 × g for 10 min at 4°C. The supernatant (200 μl) was dried and processed for GC/MS analyses |
| Processing Storage Conditions: | On ice |
| Extract Storage: | -80℃ |
| Sample Derivatization: | Dry extracts were derivatized with methoxyamine and MSTFA |
