Return to study ST001380 main page

MB Sample ID: SA100902

Local Sample ID:Sample_2
Subject ID:SU001454
Subject Type:Other
Subject Species:Homo sapiens
Taxonomy ID:9606

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:SP001462
Sampleprep Summary:To extract metabolites and lipids from serum samples, we mixed 20 µL of serum with an extraction solution for metabolomics analysis and 10 µL for lipidomics in 96-deep well plates. For lipidomics analysis, we utilized 100 µL of 2-propanol/methanol (6:1, v/v); and for metabolomics analysis, 100 µL of methanol/acetonitrile/water (5:3:1, v/v/v). After 10 min of vortexing, 800 rpm, precipitated proteins were separated by centrifugation for 20 min at 4 °C and 4000 rcf; supernatants were stored at -80 °C prior the analysis.