Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA090482

Local Sample ID:	SPIN_5neg_T21_7-L16-038
Subject ID:	SU001310
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Age Or Age Range:	0.5 - 76.5
Gender:	Male and female
Species Group:	Mammals

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Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP001318
Sampleprep Summary:	For CSF analyses, a volume of 20μL of was extracted in 180μL of ice-cold methanol:acetonitrile:water (5:3:2). Subsequently, these solutions were vortexed for 30 minutes at 4°C. Insoluble proteins were pelleted by centrifugation (10 minutes at 4°C and 12,000 g) and supernatants were collected and stored at -80°C until analysis. For quantitative analysis of kynurenine pathway (KP) metabolites, supernatants were spun in a Speedvac until dry and resuspended in 0.1% formic acid in water as previously described (PMID: 30213797, 30143553).

Sampleprep ID:

SP001318

Sampleprep Summary:

For CSF analyses, a volume of 20μL of was extracted in 180μL of ice-cold methanol:acetonitrile:water (5:3:2). Subsequently, these solutions were vortexed for 30 minutes at 4°C. Insoluble proteins were pelleted by centrifugation (10 minutes at 4°C and 12,000 g) and supernatants were collected and stored at -80°C until analysis. For quantitative analysis of kynurenine pathway (KP) metabolites, supernatants were spun in a Speedvac until dry and resuspended in 0.1% formic acid in water as previously described (PMID: 30213797, 30143553).